Please join the chorus of voices, including my own, in support of the talented, hardworking and dedicated scientists and staff at NIH. They serve their country by deploying their training and skills to improve the health and quality of life of all Americans 🙏 🇺🇸

NIH staff break ranks in open letter to Director Jay Bhattacharya Bethesda Declaration urges reversal of recent policies, including funding cuts

Article by @cenmag.bsky.social on the #BethesdaDeclaration by NIH scientists and staff released earlier this morning:

cen.acs.org/policy/resea...

Wow #glycotime from @msollogoub.bsky.social @vivesgui.bsky.social and colleagues 👏🏻👏🏻👏🏻🎉🤩 Super congrats! ⤵️ #chemsky

Too cool to be true? 😎 Nope! GlycoShape ReGlyco runs on PyMol through the GlycoMe interface 🥰😍 check out this fab #glycotime tool from Hiren @glyco.me and Ojas @ojas-singh.bsky.social ⤵️🥳🥳🥳🎉🎉

Well, it turns out that you can actually make PyMOL do quite a lot of stuff. Here's the full workflow, integrating the amazing ReGlyco into PyMOL to re-glycosylate the LDLA linker. There's some serious magic needed to get it running as fast as it is within PyMOL. @elisafadda.bsky.social

Improved ESI-MS Sensitivity via an Imidazolium Tag (DAPMI-ITag) for Precise Sialic Acid Detection in Human Serum and CMAH-Null Mouse Tissues Sialic acids (Sias), consisting primarily of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), play crucial roles in many biological processes. The detection and quantification of Sias are essential for understanding their roles in health and disease progression. Although numerous techniques have been developed to enhance the specificity and sensitivity of Sias analysis, traditional methods such as derivatization with fluorescent tags coupled with HPLC-MS analysis often suffer from low limits of detection, limiting the quantification of Sias in trace samples. Here, we introduce DAPMI, a novel imidazolium-based ITag for sensitive Sia detection. We demonstrate its utility in the detection and quantification of Sia composition in human serum, and in different tissues from CMAH (cytidine monophosphate-N-acetylneuraminic acid hydroxylase) knockout mice, using ESI-MS analysis and with a limit of detection (LOD) down to the low fmol range. The results showed that both Neu5Ac and Neu5Gc were present in varying proportions in wild-type mice and CMAH heterogeneous mice. Trace amounts of Neu5Gc were also detected in the tissues of CMAH null homogeneous mice (CMAH–/−) and in human blood serum using ESI-ToF-MS, suggesting its presence may be linked to dietary intake of Neu5Gc-containing foods, as Neu5Gc cannot be synthesized endogenously in CMAH–/– mice, and in humans. The DAPMI-ITag and the labeling technology developed in this study significantly improve the sensitivity of Sias detection compared to conventional tags such as o-phenylenediamine (OPD), and provide a new chemical tool for the exploration of Sias’ biological roles and their use as biomarkers in different human conditions.

Congratulations to the team @BristolChem and collaborators in China #Voglmeir's team & Spain @MattiaGhirarde2 for the fantastic team effort on developing a new Imidazolium Tag (DAPMI-ITag) for Precise Sialic Acid Detection in Human Serum and CMAH-Null Mouse Tissues pubs.acs.org/doi/10.1021/...

Marcin Czerwinski
Interesting review about glycoRNAs as immune regulators.
tinyurl.com/mvutz9ye

#glycotime

We’re thrilled to feature an incredible lineup of plenary speakers at #Glyco27!

These experts will share cutting-edge insights and the latest advancements in glycomics across areas of health, including immunology, neuroscience and more!
Register today: glyco27.org/en/begin

@glycotime.bsky.social

@glycotime.bsky.social @ozglyco.bsky.social @glycosmos.bsky.social @glygen.bsky.social @giaglyco.bsky.social
#glycoscience #glycomics #glycans #CallForAbstracts #glycobiology

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬

The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy

www.biorxiv.org/content/10.1...

Angstrom-resolution imaging of cell-surface glycans Glycobiology is rooted in the study of monosaccharides, Angstrom-sized molecules that are the building blocks of intricate glycosylation patterns. Glycosylated biomolecules form the glycocalyx, a dens...

This is an amazing achievement - angstrom scale imagine of glycan molecules, what a great time for #glycotime. Congrats Leonhard Möckl!

www.biorxiv.org/content/10.1...

Woah. Should be a nice way to validate in-solution computational models of glycans.

View from the office today 🌈
Someone needs to check the hole on the 8th green..

Don’t forget to submit your abstracts by Feb 10, 2025 @glyconet.bsky.social

📰Our January 2025 newsletter is out now!

Don’t miss news about #Glyco27, IGO Award recipients, research & training opportunities!

Read here: mailchi.mp/5547444d411c...

@glycotime.bsky.social #glycomics

🚨Call for abstracts for #Glyco27
Submit your abstract by Feb 10, 2025
Themes: Glycomics, Glycobiology of Disease, Tools to Drive Glycobiology, Glycans in Immunology, and Emerging Concepts in Glycans
More info: glyco27.org/en/callforab...

@glycotime.bsky.social #glycomics #Abstracts

Are you affected by the Trump administration’s pause on health communications, science meetings, and reviews? Have you been affected by the Trump administration’s pause on communications, science meetings, and reviews? @statnews would like to hear from you.

Please repost! We’re trying hard to sort out rumors from truth + would like to hear from anyone affected by the new federal orders + NIH pause. Patients, scientists, health equity researchers: please contact us below. We won’t share anything without your permission. www.statnews.com/2025/01/25/t...

Mannose metabolism reshapes T cell differentiation to enhance anti-tumor immunity Qiu et al. identify that reduced mannose metabolism is a key characteristic of T cell dysfunction. Enhancing mannose metabolism in T cells through D-mannose supplementation boosts anti-tumor activity, limits exhaustion differentiation, and maintains T cell stemness. Mechanistically, D-mannose promotes the OGT-mediated O-GlcNAcylation and stabilization of β-catenin, enhancing T cell stemness.

Expanding T cells in the presence of mannose can generate T cells with progenitor like properties www.cell.com/cancer-cell/...

Production of site-specific antibody conjugates using metabolic glycoengineering and novel Fc glycovariants Molecular conjugation to antibodies has emerged as a growing strategy to combine the mechanistic activities of the attached molecule with the specificity of antibodies. A variety of technologies have been applied for molecular conjugation; however, these approaches face several limitations, including disruption of antibody structure, destabilization of the antibody, and/or heterogeneous conjugation patterns. Collectively, these challenges lead to reduced yield, purity, and function of conjugated antibodies.

New twist on leveraging modified antibody glycans for site specific bioorthogonal ADC conjugation, featuring #proudofalumni Kevin Yarema and coworkers

www.jbc.org/article/S002...

Guardians at the Gate: Optimization of Small Molecule Entry Inhibitors of Ebola and Marburg Viruses Ebola and Marburg (EBOV and MARV) filoviral infections lead to fatal hemorrhagic fevers and have caused over 30 outbreaks in the last 50 years. Currently, there are no FDA-approved small molecule therapeutics for effectively treating filoviral diseases. To address this unmet medical need, we have conducted a systematic structural optimization of an early lead compound, N-(4-(4-methylpiperidin-1-yl)-3-(trifluoromethyl)phenyl)-4-(morpholinomethyl)benzamide (1), borne from our previously reported hit-to-lead effort. This secondary round of structure–activity relationship (SAR) involved the design and synthesis of several deconstructed and reconstructed analogs of compound 1, which were then tested against pseudotyped EBOV and MARV. The antiviral activities of the most promising leads were further validated in infectious assays. The optimized analogs exhibited desirable antiviral activity against different ebolaviruses and reduced off-target activity. Additionally, they also possessed druglike properties, that make them ideal candidates for in vivo efficacy studies as part of our ongoing drug discovery campaign against EBOV and MARV.

Interesting design of new ebola/marburg virus inhibitors pubs.acs.org/doi/10.1021/...

Carbohydrate-mediated interactions between chloroviruses and the immune system - Communications Biology This study explores how viral N-linked glycans play a role in the interaction between chloroviruses glycoproteins and key receptors of the innate immune system.

"Collectively, these results demonstrate the ability of the innate and adaptive immune systems to recognize chlorovirus glycoproteins, a process dependent on their specific N-glycan structures..."

Our #glycotime study just published @commsbio.bsky.social

www.nature.com/articles/s42...

Palleon Pharmaceuticals and Henlius Collaborate to Advance Glycan Editing as a Treatment for Autoimmune Diseases Palleon Pharmaceuticals, a company pioneering glyco-immunology drug development to treat autoimmune diseases and cancer, today announced a collaborati

Exciting news from Palleon Pharma. Engineered sialidases remove glyco-immune checkpoint ligands from B cells, thereby potentiating antibody-mediated B cell depletion for treatment of autoimmune disease. Clinical studies gearing up in partnership with Henlius Biotech #glycotime

Door to the Stark Lab at MIT decorated for the holidays

Happy holidays from our team to yours ☃️
Looking forward to lots more #glycotime in 2025!
@mitdeptofbe.bsky.social

Reading this more, this has the potential to be one of the most promising tools for glycoscience.

We all know PROTACs (bifunctional molecules that bind to a protein of interest and an E3 ubiquitin ligase), imagine the same thing but for glycans (GLYTAC).

Just need to fine-tune the selectivity.

Advertisement for the European Glycoscience Webinar on November 21st at 16h CET. The slide features profile photos on the left-hand side of the two speakers, Dr. Giorgia Greter and Prof. Lara Mahal, as well as their webinar titles and institute logos on the right-hand side.

Love #GlycoTime? 💕

Looking forward to chair this afternoon's exciting EGC session featuring:
🦠 Dr. Giorgia Greter @ Swiss biotech start-up Baxiva
🏥 Prof. Lara Mahal @ University of Alberta

Registration is still open!
Link: unibas.zoom.us/webinar/regi...

GlyCombo Enables Rapid, Complete Glycan Composition Identification across Diverse Glycomic Sample Types Glycans are sugar-based polymers found to modify biomolecules, including lipids and proteins, as well as occur unconjugated as free polysaccharides. Due to their ubiquitous cellular presentation, glyc...

Our best example so far is GlyCombo, our free, open-source software which streamlines the first daunting step of LC-MS data analysis, getting a candidate list of detected glycans.
It's fast and complete, improving throughput and enabling discoveries.
pubs.acs.org/doi/10.1021/...

I'm delighted to share that I will be joining the Department of Microbiology at Harvard Medical School in January 2025. The Laboratory of Systems Virology will study the dark proteome of viral genomes to understand how viruses work their magic🪄 Thrilled to be back in beautiful Boston 🌟

This one is for the #glycotime community: has anyone tried to cleave Lewis X/CD15 from the surface a live mammalian cells using a recombinant fucosidase? I found single paper from 2007 but curious if anyone else has a recent protocol to share 😬

Still time to register to awsome Glycobiology Gordon Research Conference 2025. We have a fantastic line of speakers and this will be top #glycotime in Tuscany !! Register at www.grc.org/glycobiology...

New #glycotime book just came out:

www.cambridgescholars.com/product/978-...