I should add this is being processed in Cryosparc ATM. I’ll try 3D classification after and hope that can clear up some of the misaligned particles but would be nice to be able to select on metadata.

#cryoEM folks, what’s your favorite trick for focused refinements? I have ~4 million symmetry expanded particles and it’s clear some will wander away too far from their correct alignment. Is there a way to select particles based on magnitude of alignment changes?

Hi everyone! I’m a postdoc at the University of Minnesota working on viral replication and host-pathogen interactions.

I would like to be added please!

Awesome work! Have you or others attempted to put any of the disulfides into a NabFab?

#cryoEM folks, why are facilities forgoing globus in favor of physical hard disks? - sincerely, impatient postdoc

Native CTD reading when?