Very grateful for all the help from our collaborators @benvh123.bsky.social, @emmabignon.bsky.social, Amy Whitaker, Alan Tomkinson, and Natacha Gillet!

Excited to share the first work from my new lab describing how LigIIIα catalyzes nick ligation within the nucleosome during the terminal step of BER/SSBR. Hard to believe the lab performed our first experiment just over a year ago!

www.biorxiv.org/content/10.6...

Check out the final version of this work, out today! @natcomms.nature.com www.nature.com/articles/s41...

Congratulations and welcome to the newest members of the #ACSBIOL Executive Committee!

@freudlab.bsky.social, @doc-jlmeier.bsky.social, @tmweaver.bsky.social, @ebraselmann.bsky.social

Check out the perspective my lab wrote with @freudlab.bsky.social. We provide our perspective on recent work describing the structural basis of chromatin-based base excision repair!

See our paper “Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1”. Using biochemistry, we show how DNA incisions by the MutLγ nuclease can lead to expansions, and how expansion is prevented by FAN1. Well done Issam and Valentina!

www.nature.com/articles/s41...

The final piece of my postdoctoral work in the @freudlab.bsky.social was recently published!

Quite amazing what this small little repair polymerase can do to fill 1-nt gaps in chromatin.

www.nature.com/articles/s41...

🔊There's still time to register for the #EMGS webinar: An Applied Genetic Toxicology Career in the Pharmaceutical Industry with Zhanna Sobol on April 8, 2025 at 12:00 PM EST!

Register Now: emgs-us.org/event/AGTCPI...

A nucleosome switch primes hepatitis B virus infection A reconstituted cccDNA platform facilitated the discovery of an early chromatinization event that promotes transcription of the hepatitis B virus (HBV) regulatory X gene. The use of a small-molecule c...

I am extremely proud to see our work about Hepatitis B now published in @cellpress.bsky.social

From basic research to identifying the key step of infection & a potential first drug for curing this devastating disease. This has been an incredible journey.🧵

www.cell.com/cell/fulltex...

We are still accepting application for the Postdoc position in my lab! We have some exciting projects at the interface of DNA repair and chromatin biology. If interested, please get in touch or apply for the position through the link below!

jobs.virginia.edu/us/en/job/R0...

Come be our colleague! @cu-bmg.bsky.social is running an open rank search for a structural biologist specializing in cryo-EM/ET. This is a great opportunity in a highly collegial and collaborative department.

Apply here: cu.taleo.net/careersectio...

Lab Technician 1 or 2 in Biochemistry & Molecular Genetics in Charlottesville, Virginia, United States of America | Research at University of Virginia Apply for Lab Technician 1 or 2 in Biochemistry & Molecular Genetics job with University of Virginia in Charlottesville, Virginia, United States of America. Research at University of Virginia

My lab at UVA is looking to hire a Lab Technician to join our team! This position is ideal for someone interested in obtaining research experience prior to graduate and/or medical school.

jobs.virginia.edu/us/en/job/UO...

It is still possible to apply to this postdoc position in my group if you are motivated by using your QM/MM and MD skills to study the fascinating histone post-translational modifications! 🥳
Deadline is the 1st of March #job #compchem #histones #DNA
Apply on the CNRS website : lnkd.in/eNxR-TPH

The #EMGS is now seeking a new Editor-in-Chief for
@EMMjournal! In this pivotal role, the new EIC will influence EMM’s direction and uphold its high scientific standards as the official journal of the EMGS!

Learn More: emgs-us.org/news/687716/...

Postdoctoral Research Associate in Biochemistry and Molecular Genetics – Weaver Lab in Charlottesville, Virginia, United States of America | Research at University of Virginia Apply for Postdoctoral Research Associate in Biochemistry and Molecular Genetics – Weaver Lab job with University of Virginia in Charlottesville, Virginia, United States of America. Research at Univer...

My lab is looking to hire a Postdoc to study mechanisms of DNA repair in chromatin using biochemical, biophysical, and structural biology approaches! Feel free to share with anyone you think may be interested.

jobs.virginia.edu/us/en/job/R0...

Pathway to independence: a new initiative supporting postdocs on the job market

Our Pathway to Independence programme aims to support postdocs going on the job market in 2025 with training, mentorship, profile-raising and networking opportunities.

Apply by 31 Jan 2025: https://buff.ly/3VJGgDC

#DevBio #StemCells🧪

We’re quickly becoming the very best of friends. 😂

The Weaver Lab is officially open today…Let’s see if we can fill up this empty lab space!

Is chromatin ordered or disordered? It all depends on the linker DNA length.

Check our latest work with Mike Rosen and Sy Redding. We explore how changes in linker DNA length (as small as 1 bp) fine-tune chromatin structure, between order and disorder, and the properties of chromatin droplets

Also nice to see recent work with similar mechanistic insight! www.nature.com/articles/s41...

Great collaboration between the @freudlab.bsky.social, @k4v1.bsky.social , David Cortez, and Steve Roberts labs. The penultimate piece of work from my postdoc.

Our preprint from earlier this year on the oxidation-induced mutational landscape in human cells was just published! www.nature.com/articles/s41...

I’m still amazed how much chromatin structure impacts oxidation-induced mutagenesis. Our cryo-EM contribution in this work provides a glimpse into why!

Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNA Quiescence in Saccharomyces cerevisiae is a reversible G0 crucial for long-term survival under nutrient-deprived conditions. During quiescence, the genome is hypoacetylated and chromatin undergoes sig...

a short and sweet paper in which we use 3D STED and Micro-C analysis to show dramatic changes in large 3D chromatin structures in quiescence. www.biorxiv.org/content/10.1...

Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation and function remain incompletely understood due to difficul...

www.biorxiv.org/content/10.1... incredible study from Huabin Zhou and the Villa & Rosen labs -- a blueprint for mapping human chromatin with cryo-ET. The future of chromatin structural analysis in situ has arrived.

Today was the last day in lab for @tmweaver.bsky.social. We enjoyed lunch and gave him a 3D print of his first cryo-EM structure.

Tyler's era was marked by incredible science, friendship, fun, collabs, and cats! He will do amazing at UVA running his own lab. We will miss you buddy #gogetem 🥹

Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells - Nature Communications Non-homologous end joining repairs most double stranded DNA breaks in human cells. Here, the authors apply a single-molecule imaging approach visualizing the recruitment of DNA repair proteins to DNA ...

Super excited to share our latest paper led by graduate student Mariia Mikhova! She used single molecule live cell imaging to dissect the kinetics of DNA break repair by non homologous end joining. We estimate the NHEJ pathway can repair up to 1000 breaks per minute!

www.nature.com/articles/s41...

Thymine DNA glycosylase combines sliding, hopping, and nucleosome interactions to efficiently search for 5-formylcytosine - Nature Communications Thymine DNA glycosylase is an essential DNA repair enzyme involved in oxidative demethylation of 5- methylC. In this study the authors have tracked specific and non-specific DNA binding of TDG at the ...

Happy to celebrate Brittiani Schnable's PhD work on thymine DNA glycosylase! www.nature.com/articles/s41...

Phosphorylation regulates the chromatin remodeler SMARCAD1 in nucleosome binding, ATP hydrolysis, and histone exchange Maintaining the dynamic structure of chromatin is critical for regulating the cellular processes that require access to the DNA template, such as DNA …

Our paper on the strange ATP-dependent chromatin remodeler Smarcad1 paper is out and freely accessible. Differential regulation of its many activities by phosphorylation. Congrats to Briana and @nhoitsma.bsky.social 🧪www.sciencedirect.com/science/article/pii/S002...

My lab at UVA-SOM will be hiring at all levels in January, so please get in touch if you’re interested!

Time to introduce myself! Incoming Assistant Professor at UVA-SOM. Interested in chromatin structure and DNA repair.

Here is some of my final work in the @freudlab.bsky.social, where we determine how 1-nt gaps in the nucleosome are filled during DNA repair.

www.biorxiv.org/content/10.1...