MBP fusion is an effective and generalizable approach to enable crystallographic studies of engineered binding proteins that are otherwise difficult to crystallize #Monobody #Aggregation #MaltoseBindingProtein doi.org/10.1107/S205...

Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies This article describes the transition of the Glass laboratory from X-ray crystallography to single-particle cryo-electron microscopy (cryo-EM) for structural studies of ATAD2B, a large AAA+ ATPase- and bromodomain-containing protein involved in chromatin regulation. It outlines practical strategies for protein expression, sample preparation, data processing and model building, offering a comprehensive workflow and troubleshooting guide for new cryo-EM users working with large, flexible macromolecular complexes.

Hassan Zafar et al.: Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies #CryoElectronMicroscopyCryoEM #StructuralBiology #SingleParticleAnalysis @uvmvermont...#IUCr https://journals.iucr.org/paper?S205979832600080X

Cheat sheet for ChimeraX.

ChimeraX cheat sheet brought to you by Alejandro Pereira. www.rbvi.ucsf.edu/chimerax/dat...

Happy 100th, structural biology pubs.aip.org/aca/sdy/arti...

Delightful perspective by George Phillips and Ed Lattman. Have a piece of cake everyone.

Just out: Covalent chemical probes

How can you find consistent biological 3D structures in heavily contaminated electron microscopy data sets without using any prior knowledge? #CryoEM #SingleParticle #Reconstruction doi.org/10.1107/S205...

Potent β-lactam-based tyrosyl-DNA phosphodiesterase 1 inhibitors identified by a virtual screen - Scientific Reports Scientific Reports - Potent β-lactam-based tyrosyl-DNA phosphodiesterase 1 inhibitors identified by a virtual screen

Our latest work on the discovery of new inhibitors of tyrosyl-DNA phosphodiesteras 1 (TDP1) is now published in Scientific Reports.

www.nature.com/articles/s41...

Scientist presenting talk

Scientist presenting talk

Scientist presenting talk

Scientist presenting talk

Great Hot Structures session at #ACA2025!
ACA Etter Student Award winner Sarah Narehood, @biophyskrys.bsky.social, Nikolas Capra, Rachel Kretsch, Debanu Das, Daniel Richman
@acastructuralsci.bsky.social

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE We introduce a new approach to probabilistic multi-volume single-particle cryo-EM ab initio 3D reconstruction for simultaneous estimation of the relative particle 3D orientations and partitioning of the particles into groups with distinct structural states.

Cong T. S. Van et al.: Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE #CryoEM #SingleParticle #Reconstruction ... #IUCr https://journals.iucr.org/paper?S2059798325005686

Activity and structure of human (d)CTP deaminase CDADC1 | PNAS Vertebrates have evolved an understudied protein termed CDADC1 (NYD-SP15) that contains an inactive N-terminal and active C-terminal DCTD-like doma...

Activity and structure of human (d)CTP deaminase CDADC1 | PNAS www.pnas.org/doi/10.1073/...

Are you new to #CryoEM, or looking for a review of the basics?

You might enjoy our TRPV1 case study! It covers preprocessing, particle curation, refinement, and more! You can also test your skills with the advanced exercises at the end of the study.
guide.cryosparc.com/processing-d...

#CryoSPARC

Welcome to the participants of the 2025 #CryoEM course at Cold Spring Harbor Lab @cshlaboratory.bsky.social ! It's going to be a great 2 weeks immersed in all aspects of single particle cryoEM methods.

Impact of structural biology and the protein data bank on us fda new drug approvals of low molecular weight antineoplastic agents 2019–2023 Oncogene - Impact of structural biology and the protein data bank on us fda new drug approvals of low molecular weight antineoplastic agents 2019–2023

Impact of the PDB on drug approvals
Open access to the PDB facilitated discovery/development of 100% of the 34 new low molecular weight, protein-targeted, antineoplastic agents approved by the US FDA 2019–2023.
Read more in Oncogene
www.nature.com/artic...