🚀 New preprint! @jacobsenucla.bsky.social x @doudna-lab.bsky.social collaboration: High-activity TnpB (Ymu1-WFR) + a multi-gRNA system in TRV enables heritable, tissue-culture-free multiplex editing in plants. Big potential for plant biotech 🧬🌾🌱
doi.org/10.64898/202...
New preprint 👉Doudna x Bryant x Jacobsen x Savage collaboration!
Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss
We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage
www.biorxiv.org/content/10.6...
How do the ancestors of CRISPR-Cas unwind DNA and how can this lead to better genome editing? With our collaboration between @doudna-lab.bsky.social x @jacobsenucla.bsky.social x Zev Bryant's lab x @savagecatsonly.bsky.social we've uncovered the secrets behind TnpB's dynamics!
The plasmids associated with this paper are available on addgene now! www.addgene.org/browse/artic...
Welcome to the JCC family!
Our paper is finally out in Molecular Cell! 🚀 We uncover why PAM-relaxed Cas9 variants like SpRY are inefficient — they get kinetically trapped during target engagement. Mechanistic insights like this are key to engineering smarter, faster genome editors. Huge thanks to the team! #CRISPR #editing🔬🧬
Now online at Molecular Cell! What makes SpyCas9 such an efficient editor? Read more at: www.cell.com/molecular-ce...
Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!
Pictured: Kevin Wasko (left) and Honglue Shi in front of their gel imager, confirming that the SpRY Cas9 variant struggles to effectively open DNA
“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author @honglue.bsky.social of the @doudna-lab.bsky.social on his new paper out today in Molecular Cell
Read here: shorturl.at/p664D
See details in this website
gess.hms.harvard.edu/event/meirui...
🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
Catch @honglue.bsky.social at the Harvard Genome Engineering Seminar Series this Monday, April 7 at 1pm EST / 10am PST! If you’ve ever wondered why SpyCas9 is so efficient at genome editing, don’t miss his talk—Zoom link: harvard.zoom.us/j/94394339529
Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥
📍 Zoom link: harvard.zoom.us/j/94394339529
Congratulations! Such an amazing work @erinedoherty.bsky.social
Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.
Check it out:
Jennifer Doudna speaking at Stand Up for Science in Berkeley
crowd at Stand Up for Science in Berkeley
crowd at Stand Up for Science in Berkeley
crowd at Stand Up for Science in Berkeley
Thanks to out @ucberkeleyofficial.bsky.social community for joining us at Stand Up For Science! @jenniferdoudna.bsky.social spoke about how NIH funding supported her PhD & NSF funding supported her development of #CRISPR genome editing. Federal funding is needed for life-saving science!