Refining #AKI #Diagnosis: Is There a Return on the Investment?
https://doi.org/10.1016/j.ekir.2026.106463
Original Article: https://doi.org/10.1016/j.ekir.2025.09.029 & https://doi.org/10.1016/j.ekir.2026.103772
#KIRCommentary
Case-Control and Longitudinal Study of #Sex #Steroids in Male Patients with #CKD Before and After Kidney #Transplantation
#VisualAbstract by @KajareeG
www.kireports.org/article/S2468-0249(26)02...
Prospective Cohort Study of #Felzartamab in #Rituximab-Resistant Primary #MembranousNephropathy
#VisualAbstract by @RafaelLpezMrqu2
www.kireports.org/article/S2468-0249(26)02...
@IstitutoNegri @TrilliniMatias
#CognitiveImpairment Predicts #Adverse Outcomes in Chronic Kidney Disease #CKD
#VisualAbstract by Brian Rifkin
www.kireports.org/article/S2468-0249(26)02...
Please check the skytorial on genetic testing in ADPKD
A big thanks to all my mentors
bsky.app/profile/did:...
20/20
Huge thanks to all my mentors
Happy learning!
The link for the blog
www.kireportscommunity.org/post/genetic...
19/20@ISNkidneycare The answer is D
The long-range locus-rich PCR technique is excellent in accuracy but laborious. The following table illustrates the differences between various techniques.#Nephsky #Medsky
For more information read following article
doi.org/10.1080/1473...
18/20 @ISNkidneycare Let’s go for a quiz. #Nephsky #Medsky
Which method of NGS is outstanding in accuracy?
A) Whole exome sequencing
B) Whole genome sequencing
C) DNA capture-based targeted screening
D) LR-based PCR-targeted screening
17/20 What are pseudogenes? They are nonfunctional, resemble functional genes, cannot produce proteins, and lack regulatory elements. High sequence similarity to functional genes and located near areas of segmental duplication makes more difficult to analyze.
#Nephsky #Medsky
16/20 Why is PKD1 genetic analysis complex? #Nephsky #Medsky
PKD-1 analysis = Technical nightmare
Contains two parts (1 - 33 and 33 - 46 exons)
GC-rich
6 pseudogenes are present.
False positives + mapping errors
15/20 Let us learn more about prenatal genetic testing, involves performing intracytoplasmic sperm injection (ICSI) on the ovum on day three at the blastocyst stage, during which 5-10 trophectoderm cells are taken and sent for genetic testing to identify ADPKD-negative embryos. #Nephsky #Medsky
14/20 Genetic testing is beneficial not only in diagnosis but also to exclude the diagnosis in ADPKD, especially.
To select the kidney donor within the family
Before preimplantation genetic testing
#Nephsky #Medsky
13/20 The various indications for genetic testing are clinically needed, and some are evolving. For more information, reach out this article #Nephsky #Medsky
doi.org/10.1016/j.ki...
12/20Though a clinico-radiological hint is suffice for diagnosis, genetic testing helps in prognostication through PROPKD score #Nephsky #Medsky
doi.org/10.1681/asn....
11/20Did you know about the importance of allelic heterogeneity (phenotypic heterogeneity) of the disease? #Nephsky #Medsky
🔴 Truncating (PT) → severe, early
🟢 Non-truncating (NT) → milder, late
But exceptions exist: phenotype ≠ genotype always
doi.org/10.1681/ASN....
10/20 We will recap the importance of the common ADPKD genes, polycystin1 and polycystin2. #Nephsky #Medsky
9/20Do you know various important genes for differential diagnosis of ADPKD presenting with the cystic kidney morphology? #Nephsky #Medsky
8/20 Do you know various genes involved in the ADPKD phenotype, with their incidence patterns and clinical severity? #Nephsky #Medsky
genetic architecture
✔️ PKD1 (~85%)
✔️ PKD2 (~15%)
✔️ Minor genes (IFT140, GANAB, DNAJB11…)
For more information read
www.ajkd.org/action/showP...
7/20
Why is genetic testing not first-line in typical ADPKD?
a) Low sensitivity
b) Lack of mutations
c) Imaging + Family history often suffices.
d) High false negatives . #Nephsky #Medsky
6/20 Did you know ADPKD accounts for approximately what proportion of patients with ESKD?
<1%
2-3%
5-10%
>20%
#Nephsky #Medsky
5/20 What are the different types of next-generation sequencing?
For more info please read
doi.org/10.1002/mgg3...
#Nephsky #Medsky
4/20 Quick check!
What is the biggest advantage of NGS?
Simultaneous and high-throughput screening
Easily available and affordable
Single gene analysis
No need for DNA
#Nephsky #Medsky
3/20 What are the developments in genetic testing?
Genetic testing underwent a sea change from slow and laborious Sanger analysis to high-throughput simultaneous screening of multiple disease genes after the introduction of Next Generation Sequencing (NGS). @ISNkidneycare
#Nephsky #Medsky
2/20 Our author is Dr Saivani@ (@DrSaiVani1, @drsaivani.bsky.social)
Consultant Nephrologist, Kurnool Kidney Care, Kurnool, India. Today’s topic is
Genetic testing (Next Generation Sequencing)—A Sherlock Holmes in discovering the truth of #Nephsky, #Medsky
1/20 Hello everyone, welcome to www.kireportscommunity.org #Nephsky #Medsky Welcome to a #skytorial brought to you by @KIReports
#Cardiac Gated Diffusion-weighted #MR Imaging Assessment of Kidney #Function in #KidneyCancer Patients
#VisualAbstract by Denisse Arellano
www.kireports.org/article/S2468-0249(26)02...
19/
💬 Curious what you think:
Would you actually use a bedside GFR device
if it was available in your unit? 🖥️
Or are we too comfortable with creatinine chaos? 🩸
#NephSky
18/
🧠 Take-home:
tGFR is a promising step toward making measured GFR clinically usable.
But it may not be ready for routine use yet, given the lack of validation against gold-standard mGFR and technical issues that could affect real-world reliability.
#NephSky
#MedSky
17/
tGFR still needs:
IV access 💉
A device 📱
Time for signal acquisition - it’s not replacing creatinine in your OPD tomorrow. 🕤
🔄 Can be trialed in: Oncology dosing, ICU / dynamic kidney function, Transplant evaluation, low muscle mass.
Basically where eGFR is most unreliable.
#NephSky
16/ Is a 30% margin (P30) acceptable when we’re dosing chemo or nephrotoxins?
⁉️How would this perform in real patients - AKI, ICU, Edema, paeds?
⁉️Are we ready to move from a $10 creatinine test → device + tracer-based system?
Accuracy is one thing. Adoption is a different battle.
#MedSky