Thank you very much for sharing this cool animation!

Organoids stained with Spirochrome probes for actin and DNA! 😍

See how PKMito dyes promote mitochondrial research😀
@spirochrome.com

🙏 @christlet.bsky.social for the cool "Animated Zooming" & "Animated panning" ImageJ plugins used here 😎

Beware the SPY488-FastAct rollercoaster!

We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct.

Find out more about SPY488-FastAct: spirochrome.com/product/spy4...

📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!

There is now a very detailed #JOVE video that explains it all step by step.

Video link: dx.doi.org/10.3791/6940...

Peroxi_SPY link: spirochrome.com/products/?_c...

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📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!

There is now a very detailed #JOVE video that explains it all step by step.

Video link: dx.doi.org/10.3791/6940...

Peroxi_SPY link: spirochrome.com/products/?_c...

🧪

🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.

These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).

Movie from preprint www.biorxiv.org/content/10.6...
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HAK-actin™ - Actin Expansion Microscopy Probe - Spirochrome HAK-actin™ is a pan-species Expansion Microscopy (ExM) compatible probe for actin visualization using U-ExM based protocols

HAK-actin product link: spirochrome.com/product/hak-...

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.

These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).

Movie from preprint www.biorxiv.org/content/10.6...
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🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁

Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)

Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

5/5 The Takeaway: Flipper-TR didn't just report CMT; it identified CMT as a master switch linking physics to genetics.

From development to disease, this probe proved that how "tight" a cell is matters just as much as its DNA.

Read the full study: www.biorxiv.org/content/10.6...

4/5 The "Stuck" State: Flipper-TR also exposed a fibrosis mechanism.

In healthy lungs, tension drops to allow repair. But in fibrotic lungs (IPF), Flipper-TR showed tension stays high.

This high tension state traps cells in a dysfunctional "transitional state," preventing healing.

3/5 Mapping the Invisible: Using Flipper-TR + FLIM imaging, researchers visualized tension in live lung tissue.

They discovered a drop in membrane tension before cells differentiate into AT1 or AT2 alveolar cells.

Even cooler? It mapped "emergent zones" of low tension where differentiation starts!

2/5 The Challenge: Scientists knew various signals guided lung differentiation, but how do cells "know" when to specialize?

Here comes Flipper-TR: A mechanosensitive dye that changes its fluorescence lifetime based on CMT. High tension = Long lifetime; Low tension = Short lifetime

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁

Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)

Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰

Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs:

www.biorxiv.org/content/10.6...

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday

Easy to use: just dilute the probe in cell culture medium and image 30 min later!

🔗 spirochrome.com/product/spy6...

🙏 Big thanks to @lazaresaladin.bsky.social for the images.
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Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday

Easy to use: just dilute the probe in cell culture medium and image 30 min later!

🔗 spirochrome.com/product/spy6...

🙏 Big thanks to @lazaresaladin.bsky.social for the images.
🧪

We love how amazing our SPY650-DNA probe looks on your microscopy images.
Congratulations to everyone involved!

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list!

Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript.

Congratulations to the team!

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X.

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ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥
Thank you @j-today.bsky.social for posting this gem!

Amazing movie! We love to see our probes SPY555-FastAct_X and Sir-tubulin perform so well in your hands! 🤩

Thank you for sharing.

Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells.

Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!

Thank you Kim for your amazing work! We love your designs and working with you on the HAK-actin™ project was fast, efficient and a real pleasure!