Purée, il a vraiment dit ça 1er degré...

1/ Excited to share our preprint! 🥳

Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine

www.biorxiv.org/content/10.6...

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬

Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

SOLEIL synchrotron

Rooftop view of Paris

The samples are safe in the baby seat

Panthéon, 5th arrondissement

Coming back from the "traditional" yearly trip to Paris 🥖
Great scientific interactions at IBPC @bpm7099.bsky.social and SOLEIL synchrotron, plus the warm and sunny ☀️ weather, perfect combo!
Our little sample Dewar even got it's own baby seat 💺
See you next time!

Hello everyone! If you want to make sure your paper is posted here, the bot scrapes for the following keywords in the title and abstract from bioRxiv and PubMed:
-cryoem
-cryo-em
-cryogenic electron microscopy
-cryoet
-cryo-et
-cryogenic electron tomography
-structural biology

Check out this cool integrative structural biology story from my colleague @computingcaitie.bsky.social on mammalian cilia 🍟
Beautiful tomos of the ciliary base !

Spent too much time in situ and forgot the level of detail you get with SPA 🍩
#cryoEM

Picture of an Arabidopsis flower

When work follows you all everywhere ... Arabidopsis at my family's place ☘️
Happy Easter 🐣🐰

Congratulations @ecleroy.bsky.social 🥳! Very cool story :)

🚨 Preprint alert!

We solved the structure of human NSUN2 bound to Asp tRNA, revealing how it installs m⁵C and recognizes RNAs
#CryoEM #RNABiology

A collab between Casañal & Gullerova labs, with @miclass.bsky.social and me working side by side as co-first authors

🔗 doi.org/10.64898/202...
(0/7🧵)

You want to work on carbon concentration mechanisms in algae 🫑? Go work with my (soon-to-be-former) colleague
@manondemulder.bsky.social in Vienna @gmivienna.bsky.social !
Cryo-ET 🔬 and funky bugs 🦠

#CryoET #CryoEM

Excited to announce I’m starting a new lab at @gmivienna.bsky.social (Vienna BioCentre) in Sept 2026

We study how carbon fixation is inherited and maintained in algae using cryo-ET, imaging, environmental sampling&more

Looking for people who want to build the lab together, get in touch!

#teamtomo

Microscopy preprints - applications in biology - FocalPlane Microscopy preprints - applications in biology - News

Our #preprint list is now up on FocalPlane.
Start your week feeling inspired by the latest research using microscopy to answer questions in biology. Let us know if you have any recommendations for us to add.

focalplane.biologists.com/2026/03/09/m...

Happy to share our new preprint on the mechanism of human tRNA 3' CCA maturation! This project was spearheaded by Bernhard Kuhle in my group, with contributions from many others and a great collaboration with the group of Peter Rehling (UMG). See highlights below!

www.biorxiv.org/content/10.6...

There is a week left to apply for two available positions in my lab @ipbhalle.bsky.social

- PhD: ipb-halle.mhm.jobs/12-phd-posit...

- Postdoc: ipb-halle.mhm.jobs/13-postdocto...
Earliest start date: 01/05/2026.

We are interested in plant immune receptor biochemistry and evolution. Please share!

Had a great time in Zurich for the annual LS² meeting. Nice gathering of the swiss scientific community, always cool to see what the neighbors are doing 😜

Thank you Ahmad! That's a good question, we are not entirely sure! The position could be compatible for potential trans-acting factors (present in chlamy) binding there for specific translation initiation events (5'UTR binding maybe), a bit similar to what was recently described for yeast mitoribos

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome www.biorxiv.org/content/10.64898/2026.02...

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome www.biorxiv.org/content/10.64898/2026.02.07.704542v1 #cryoEM

For this work, we used the publicly available Chlamy dataset (www.ebi.ac.uk/empiar/EMPIA...) which was recently published.
www.cell.com/molecular-ce...
Check it out!
Thanks to the co-authors @phaips.vd.st, Philippe Lehner, @pfeffercryolab.bsky.social , Lukas Kater and @cellarchlab.com !

It was a fun dive into the chloroplast! After working so much on mitoribosomes (which are weird and divergent!) I always assumed chlororibosomes were more conserved and bacterial-like. Turns out they might be more diverse than expected!
It was fun, but now, back to mitochondria!

We observed that these protein extensions localize around the mRNA exit and entry channels, extending them to create more stable mRNA threading. This likely enhances polysome stability and could play a role in chloroplast-specific translation regulation.

Combined with single-particle cryo-EM, we identified all the proteins in this “hat.” Turns out it’s more of an arm 🦾, rooting from the body it reaches for the head! It is composed of large extensions in conserved proteins S2, S3, S4, and S5, as well as three specific proteins.

Interestingly, these chlororibosomes are loosely bound to the thylakoid membranes. High heterogeneity made classification challenging to find clean thylakoid-bound ribosomes! The comparison with cytosolic and mitoribosomes shows how rigidly the latter bind membranes compared to the chlororibosomes.

Historical perspective 👴: this project predates me! In 2021, I joined @cellarchlab.com (at MPI) to work on mitoribosomes, but Ben tried to lure me with a mysterious "hatted" chlororibosome. @pfeffercryolab.bsky.social did an initial average 8 y.a. as a postdoc, but it stayed in a drawer until now!

Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵

For #ECR’s:

DBG’s Hot Tpoic Workshop
on Cryo-Electron Tomography + FIB-milling

13 – 15 April 2026
In @cellarchlab.com 's lab @biozentrum.unibas.ch

Organised by @tamb-o.bsky.social, Karen Zinzius, @fannyleblanc.bsky.social

Details: www.deutsche-botanische-gesellschaft.de/en/about-us/...

Am I the only one increasingly annoyed by cryo-EM papers not showing *any* experimental density in figures? Sometimes not even in the supplementary material??

Check this new study on carbon concentration mechanisms!

See how the pyrenoid changes by modifying Rubisco linkers in our favorite alga, Chlamy 💚!

My desk mate @phaips.vd.st spent a lot of time collecting & carefully analyzing these beautiful tomograms 🔬.

Congrats to all the authors!

You like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint!

What a great collaboration this has been!