Now available - The Nanosoft 8-Pocket Autogrid Box - A #cryoem grid box with 8 pockets designed, shaped & sized for autogrids - Pockets are offset to make autogrid removal easier - Available in 6 colors & with a random, unique 5-digit serial number -> zurl.co/89dEH
Calling all xray structure folks that have worked with denovo proteins. Do you suffer from high rfactors? Myself, someone else I know and now multiple structures all seem to suffer from this. Assuming we all tried our best in model building, what could be some causes?
New preprint 🚀 ❄️
We developed Autogrid-compatible HPF planchettes that enable direct freezing of clipped EM grids and streamline integration with cryo-FIB workflows
✔️ improved grid stability
✔️ reduced handling
✔️ robust vitrification (🦠 🧫 🧠)
A step toward routine HPF for complex samples
#teamtomo
Grappling with poorly-resolved ligand binding sites in #cryoEM maps?
Check out our new case study on a peptide ligand-bound GPCR showing how practical tips and features in #CryoSPARC v4.7 improve locally refined map quality and reveal clearer ligand density!
guide.cryosparc.com/processing-d...
Our work is the first study to provide structures of several tRNAs before and after the introduction of modifications at specific sites. In detail, we analyzed 4 human tRNA species to decipher structural and stability effects induced by the installation of Ψ at single or multiple sites.
We used single particle cryo-EM to directly visualize several human tRNAs and analyze the effects of individual tRNA modifications. To our knowledge, the work presents interpretable maps of the smallest asymmetric particles analyzed by single-particle cryo-EM so far.
We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵
Reanalysis of cryo-EM data from EMPIAR entry EMPIAR-10,930 deposited by Reshetnyak and colleagues, Nature (2021). (A) Ten most populated 2D class averages after performing 2D classification on 11.8 million selected particles. Classes corresponding to 2:1 ALK–ALKAL2 particles are highlighted in blue, while classes corresponding to 2:2 ALK–ALKAL2 particles are highlighted in orange. (B) Ab initio model generation in cryoSPARC results in classes corresponding to both a 2:1 ALK–ALKAL2 and 2:2 ALK–ALKAL2 assembly. Hetero-refinement of the 2:1 and 2:2 ALK–ALKAL2 classes results in the two models displaying 2:1 and 2:2 ALK–ALKAL2 assemblies, respectively. The ALK–ALKAL2 complexes with 2:1 (PDB 7nwz) and 2:2 (PDB 7n00) stoichiometries are shown fitted inside the maps, with ALK colored pink/gray and ALKAL2 colored blue/purple. (C) 3D refinement; a front view is shown of a map sharpened in RELION using a B-factor of −100 Å2, with ALK colored pink/gray and ALKAL2 colored blue. Front, side, and top views are shown of a transparent map, sharpened using deepEMhancer, with fitted structural model.
Reports of diverse stoichiometries have complicated our understanding of the complex between ALK kinase and its #cytokine ligand ALKAL2. @janfelix.bsky.social @savvideslab.bsky.social &co reanalyze #cryoEM data to clarify the situation @plosbiology.org 🧪 plos.io/4lvRfvu
Does anyone have any idea what this wavey guy is? 🌊🌊🌊🌊#TeamTomo #CryoET
In-situ tomogram of milled RPE-1 cells.
Join us at Amgen Structural Biology in SoCal! Work on #xray and #cryoEM.
careers.amgen.com/en/job/thous...
Fast event-based electron counting for small-molecule structure determination by MicroED pubmed.ncbi.nlm.nih.gov/39982366/ #cryoEM
Niko Vlahakis et al.: Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination #CryoEM #MicroED #Nanocrystals @ucla...#IUCr https://journals.iucr.org/paper?S2052252524012132
Interested in #CryoEM structure refinement?
Then this #webinar is for you: "Cryo-EM structure refinement with density-guided simulations in #GROMACS"
🗓️ 4 March 2025, 15:00 CET
Registration & further info ➡️ bit.ly/41b6L7W
Mystery Protein Challenge Calling all structural biology enthusiasts and #cryoEM aficionados!
Can you identify the protein shrouded in this 2D class average?
Hint:
This protein plays a vital role in the respiratory chain.
Are you doing cell #biology? Then, let me guess, you likely stained #actin!
Our @jcb.org article reveals the structure of the popular actin probe F-tractin in complex with F-actin and introduces an optimized version for better imaging! A thread! 👀👇
rupress.org/jcb/article/...
Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination pubmed.ncbi.nlm.nih.gov/39927752/ #cryoEM
(BioRxiv All) CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes from Azotobacter vinelandii: Single particle cryoelectron microscopy (cryoEM) and cryoelectron tomography (cryoET) are powerful methods for unveiling… http://dlvr.it/THnVMD #BioRxiv #MassSpecRSS
Complex Water Networks Visualized through 2.2-2.3 A Cryogenic Electron Microscopy of RNA pubmed.ncbi.nlm.nih.gov/39896454/ #cryoEM
- identifies crucial contacts; very useful for selecting mutagenesis targets
- fixes model geometry. In cryo-EM, stereochemistry is usually the best priors you have!
- helps predicting models of weak complexes that AlphaFold3 cannot
I’m happy to share gapTrick, an AlphaFold2-based tool for characterising protein-protein complexes
www.biorxiv.org/content/10.1...
Are PDB models biased in terms of how well they explain the underlying experimental data and are modeled?
www.biorxiv.org/content/10.1...
Our findings emphasize how we use PDB models. It is critical to understand that PDB models are just that, models. They do not fully explain the underlying experimental data, have nuances in how they are representing the data, and as we show, can vary in how well the underlying data is explained.
The difference between a model and the underlying crystallographic information is shown for the disordered MOF published in this paper: https://pubs.acs.org/doi/10.1021/jacs.2c13731. The model shows a ball&stick atomic structure, whereas the information (electron density calculated from structure factors) is displayed as density map with coloured contours.
Casual reminder that what many consider 'information' from #crystallography is actually a model. That is why structure factors must *always* be published, so that reviewers and anyone can see what lies behind the model. Without them, crystal structures are nothing more than 'pictures' in 3D.
Following a suggestion from @benecal.bsky.social I will refer to these threads as "skeetorials" and will use this gif logo.
Today's skeetorial is about the NIH Director's New Innovator Award with three different heroes (all in government).
1/n
Who are some amazing cryoEM/cryoET, mass spec, and light microscopy scientists coming to San Diego for ASCB? I’m making a list for a potential pre-ASCB workshop at UCSD December 13th. Spread the word and let me know if you know a good candidate to speak at such an event!
Here is a CryoEM starter pack I try to assemble
go.bsky.app/JmQksC
