GATHeR: Graph-based Accurate Tool for Immunoglobulin HEavy- and Light-chain Reconstruction Recovering full-length, paired B-cell receptor (BCR) sequences from scRNA-seq reads remains difficult, especially in naive and memory B cells where immunoglobulin transcripts are sparse. Current metho...

New BCR reconstruction tool for scRNA-seq: assembles full heavy & light chains and annotates constant regions. Check it out:
www.biorxiv.org/content/10.1...

#scRNAseq #immunology

GATHeR: Graph-based Accurate Tool for Immunoglobulin HEavy- and Light-chain Reconstruction Recovering full-length, paired B-cell receptor (BCR) sequences from scRNA-seq reads remains difficult, especially in naive and memory B cells where immunoglobulin transcripts are sparse. Current metho...

Interested in BCR analysis, including paired heavy-light chain and constant region?👀 Check out our new tool for reconstructing BCRs from scRNAseq! Compatible with 10x and Smart-seq🧬
www.biorxiv.org/content/10.1...

*constant*ly amazed 🧬

Ultra-long sequencing for contiguous haplotype resolution of the human immunoglobulin heavy chain locus Genetic diversity within the human immunoglobulin heavy chain (IGH) locus influences the expressed antibody repertoire and susceptibility to infectious and autoimmune diseases. However, repetitive seq...

Check out long-read sequencing results in human IGH! HUGE duplications in constant gene regions...and 7 copies of IGHV3-23 on one haplotype! @lossiusandreas.bsky.social @williamlees.bsky.social

www.biorxiv.org/content/10.1...

Our collaborative paper on #Nanopore sequencing is now available on #BioRxiv. Among several new variants, we characterize a large duplication in the IGHC region, suggesting non-canonical class switching between the duplicated isotypes in #Bcells.
www.biorxiv.org/content/10.1...