New OA Article: "Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis" rdcu.be/feoNF

Lipid-driven mechanism for immune activation by a bacterial lipopeptide. Perception at the cell membrane leads to deformation, mechanosensing and early signalling. #PlantScience

Phytophthora targets plant extracellular vesicles to promote infection a and b, TET8, TET9, PEN1, but not ARA6 could be detected in total extracellular vesicles (P100 pellet) of Arabidopsis. a, eGFP labeled proteins and plant actin were detected by western blotting. b, Confocal microscopy of EVs isolated from TET8-eGFP, TET9-eGFP, ARA6-eGFP and eGFP-PEN1 expressing Arabidopsis respectively. Bar, 10 µM. c, EVs were isolated from TET8-eGFP expressing lines. AWF (without EVs) were prepared by ultracentrifugation at 100,000 × g for 6 hours. Proteins were detected by western blotting. d, Schematic representation of purification of plant EVs by Size Exclusion Chromatography (SEC). e, Elution fractions of Size Exclusion Chromatography were collected. TET8-eGFP, TET9-eGFP and eGFP-PEN1 were detected by western blotting. Dashed box indicates collection of EV fractions for further analysis. BV (Buffer Volum). f, Transmission electron microscope (TEM) observation of EVs fraction (Elution: 0.496-0.540 ml). Bars, 200 nm. g, Nanoparticle tracking analysis (NTA) of EVs fraction (Elution: 0.496-0.540 ml) was collected. Graph shows distribution of particle size intensity. h, SEC-EV particle number corresponding to different protein concentrations. EV abundance was quantified by NTA, with particle count representing EV quantity. i, TEM observation of soybean EVs (Size Exclusion Chromatography purification, same fractions with Arabidopsis EVs). EVs from soybean leaves were isolated. j...

Phytophthora targets plant extracellular vesicles to promote infection
->Nature | More on "Plant pathogen extracellular vesicle infection" at BigEarthData.ai | #Infection

GitHub - RILAB/statements: Successful Job Applications and Grants Successful Job Applications and Grants. Contribute to RILAB/statements development by creating an account on GitHub.

That time of year again! If you'd like an example of job apps in academia or industry, here's a good resource. If you got a job in industry or academia in the last few years, please consider adding yours! github.com/RILAB/statem...

Pathogen-inducible expression of autoactive NLRs confers multi-pathogen resistance in tomato

www.biorxiv.org/content/10.1...

AlphaFold 3 captures oligomeric states and interaction dynamics of MLO ion channels www.biorxiv.org/content/10.64898/2026.04...

Specialised root hair cells facilitate rhizobial infection www.biorxiv.org/content/10.64898/2026.04...

Lipid-trap mass spectrometry identifies lipid–protein interactions in cells - Nature Cell Biology Paquola et al. present a technique named lipid-trap mass spectrometry (LTMS) to study lipid–protein interactions directly captured from mammalian cells.

Lipid-trap mass spectrometry identifies lipid–protein interactions in cells
www.nature.com/articles/s41...

Visualizing suborganellar lipid distribution using correlative light and electron microscopy - Nature Cell Biology Lennartz et al. introduce a correlative light and electron microscopy workflow, Lipid-CLEM, combining near-native lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively a...

☕ @mathilda95.bsky.social @nadlerlab.bsky.social & co introduce a correlative light and electron #microscopy workflow, Lipid-CLEM, combining near-native #lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively analyses lipids in membrane nanodomains.
bit.ly/4moYLJH

Cool story and like how your drawings come together. Congratulations to all of you! 🥂

Preprint alert 🚨 (1/10) 🧵

𝘈𝘶𝘵𝘰𝘱𝘩𝘢𝘨𝘺 𝘪𝘴 𝘢 𝘱𝘢𝘳𝘢𝘥𝘰𝘹 𝘪𝘯 𝘱𝘭𝘢𝘯𝘵 𝘪𝘮𝘮𝘶𝘯𝘪𝘵𝘺:
It can promote resistance, susceptibility, or restrict cell death 🤯

So what is its actual role?

We show it acts as a spatial organizer of immunity across cell types 🌱🦠 #proteostasis

www.biorxiv.org/content/10.6...

CypA1 ensures the process of rhizobial intracellular infection.

Cyclophilin A-mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in #legumes

Takashi Goto, et al.

nph.onlinelibrary.wiley.com/doi/10.1111/...

#PlantScience

Fig. 1 Proposed mechanism of benzoxazinoid (BX) transfer from BX-producing rye plants (Secale cereale) to clover plants (Trifolium repens) in live, arable soil.

Transferred resistance – interspecific transfer of plant defence against soilborne pathogens

📖 nph.onlinelibrary.wiley.com/doi/10.1111/...

👆 A #Commentary by Costa & Mathesius on this article by Hama et al.
👇

📖 nph.onlinelibrary.wiley.com/doi/10.1111/...

#LatestIssue

LipoTag: A minimal motif for live and functional imaging of plant cell membranes. www.biorxiv.org/content/10.64898/2026.04...

New preprint out! 🚀
biorxiv.org/content/10.6...

We mapped how septins organise infection in the rice blast fungus—and found they are far more dynamic than previously thought.

Thread 👇

#FungalPathogenesis #CellBiology #septins

Women in plant science around the world The article series ‘Women in plant science around the world’ marks the 30th anniversary of Trends in Plant Science, a milestone that invites us to reflect on three decades of shared discoveries, international collaborations, and the diverse voices that propel the field forward. By centring 30 of our women authors from 30 countries, we honour the global community that continues to advance plant science, and we hope to inspire the next generation of women researchers, as well as researchers from other still underrepresented groups in plant science.

Women in plant science around the world #plantscience

Spatial organization of plant defense at the infection front www.biorxiv.org/content/10.64898/2026.04...

Fusarium graminearum Fusarium graminearum is a widespread pathogen, responsible for head blight (also known as ear blight) and crown rot of wheat and barley, ear rot of corn, root rot of soybean, as well as various diseases of oats, rye, rice, millet, quinoa, hemp, and others. Infection can cause significant reductions in both yield quantity and grain quality. F. graminearum also produces multiple specialized metabolites with high biological activity, some of which are toxins or hormone mimics. The most well-known of these metabolites is deoxynivalenol (DON, belonging to the trichothecene family of mycotoxins), a virulence factor that interacts with ribosomes and interferes with protein synthesis.

Fusarium graminearum

Acute remodeling of phosphoinositide lipids promotes endocytosis downstream of RALF/FERONIA signaling Serre et al. reveal that RALF peptides rapidly trigger PI4P-to-PI(4,5)P2 conversion at the plasma membrane downstream of the receptor kinase FERONIA. This acute phosphoinositide remodeling, mediated b...

Acute remodeling of phosphoinositide lipids promotes endocytosis downstream of RALF/FERONIA signaling: Current Biology www.cell.com/current-biol...

Thank you Şuayb 😊

a group of people are dancing in a hallway and the word team work is visible ALT: a group of people are dancing in a hallway and the word team work is visible

10/10 As so often, this has only been possible as a great team effort. So shoutout to my former PI @dromius.bsky.social, @matt-macleod.bsky.social, @marioncmueller.bsky.social, Issac Coven, Edouard Evangelisti, Inma Hernandez-Pinzon and Matthew Moscou and everyone else that helped make this happen.

ALT:

🧵9/10 Why do hvscar-b,c roots become more resistant against the pathogen but more susceptible to the symbiont? …would I love to know that! So fingers crossed🤞 that I can acquire funding this year to delve deeper into the molecular mechanism leading to SCAR susceptibility functions.

a man in a tie is sitting at a table with his mouth open . ALT: a man in a tie is sitting at a table with his mouth open .

🧵8/10 Why is this exciting:
It shows that HvSCAR-B and HvSCAR-C removal: 1)boosts resistance to a root-infecting pathogen 2) does not impair beneficial mycorrhiza. 3) Under lab conditions, normal mutant growth 4) The susceptibility function works though a mechanism shared across monocots and dicots

🧵7/10 Jackpot: We found less of the pathogen inside the hvscar-b,c double mutant roots. And to our surprise, when we tested the roots for colonization with a symbiotic fungus (F. mosseae), we observed more of the symbiont inside the roots.

🧵6/10 Since HvSCAR-B and -C were both able to rescue api, we generated a double mutant to see if they work redundantly. And indeed, the hvscar-b,c mutant had shorter root hairs, similar to api. However, seed production was not harmed. So what happens if we infect the roots of this mutant?

🧵5/10 At first glance not much: initially HvSCAR CRISPR single mutants grew normally. BUT before harvest we noticed a striking difference: hvscar-a plants produced fewer seeds. However,hvscar-b and -c seed numbers were still comparable to the WT. None of the mutants had shorter root hairs like api.

two men making a peace sign with their fingers ALT: two men making a peace sign with their fingers

🧵4/10 We identified three SCAR genes in barley and tested whether they have functions similar to API. Two of the genes, namely HvSCAR-B and HvSCAR-C, are closer related to API and can rescue the api short root hair phenotype. But what happens if we remove these genes from barley (CRISPR)?

ALT:

🧵3/10 One such candidate: The Medicago SCAR gene API. api mutants show increased resistance to Phytophthora palmivora root infections but beside of shorter root hairs, develop quite normal in the lab. So we asked: Can we transfer the resistance principle from a dicot into a monocot crop species? 🌾

ALT:

🧵2/10 Microbes rely on susceptibility (S) factors (plant genes) to successfully infect their hosts. Removing S-genes can provide durable and broad resistance. The challenge for resistance engineering: finding those that can be removed without harming plant growth or yield ⚠️

🧵1/X 🚨New Preprint🚨 Root pathogens are hard to manage, and we know little about plant genes enabling infection (susceptibility genes). Here, we show that SCARs are susceptibility factors in barley roots - and their loss affects pathogens/symbionts in distinct ways www.biorxiv.org/content/10.6...

We have been cooking up this story for a while and we are excited to finally be able to share!

Read on if you're interested in whole plant regeneration WITHOUT the application of hormones!