High-speed AFM imaging of the Entropic Disassembly of DNA Origami for Single-Molecule Biosensing Circulating microRNAs (miRNAs) are promising biomarkers for disease diagnosis, but their small size and instability hinder direct detection. The detection of small RNA, such as miRNA, using solid-stat...

You can read the preprint here www.biorxiv.org/content/10.1...

We designed a DNA origami system that disassembles in presence of specific miRNA and visualised the disassembly process using high speed AFM. Find a link to the preprint in the comments. Feedback and comments are highly appreciated!!!

Exciting work led by @nano-chalmers.bsky.social delivered in collaboration with @georgerheath.bsky.social introducing a rationally designed DNA origami system for the multiplexed detection of miRNA in crude RNA extracts via nanopore sensing.

Specialized ribosomes: integrating new insights and current challenges | Philosophical Transactions of the Royal Society B: Biological Sciences Variation in the composition of different ribosomes, termed ribosome heterogeneity, is a now well established phenomenon. However, the functional implications of this heterogeneity on the regulation of protein synthesis are only now beginning to be ...

❗NEW PAPER❗

Check out our review on #ribosome heterogeneity and specialisation published today!

We discuss the mechanisms of specialisation, review the latest research and explore how technological advancements will drive the next set of research questions.

doi.org/10.1098/rstb.2023.0377 (1/2)

amazing!!!! i loved reading your first paper and looking forward to reading (and using) this one!!!!

dream team reunited!!!!

Super exciting new paper from three of our brilliant RiboCoders and Nikki Weckman exploring the use of solid-state nanopore in monitoring enzyme digestion pubs.acs.org/doi/10.1021/...

Congrats @nano-chalmers.bsky.social @paoloactis.bsky.social and Emma Thomson!

Artwork inspired by Joy Division 👌

Reposted linking @nano-chalmers.bsky.social who led, designed and delivered this work !!!

All credit goes to Chalmers Chau who designed and delivered this work and to @ribocode.bsky.social for supporting us.
@chancao.bsky.social Have a great time in Bolzano #SMPS4

Working with Nikki Weckman (U toronto) and Emma Thomson (U Sheffield) 2e found out that a single point mutation in the guide RNA of the Cas9-RNP complex is sufficient to alter the digestion activities.

We use a polymer electrolyte as trans electrolyte which allows us to use biochemical buffers without compromising the detection sensitivity so we can monitor in real time enzymatic reactions

The TOC graphic was inspired from the signal analysis of the CP 1919 pulsar and Joy Division's Unknown Pleasures album. @ceesdekker.bsky.social ever publish a na-na-na nanopore album we have already got the cover sorted ! (those waves are DNA molecules translocating through the nanopore)

Solid-State Nanopore Real-Time Assay for Monitoring Cas9 Endonuclease Reactivity The field of nanopore sensing is now moving beyond nucleic acid sequencing. An exciting avenue is the use of nanopore platforms for the monitoring of biochemical reactions. Biological nanopores have b...

We cannot join #SMPS4 but hot off the press our nanopore work to monitor CAS9 reactivity and its off-target effects. pubs.acs.org/doi/10.1021/...

Job Opportunity at University of Leeds: Senior Research Technician in Gene Expression Are you an experienced technician looking for a challenge?  Do you have a background in gene expression and RNA biology?  Do you have experience in using model organisms? Do you want to further your c...

Come and join RiboCode!

Are you interested in understanding the mechanisms of mRNA translation? Do you have experience using D. melanogaster? Would you like to join an interdisciplinary team?

If so, apply for our Senior Research Technician role @universityofleeds.bsky.social

shorturl.at/ytnrA