We just received funding for a 2yr postdoc position on cryoEM of fungal gpcrs. Official call will open soon.

Contact me if you‘re interested!

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬

Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

Thanks for the fun collaboration into this fascinating “oil eating” biomachine. I learned a lot! 🧪 #TeamTomo

It was an amazing collaboration - I also learned a lot! Huge thanks to Wojciech @wojwie.bsky.social and Ricardo @lifeonthewedge.bsky.social for teaching/guiding us during the early stages of the project. Looking forward to future collaborations!

1/ Excited to share our preprint! 🥳

Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine

www.biorxiv.org/content/10.6...

This was a true team effort! Very proud of this work!

Huge shoutout to my fellow co–first authors Lena Appel, Tomas @tomaspascoa.bsky.social, Davide @tamb-o.bsky.social
Grateful to Ben @cellarchlab.com, Jan @schullerjm.bsky.social, and Matthias for leading and shaping the project throughout!

How do “LEGO-like” electron-bifurcating modules combine to drive degradation in BTEX-contaminated ecosystems?

Check out our latest preprint, where we use cryo-EM and cryo-ET to reveal how the 1 MDa BCRII complex powers extremely endergonic aromatic ring reduction.
www.biorxiv.org/content/10.6...

New preprint alert 🚨:

Who actually drives methane production in anoxic sediments 🧬🔥?

We point to a transcriptionally dominant 🦠 clade: VadinHA17.

shorturl.at/Rijc0

@bledinadede.bsky.social
@taylorpriest.bsky.social

Breathing both ways: simultaneous aerobic–anaerobic respiration in microbes Microorganisms have historically been classified as obligate aerobes or anaerobes, facultative anaerobes, or microaerophiles, reflecting differences in respiratory strategies dictated by the use of oxygen and alternative electron acceptors. Recent discoveries provide evidence that a deviant strategy, the concurrent reduction of oxygen and other electron acceptors, is more widespread than previously thought. Such co-respiring bacteria employ hybrid metabolic strategies that extend models of electron acceptor use. In this review, we investigate mechanisms of co-respiration, summarize the biochemical components enabling parallel electron flow, and discuss the regulation of aerobic and anaerobic pathways under changing redox conditions. We also examine the evolutionary context of these strategies during the rise of oxygen on early Earth and outline experimental approaches needed to resolve co-respiration in individual cells.

Breathing both ways: simultaneous aerobic–anaerobic respiration in microbes

65 years after Peter Mitchell’s chemiosmotic theory, we report an association between ATP synthase and the electron transport chain. In the preprint led by @longzhou88.bsky.social, we describe a large supercomplex and show how the supramolecular organization contribute to oxidative phosphorylation 🧵

Highly recommended! Very cool science and great projects!

Check out the latest work on PSI:Cytc6 complex from Chlamy! many congratulations @gyanaprakash.bsky.social and @schullerjm.bsky.social #cryoem

I am truly honoured and delighted to receive the GBM #PhDAward from @gbmev.bsky.social
Grateful to @schullerjm.bsky.social and Prof. Volker Müller for their continuous support and guidance!

www.uni-marburg.de/en/prfolder-...

Thank you very much, Sven!

Thank you so much! ☺️ thank you for all your support along they way!

Thank you so much, Gyana! 😊

Carbonic anhydrase catalyses the interconversion of CO2 and HCO3- and helps increasing the net-CO2 concentration inside cells. Why? CO2-reducing enzymes like RuBisCO are notoriously slow and ineffective. However, import of HCO3- is typically coupled to ATP consumption...

This is a proud and exciting moment for me. Manon continues to redefine our understanding of pyrenoid biology, as she pushes the frontiers of #TeamTomo straight into the natural world around us 🌍🌊🌾🔬

Her new group at @gmivienna.bsky.social will explore fresh and impactful questions. Check it out! 🧪👩‍🔬

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Stunning video installation by Isabell Bullerschen featuring our T. kivui #CryoET data 🧡

On view until April 19th at the HEK in #Basel!

hek.ch/en/program/e...

Mathematisch-Naturwissenschaftliche Fakultät Heute ernannte Rektorin Prof. Dr. Anja Steinbeck den Biotechnologen Dr. Johannes Rebelein zum W3-Professor für Nachhaltige Biotechnologie. Rebelein wird damit auch Direktor des Instituts für Molekular...

A childhood dream has come true – I was appointed Full Professor for Sustainable Biotechnology at the University of Düsseldorf @hhu.de : tinyurl.com/f43s74hp and as Director of the Institute for Molecular Enzyme Technology (IMET, www.iet.uni-duesseldorf.de/en/) at FZ Jülich @fz-juelich.de .

Happy to share our new paper on proton transfer in metalloenzymes, out now in @pccp.rsc.org!
Exploiting crystallography, electrochemistry, FTIR spectroscopy, and QM calculations we identified a Zundel ion in the catalytic proton tranfer pathway of [FeFe]-hydrogenase.
doi.org/10.1039/D5CP...

New preprint by @alok-bharadwaj.bsky.social explaining the workings of SURFER, a simple tool to segment membrane, micelle or nanodisc densities in cryoEM maps.

Try it via the @chimerax.ucsf.edu ToolShed:

cxtoolshed.rbvi.ucsf.edu/apps/chimera...

Despite LocScale in the name, it works on any map.

Shades of pink we picked for HDCR core 😁 was beautiful!

At long last, the Chlamy chloroplast ribosome hat 🎩 (now renamed the arm 💪) is here to say hello👋! We first spotted this extended structure in green algae a decade ago, and @florentwaltz.bsky.social did beautiful work bringing it into focus! #CryoEM ❄️ #TeamTomo 🔬 #PlantScience 🌾 #ProtistsOnSky 🧪

This is what I call a booster 🚀: Milton and colleagues use a novel, bipyridium-type redox mediator to increase the electrochemical H2 evolution activity of [FeFe]-hydrogenase by a factor of 10 compared to previous protocols.
pubs.acs.org/doi/full/10....

An incredibly challenging single particle cryo-EM structure. Capturing both the N and C termini was difficult enough, but the project required a major effort to resolve the full length protein structure to capture the overall architecture of the KICSTOR-GATOR1 complex.

Well worth it in the end!

I am very excited to share our new preprint - "A Zundel Ion in the Catalytic Proton Transfer Pathway of [FeFe]-Hydrogenase" - for which we revisited an older theory of ours, and came to surprising conclusions... 🧵
doi.org/10.26434/che...

Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...

Our latest work on the nitrogenase-like methylthio-alkane reductase, which specifically reduces reduces carbon-sulfide bonds is now out @natcatal.nature.com: doi.org/10.1038/s419.... We find for the first time large #nitrogenase metalloclusters (P- and L-cluster) outside nitrogenases.

Great work from the lab of @rebeleinlab.bsky.social ! Now out in @natcatal.nature.com