🔬At #MFS25, Giulia Zanetti from @TheCrick unveiled the molecular architecture of the early secretory pathway using cryo-ET & super-resolution microscopy resolving the debate on COPII vesicles and mapping ER-to-Golgi transport in human cells.
🔗https://mfs2025.com/
We're looking for an awesome computational #postdoc to join our group @PioneerCampus. You'll explore native cells with #cryoET, get long-term funding (5+ years), and live in Munich! For more info, see our new Cell Architecture Lab website:...
I attended this course last year and cannot recommend it enough!
Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
This revolutionised our segmentation of coated membranes.
Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!
Yes! we reproducibly get ~150nm thin lamella with very few failures and regularly run sessions for ~48hrs.
We have the Aquilos2 which is fab!
Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT
🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo
Ready for BBCs #AQuestionofScience here @crick.ac.uk 🔬🧬🥼🧪🦠
We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin.
It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...
I am thrilled to announce my and @giuliazanetti.bsky.social's latest review "Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway" in @natrevmcb.bsky.social with @springernature.com.
Check it out here!
rdcu.be/eeY1J
#COPII #CryoEM #TeamTomo #MembraneTrafficking #ERES
Does anyone have any idea what this wavey guy is? 🌊🌊🌊🌊#TeamTomo #CryoET
In-situ tomogram of milled RPE-1 cells.
@giuliazanetti.bsky.social something worth trying with our data maybe?
Exciting news! #MembraneTrafficking online seminars with @felixmendu.bsky.social @franbottanelli.bsky.social and me are back!
Check the spectacular Jan-April 2025 lineup.
Details (Zoom links, calendar invites) on the mailing list, Slack group & website: felixcampelo.wixsite.com/membranetraf...
Both very well deserved!
Lets play...
What
Am
I
Looking
At??
Low mag tomogram of milled RPE-1s.
Any guesses of whats going on here? #TeamTomo
Prize? idk... bragging rights? The feeling of superiority?
I'll collect a high mag tomo tomorrow, so the answer may actually be revealed! (mysteries mysteries mysteries).
We won’t be there I’m afraid, but we are always happy to chat!
This scheme shows the cargo adaptor FHF in the middle with arrows pointing to distinct microtubule and actin-based motors (dynein-dynactin, KIFC1, KIF1C and myosins V, IX and X as well as Tropomyosin 1). It also shows a link to Rab5 marked early endosomes. The hypothesis is that FHF coordinates multi motor binding at the early stages of endocytosis. The PhD project would address the hierarchy of motor binding and how these motors coordinate to allow the newly formed vesicle to transition from the actin to MT cytoskeletal network.
If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪
This year’s UK Membrane Trafficking Meeting is on Dec 16th at King’s College London (Guy’s Campus, London Bridge).
Registration closes 9th December. Sign up is here.
estore.kcl.ac.uk/conferences-...
Thanks go to @J_Cell_Sci for sponsoring student/postdoc prizes!
📢 Announcing the Membrane Trafficker Starter Pack! This list highlights researchers advancing our understanding of membrane trafficking pathways. This is just a beginning—help expand the list!
go.bsky.app/J9GvL3S
Could I please be added? 😇
👌 that cell placement 😍
Honestly no idea how this happened. The training data looked fine! 🤯
Could I please be added?
