Hello bluesky

Engineering plant tandem kinase immune receptors expands effector recognition profiles Plant intracellular immune receptors are widely deployed in breeding to protect crops from disease. In addition to nucleotide-binding leucine-rich repeat receptors (NLRs), tandem kinase proteins (TKPs...

Tandem Kinases containing HMA domains can be engineered to sense additional effectors. Parallels with NLR HMA engineering and highlights the versatility of this interesting integrated domain. www.biorxiv.org/content/10.6...

Evolution of HMA-integrated tandem kinases accompanied by expansion of target pathogens Tandem kinase proteins (TKPs) are an emerging family of plant intracellular immune receptors that offer potential for developing novel disease resistance. We cloned Rmo2 and Rwt7, genes for resistance...

Another exciting preprint! Two additional HMA-TKP sensors from wheat and barley, swapping HMAs can confer recognition specificity. Evo analyses supporting HMA-TKP has differentiated to recognize different effectors through HMA diversification. other links below. www.biorxiv.org/content/10.6...

Super-happy sharing my Lab's new work on engineering plant HMA-containing tandem kinase immune receptors. Congrats to all authors (@danielsyu.bsky.social and @njw33.bsky.social on here). Work only possible in collaboration with Soichiro Asuke. See linked paper to follow!
bsky.app/profile/bior...

(5/5) Incorporating the engineered HMAs domains into TKP receptors, we demonstrate the engineered receptors have expanded recognition profiles in cereal protoplast assays.

(4/5) The effector binding interfaces on the HMA domains of Rmo2 and Rwt7 are spatially distinct. We engineered both TKP-HMAs to gain binding to non-cognate effectors in vitro and in plant cells.

(3/5) Based on these structures, we mutated the effectors disrupting TKP-HMA binding. This prevents TKP recognition responses in infection assays confirming the role of the TKP-HMA as an integrated effector bait domain.

(2/5) We experimentally solved the co-structures of TKP-HMA domains from Rmo2 and Rwt7 with cognate effectors, PBY2 and PWT7, respectively and show they bind tightly and specifically to the effectors that they recognise.

Evolution of HMA-integrated tandem kinases accompanied by expansion of target pathogens Tandem kinase proteins (TKPs) are an emerging family of plant intracellular immune receptors that offer potential for developing novel disease resistance. We cloned Rmo2 and Rwt7, genes for resistance...

(1/5) Rmo2 and Rwt7 are TKPs that mediate immunity to blast disease in barley and wheat, respectively. Both receptors contain an integrated HMA domain. Check out the preprint from our collaborators:
www.biorxiv.org/content/10.6...

Engineering plant tandem kinase immune receptors expands effector recognition profiles Plant intracellular immune receptors are widely deployed in breeding to protect crops from disease. In addition to nucleotide-binding leucine-rich repeat receptors (NLRs), tandem kinase proteins (TKPs...

Excited to share our #plantimmunity preprint on engineering cereal TKPs
www.biorxiv.org/content/10.6...
A short thread below 👇

Architects of plant immunity: Structure-informed strategies for engineering plant nucleotide-binding leucine-rich repeat receptors Diseases caused by plant pathogens are a major factor decreasing crop yields that lead to food insecurity. To protect against pathogen threats, plants…

Excited to share our review on structure-based engineering of NLRs in plant immunity. We also have some cool plant immunity engineering stories coming out this month so watch this space! #plantimmunity #nlr

www.sciencedirect.com/science/arti...

Registrations are rolling in for the 2025 Stromlo Plant Pathology meeting in Canberra (Nov 27–28)! Secure your spot! Speakers: @yasukadota.bsky.social, Cecille Segonzac, @thamlab.bsky.social, @yichangsung.bsky.social, Tek Tay & @danielsyu.bsky.social. Register at tinyurl.com/3e3tx9j2
#strompath25

Super excited to be giving a talk at #2025ISMPMI today in the Synthetic biology concurrent session from 15:15 - 15:35! Come along if you’re interested in engineering Tandem Kinase Proteins

Group Leader in Discovery Plant Sciences | John Innes Centre The John Innes Centre (JIC) seeks a visionary Group Leader to lead research on Discovery Plant Science.

GROUP LEADER VACANCY

JIC is recruiting a Group Leader in Discovery Plant Sciences.

If you think your research would synergise and thrive at JIC, please apply.

www.jic.ac.uk/vacancies/gr...

A plant dual-kinase protein traps a pathogen effector to trigger immunity - Nature Genetics Tandem kinase proteins (TKPs) are key regulators of plant immunity. We found that RWT4, a wheat TKP, confers resistance to the fungal pathogen Magnaporthe oryzae by directly recognizing the effector A...

Our Research Briefing on the tandem kinase proteins paper is online now!
Curious about the story behind our TKP paper?
📄 Research Briefing: www.nature.com/articles/s41...
📄 Research article: www.nature.com/articles/s41...

Plant pathogenic fungi hijack phosphate signaling with conserved enzymatic effectors Inorganic phosphate (Pi) is essential for life, and plant cells monitor Pi availability by sensing inositol pyrophosphate (PP-InsP) levels. In this work, we describe the hijacking of plant phosphate s...

Its out....

Plant pathogenic fungi hijack phosphate signaling with conserved enzymatic effectors | Science www.science.org/doi/10.1126/...

A wonderful collaboration with the Schaffrath @rwth.bsky.social and Oliveira-Garcia groups! Huge credit to Carl Mcombe and Alex Wegner.

HI old friends! Finally took the leap here to say I have several open positions going in my lab at the moment (and more to come).
1) PhD studentship in collaboration with the Royal Horticultural Society (genomics)
2) Biochemistry Postdoc to understand how Starships move

Check out the links below!

If you're from the UK and are looking for a PhD, don't miss out on this fantastic opportunity! Amazing lab and amazing project!

Yes, that was made in blender too!

Excited for more #sciart! Here is a #blender render of a small #fusarium effector I solved during my PhD

In case you missed it, here is the TSL Autumn Symposium 2024 in full 🍿

📣 PhD opportunity at @thesainsburylab.bsky.social Norwich, UK.
"Investigating host penetration by the rice blast fungus" with Dr Frank Menke and Nick Talbot
@FrankMenke.bsky.social @talbotlabtsl.bsky.social

Apply before 25 Nov 2024! ⬇️
biodtp.norwichresearchpark.ac.uk/projects/inv...

So many keen peeps interested in structural biology.. we have a new unit cell. 🧶🧬
go.bsky.app/HByHiW6

Could you add me please 🤩

I created this Starter Pack for PhDs and Postdocs in Structural Biology to help connect with colleagues who share similar interests and concerns in the field.

Feel free to recommend people or yourself! I'll be adding people 💪🏼😁

bsky.app/starter-pack...

Add me please 🤩

Hello new Plant Scientists,

To get you started, the two #PlantScience Research #StarterPacks will connect you to other Scientists & relevant feeds:
1) go.bsky.app/8Zfmwf7
2) go.bsky.app/3NcqRCE

You can find more relevant Plant Science feeds here:
bsky.app/profile/soms...

Further... 1/2

First post on bluesky! Sharing some photogenic protein crystals I got a few months back 💎. Shame they didn’t diffract as well as they look

Hi everyone,
I created a starter pack for all fungal biologists.
Tell me who I should add or if you want to be part of it!
#fungi

go.bsky.app/12PCCjU