@libdrstats.bsky.social videos and blog posts continue to be a great resource for me as I delve into snRNAseq analyses, and for lots of other bioinformatics-related topics! Learn more here: research.libd.org/rstatsclub/
![[2026-02-27] Strategy to drop doublets during snRNAseq QC steps](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:wzibqpwixf6x3m7faeiii7pn/bafkreihrz2opt3zv227woo4j62uq5ug5cgguylcdswxauq5ttwj2emjlta)
Check out my first @libdrstats.bsky.social presentation on one strategy our team @lcolladotor.bsky.social at @lieberinstitute.bsky.social is taking to deal with doublets during quality control for single nucleus RNA-sequencing data:
www.youtube.com/watch?v=SMA-...
I defended my PhD 10 years ago today. That was the least remarkable thing that happened that day. Sharing something I wrote about it last year. Since then "the horrors persist but so do we." And with dignity.
Great work from Claire, Ravi, and the whole team– the initial lifelong continuous screen of behaving vertebrates provided a wealth of ideas on progression/staging of brain function across lifespan. Behavior is unmatched as a noninvasive quantitative readout of brain function (and, one that matters).
Today I received a note from a grad student who lives in Tehran. Her note gives you firsthand experience of what it’s like to live in a city that is being bombed, and what it’s like to be young and feel despair about your future.
rezashadmehr.blogspot.com/2026/03/hope...
😍 looks like a lovely time!
Super excited about these #FluorescentFriday images 🔬🧪 as part of project led by @drjennkim.bsky.social in @kr-maynard.bsky.social lab to understand the molecular anatomy of VTA-NAc circuitry in the developing human 🧠
Graph of award probability of R35 and R01 from NIH factbook as a function of review rank percentile. As is apparent, 2025 is a significant departure, with lower award probabilities at all scores <40 and significant departures from norm, where even being in the top 10% is no longer a nearly certain indicator of success. Data source: https://report.nih.gov/nihdatabook/report/302
The data is in: the NIH goalposts have shifted.
What were once almost certain fundable scores have become coin flips and what used to be likely grants have become aspirational, leading to fewer awards.
Another manifestation of how HHS policies have led to fewer awards and less science.
#FluorescenceFriday to mark my first foray into human neuroanatomy @lieberinstitute.bsky.social 🧠 RNAscope of marker genes for the ventral tegmental area (VTA) and nucleus accumbens (Ac) in the developing human brain
FIRST post here on Bluesky - we’re excited to share that our large spatial transcriptomics study of the human dlPFC in schizophrenia is now available on bioRxiv📄: www.biorxiv.org/content/10.6... (1/9)
Bad Bunny sends a message at the Super Bowl:
"God Bless America"
"The only thing more powerful than hate is love"
Honored to be appointed Chief Scientific Officer @lieberinstitute.bsky.social. LIBD is a uniquely collaborative place with extraordinary biological resources and a commitment to translation 🧠🔬🧪. Grateful to my colleagues and excited to help guide our next chapter!
www.libd.org/keri-martino...
Ollie (left, black and white dog) and Bella (right, brown and white dog) cuddling nose to nose
Happy new year from Ollie & Bella 🐾 May your 2026 be as cozy and warm as these two ❤️
“Basic neuroscience hasn’t produced new drugs.” 💊
Not true - zuranolone (PPD), suzetrigine (pain), gepants (migraine), and more... were born out of a long arc of studies in the lab.
I wrote a Perspective on why this matters. @thetransmitter.bsky.social
www.thetransmitter.org/drug-develop...
Finally on Bluesky and I’m really excited to share this latest preprint with @jorge-miranda.bsky.social in which we functionally examined the role of a medial prefrontal cortex (mPFC)- Locus Coeruleus (LC) circuit in regulating sustained attention.
1/7
www.biorxiv.org/content/10.6...
Experimental design for LIBD LFF Spatial ERC project
Excited to share our latest preprint from @lieberinstitute.bsky.social spatial + single-cell RNA-seq map the ERC & explore how AD risk shapes gene expression doi.org/10.1101/2025...
Our major finding: “APOE E4 Alzheimer’s Risk Converges on an Oligodendrocyte Subtype in the Human Entorhinal Cortex”
Excited to share new preprint led by Sergio Salas on “unassigned RNAs” in imaging-based spatial transcriptomics. Across technologies, 30-40% of transcripts fall outside segmentation, & many show structured, biologically meaningful extrasomatic patterns.
📄 www.biorxiv.org/content/10.6...
Our paper using endoscopic in vivo calcium imaging to uncover how neuronal population dynamics in the prelimbic cortex track attention and task engagement during the rodent continuous performance test (rCPT) is now online
www.nature.com/articles/s41...
Scientists point to a long list of findings that emerged out of fundamental research, the type of studies the US government is cutting, and went on to change the world. Nature lists a few of their examples. 🧪
And ofc, an unforgettable time with the best class 😎 huge thank you to all the course leaders and lecturers for the fun and incredible learning experience! 3/
Also had the opportunity to generate/analyze our own data, which was really useful to solidify all the new concepts we learned about. Some fun lab pics fr/ preparing virus, dissecting brain regions for MAPseq, brain tissue clearing, and in situ sequencing our BARseq-prepped tissue on the scope 🤩 2/
Had the most rewarding time at the HTNA #cshlcourses It was fun science-ing all day/night for 2wks to learn modern viral, whole 🧠 imaging & barcode-based seq approaches to understand neuroanatomy/function. Inspired & energized as I think of all the ways these tools can be applied in my own work!🧪 1/
Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus www.biorxiv.org/content/10.1101/2025.11....
Proud to announce our paper ‘Transcriptomic Analysis of the Human Habenula in Schizophrenia’ from @lieberinstitute.bsky.social is the cover article for the November issue of the American Journal of Psychiatry! 🧠 #HabenulaLIBD #snRNAseq #Habenula doi.org/10.1176/appi...
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
![[2025-10-16] What Makes a Good Data Visualization?](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:h7p2smbyfobkpcs7dbgvhmeh/bafkreifn2oufypnuqtxrnasijy3rebx5cz75pot37o2r2np3qp6qk6sudq)
"What Makes a Good Data Visualization?" 🤔
Check @christinezhang.bsky.social's 🤩 excellent 💯 guest lecture for 140.776 Statistical Computing at @johnshopkinssph.bsky.social
📽️: youtu.be/SeLucCb05Dk
Slides: speakerdeck.com/lcolladotor/...
#RStats @jhubiostat.bsky.social @lieberinstitute.bsky.social
Thrilled to share this work from my postdoc with Tom Carmichael, available online @ Neuron! Long story short: we find even more evidence that all reactive astrocytes are not equal, and we can take advantage of those differences to improve repair after stroke.
www.sciencedirect.com/science/arti...
Effective and empathetic scientific communication is so critical right now. We had an interactive workshop at the #2025ICDB led by @rebeccacalisi.bsky.social and Dr. Kevin Alicea-Torres and facilitated by @spiraldoc.bsky.social and myself- and we practiced this. Please check out our perspective!
All scientists can easily respond to this and you can choose to stay anonymous. I modified my comment from the FASEB response. We all know that international scientists are essential to US science and that these proposed rules are cruel to our colleagues. www.regulations.gov/comment/ICEB...
