Bacteriophage data on capsular Klebsiella bacteria barcoded library

New paper 🔔

Collaboration
@sullivan-lab.bsky.social
Marissa Gittrich led the work

Klebsiella phage biology has focused almost entirely on capsulated strains, but capsule loss is the #1 resistance mechanism under phage predation. We used a naturally acapsular host to reveal the receptor landscape

🎉🥳 Preprint out 🥳🎉

Here is a work you will hear about for a while, lead by @lucasmoriniere.bsky.social in the @vivekmutalik.bsky.social's lab, we developed a workflow predicting phages receptor specifity and identifies RBP from genomes🤯

Very happy to be part of this adventure and this dream team 🥰

Phage receptor prediction from genome sequencing alone. Bacterial receptor (blue) interacting with phage proteins (purple) is shown here

📣Huge preprint 🔔
Today we share something our group has been working toward for a long time, led by @lucasmoriniere.bsky.social We asked can we predict which receptor a phage targets from its genome sequence alone? For most phages, we couldn’t. So Lucas set out to do something I had only dreamed of.

Postdoc - Microbiome - Innovative Genomics Institute University of California, Berkeley is hiring. Apply now!

Hey #microsky #microbiome nerds!

My collaborator Spencer Diamond is hiring a postdoc. You get to work with a lot of awesome microbiome data, including rumen microbiome data. Spencer's a great guy to work with and has taught me a lot.

aprecruit.berkeley.edu/JPF05234

Identifying Phage Host Receptors Using TraDIS The growing antimicrobial resistance crisis has sparked renewed interest in using bacteriophage (phage) as a treatment for antibiotic-resistant infections. Phage are viruses that infect and often kill...

One more paper about phage-host interaction!

www.biorxiv.org/content/10.1...

#microsky #phagesky

The host range of generalist and specialist phages in capsule-diverse Klebsiella hosts is driven by the evolvability of receptor-binding proteins Capsule diversity limits phage host range by affecting RBP interactions in capsulated bacteria. This study shows that generalist phages evolve host range through RBP mutations and recombination, while...

The host range of generalist and specialist phages in capsule-diverse Klebsiella hosts is driven by the evolvability of receptor-binding proteins

@plosbiology.org from @pilardomingoc.bsky.social

journals.plos.org/plosbiology/...

#microsky 🎊🎉

High resolution lineage tracking tech from Robin & Adam D team from Berkeley! 🙌

We have used this for phage-host interaction work too in the forthcoming preprint.

Bacteriophage genome-wide transposon mutagenesis Bacteriophages have genomes that span a wide size range, are densely packed with coding sequences, and frequently encode genes of unknown function. Classical forward genetics has defined essential gen...

Awesome work from the JBD lab! Genome-wide mutagenesis of phages is so interesting and helpful!

www.biorxiv.org/content/10.1...

Hey phage lovers,

What do you think of the phage-fantasy paintings I've made?

Wow! Terrific work!

📣 New preprint from phagefoundry.org team 📣

What are the phage-host interactions at a strain-level scale?
Here Avery (Machine Learning) and Lucas (Experimental validation), share their new workflow to predict these interactions!

I am very very grateful to work with these talented scientists!

What a nice paper!

Cryo-EM structure of bacteriophage Bas63 reveals structural conservation and diversity in the Felixounavirus genus Cryo-EM of phage Bas63 reveals unique capsid and tail features, offering insights into Felixounavirus evolution and architecture.

www.science.org/doi/10.1126/...

Beautiful paper describing the structure of E.coli bacteriophage Bas63!

Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes - Nature Biotechnology Ribonucleoproteins, nucleic acids and proteins are delivered into target cell types with nanosyringes.

Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes - @zhangf.bsky.social go.nature.com/46OFcoj

😰

This is really heartbreaking. So many, so many ppl suffering. 😢

😤

NSF stopping salaries of postdocs and researchers is outrageous to say the least.

If you are in the USA, & want to do something worthwhile,

call your elected official.

This is damn 🚨 urgent.

Do it today.

You should go to Muir woods.

Experimental horizontal transfer of phage-derived genes to Drosophila confers innate immunity to parasitoids Metazoan parasites have played a major role in shaping innate immunity in animals. Insect hosts and parasitoid wasps are excellent models for illumina…

Excited to share the major work of my dissertation, out now in @currentbiology.bsky.social! We use Drosophila genetics to dissect the evolution of phage-derived toxins that were horizontally transferred to insects and now function as potent anti-parasitoid defenses (1/n): tinyurl.com/2k9ypyxr

Celebrating 🎉 holidays!