Recent progress in quantitative phosphoproteomics www.tandfonline.com/...

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#proteomics #prot-paper

Yes, our lab also encountered the same issue not too long ago. Sometimes the beads aren’t resuspended thoroughly and more storage buffer is taken instead of the actual beads. Other times, we were accidentally using 2-3X more beads and getting low recovery. Lesson learned to always weigh beads!

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Summary of my quant proteomics #Asilomar2023 talk:
1. sample space >>> throughput(e.g. chemical compounds in drug screening, cell types in biology, etc)
2. Interesting things are typically rare
3. Followup work will filter out false positives, but you can never recover a false negative