Tardigrades resist heavy DNA damage by actively repairing such lesions & expressing DNA-protective proteins. Yet, they are not immortal. How & why do they die? @morielab.bsky.social now shows that their Achilles' heel is DNA replication: duplicating your DNA... will kill you doi.org/10.1111/febs...
6-way multiplexing at the first step allows 10-20 libraries to be made in parallel
Great work Neesha and thanks to our collaborators and our funders
TrAEL-seq read density reflects the speed or replication forks, allowing quantification of interactions with genomic elements such as transcription
TrAEL-seq profiles rival quality to OK-seq but are much easier to generate
Need to profile DNA replication genome-wide?
TrAEL-seq reveals replication patterns and fork progression in unsynchronised, unlabelled and unsorted cells
Our revised protocol:
• Provides far better data from mammalian cells
• Includes 6-way multiplexing
academic.oup.com/nar/article/...
Exciting new project in our lab @babrahaminst.bsky.social! We are working with @alesdantas.bsky.social at @newcastleuni.bsky.social to understand how extrachromosomal circular DNA mediates drug resistance in fungal pathogens
Funded by @wellcometrust.bsky.social
#ecDNA #eccDNA #Candida #Zymoseptoria
Post-doc job alert!
Only a week left to apply for this post doc role, joining our team at the Babraham Institute @babrahaminst.bsky.social to explore ageing metabolism and epigenetics.
babrahaminstitute.livevacancies.co.uk#/job/details...
Post-doc job alert!
We have an exciting opportunity for a post doc to join our team at the Babraham Institute, exploring ageing metabolism and epigenetics.
babrahaminstitute.livevacancies.co.uk#/job/details...
6-way multiplexing at the first step allows 10-20 libraries to be made in parallel
Great work Neesha and thanks to our collaborators and our funders
TrAEL-seq read density reflects the speed or replication forks, allowing quantification of interactions with genomic elements such as transcription
Our new Preprint describes an improved TrAEL-seq protocol:
• Provides far better data from mammalian cells
• Includes multiplexing for higher throughput
TrAEL-seq reveals replication patterns and fork progression in unsynchronised, unlabelled and unsorted cells
www.biorxiv.org/content/10.1...
If you're at the AGE meeting don't miss the BSRA Korenchevsky Award talk from Hanane Hadj-Moussa!
Hanane's work shows that we can acheive excellent healthy ageing through metabolic rewiring, even on an unrestricted diet
Profile:
whova.com/embedded/spe...
Preprint:
www.biorxiv.org/content/10.1...
Great work lead by Hanane Hadj-Moussa and Megan Ulusan
Babraham Institute @babrahaminst.bsky.social
#BBSRC @ukri.org
AMPK orchestrates responses to low nutrient availability from yeast to humans…
…including regulation of fatty acid synthesis through phosphorylation of the same enzyme
This highly conserved regulatory system presents a promising and unexplored target for healthy ageing research across Eukaryotes
Controlling Acetyl coenzyme A availability underlies all these effects
Acetyl coenzyme A is the building block for fatty acids but excess acetyl coenzyme A availability is detrimental
A2A cells make the fatty acid they need but get rid of excess acetyl coenzyme A into mitochondria
but AMPK activation turns off fatty acid production, which is a problem
Our A2A mutant combines AMPK activation with a mutation that allows continued fatty acid synthesis
The majority of A2A cells maintain fitness very late in life even though they don't live any longer
AMPK activity is widely considered to be beneficial for ageing health
We show that AMPK activation in budding yeast allows some cells to age without senescence on a normal diet...
Is healthy ageing possible on a normal diet?
Our latest Preprint describes metabolic re-engineering of yeast to avoid senescence
The A2A mutant we created is super-fit late in life even on a rich high glucose diet
www.biorxiv.org/content/10.1...
Congratulations to our PhD student Amy Wolstenholme for winning the Poster Prize at the EMBL Drug Resistance meeting #EESDrugResistance this week
Amy's poster showed that circular DNAs providing copper resistance can be stably transmitted for hundreds of generations in yeast