Primer design for 3' end of polyadenylated transcripts for targeted RNA seq, in bulk, single-cell, or spatial mode. New from OncoRNALab at @crighent.bsky.social

Blood (literally and figuratively), sweat, and tears... rejection after 3 rounds of revisions by another journal, but now out. We are proud of this new type of biomarker identification concept - applied here on cell-free RNA from cancer patients @amorlion.bsky.social
www.nature.com/articles/s43...

The spatial team at OncoRNALab @ds-ugent.bsky.social posted a benchmarking preprint on DNA barcode decoding strategies www.researchsquare.com/article/rs-8... @brechtsoulliaert.bsky.social

Marvin Heyer on 260K 3.7 pL partitions- new dPCR format at #EUDIP2025

Phage quantification by Stefan Vermeulen at #EUDIP2025

“I have 9 qPCR instruments” Stephen Bustin at #dPCR #EUDIP2025 meeting in Ghent!

Still possible to follow online. #EUDIP2025 All about digital PCR #dPCR digpcr.ugent.be/EUDIP2025/ great talks by Allen Chan, Noemi Castellucci, Wim Trypsteen.

#EUDIP2025 kicks off in Ghent! All about digital PCR #dPCR digpcr.ugent.be/EUDIP2025/

The model of harmonizing general and domain-specific guidelines provides a scalable blueprint for improving reproducibility across complex biomarker development workflows in molecular diagnostics. The case for EV and qPCR www.oaepublish.com/articles/evc...

We’re so proud! RNA rocks, and you too!

Is rubber then #antifragile?

85 m?

Originally at least 3, we (and #MIQE) now recommend at least 2 stably expressed reference genes, and more if needed. geNorm can help determine how many are needed.

9 (vanop klein schermpje)

Are you also a desperate cell-free RNA researcher? It’s not always a grim story. Philippe Decruyenaere is defending his excellent PhD thesis on extracellular RNA in non-Hodgkin lymphoma. @crighent.bsky.social

... perfect uit welk beloftevol onderzoek ze aan het doen is.
www.eoswetenschap.eu/gezondheid/e...

Of zoals Hetty Helsmoortel (een voormalige onderzoeker uit ons lab waar we trots op blijven) het verwoordt: "Het moet niet precies zijn, maar wel juist."

Veel leesplezier!

"Eindelijk beeld bij het verhaal van onze genen"

Dit artikeltje wou ik toch even delen. Wetenschappelijk onderzoek helder uitleggen is niet makkelijk, en toch is het belangrijk. Hier legt Hanne, een doctoraal onderzoeker in ons OncoRNALab (@crighent.bsky.social @ds-ugent.bsky.social) ...

I'm incredibly proud of this unique study, not only because of its scope, size, and outcome, but also because of the way so many have contributed over a large period and for the way we circumvented classic linear authorships.

#CRediT #blood #plasma #RNA

Enjoy the 'Behind the paper' story written by two talented postdoctoral researchers in our OncoRNALab, Anneleen Decock and @amorlion.bsky.social . The paper itself is cited at the top of the blog.

The road to discovery - a collaborative effort towards new insights in blood plasma RNA profiling After more than seven years of research, our extracellular RNA quality control (exRNAQC) study has culminated in a scientific publication. This milestone is not just about sharing new findings – it re...

7 years in the making... 1.6 liters of blood and 11 billion sequencing reads to compare 456 extracellular transcriptomes.

Our best practices for blood plasma extracellular RNA profiling have been published as a peer-reviewed article.

communities.springernature.com/posts/the-ro...

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines AbstractBackground. In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for

After 16 years, an update of the qPCR #MIQE guidelines is out on how to design, execute, analyze, and report on #qPCR experiments doi.org/10.1093/clin.... The original paper from 2009 accumulated 17,000 citations (scholar.google.be/citations?vi...). @genequan.bsky.social @jimhuggett.bsky.social

Ik ben dankbaar voor het vertrouwen dat aan ZAP is gegeven en voor de start van basisfinanciering, waar wel (beloofde) correcties nodig zijn, zoals een domein multiplicator.

👉 Finally the update of the MIQE Guidelines is out in Clinical Chemistry 👍
𝗠𝗜𝗤𝗘 𝟮.𝟬: 𝗥𝗲𝘃𝗶𝘀𝗶𝗼𝗻 𝗼𝗳 𝘁𝗵𝗲 𝗠𝗶𝗻𝗶𝗺𝘂𝗺 𝗜𝗻𝗳𝗼𝗿𝗺𝗮𝘁𝗶𝗼𝗻 𝗳𝗼𝗿 𝗣𝘂𝗯𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗤𝘂𝗮𝗻𝘁𝗶𝘁𝗮𝘁𝗶𝘃𝗲 𝗥𝗲𝗮𝗹-𝗧𝗶𝗺𝗲 𝗣𝗖𝗥 𝗘𝘅𝗽𝗲𝗿𝗶𝗺𝗲𝗻𝘁𝘀 𝗚𝘂𝗶𝗱𝗲𝗹𝗶𝗻𝗲𝘀
lnkd.in/dhWhtZqC

Clonal Hematopoiesis as a Driver of Solid Tumors | NEJM Clonal hematopoiesis is the expansion of a genetically related population of hematopoietic stem and progenitor cells that disproportionately contribute to blood-cell production.1 Clonal hematopoies...

New evidence @nejm.org today that age-related clonal hematopoiesis (CHIP) has a driver role in evolution of solid cancers
www.nejm.org/doi/full/10....
www.nejm.org/doi/full/10....

Ik heb zopas de resulaten doorgestuurd van de waterkwaliteit in de Tichelbeek t.h.v. de Brandstraat in Zulte, als deel van het grootste burgeronderzoek ooit. Ben je ook benieuwd naar de resultaten? Neem dan een kijkje op www.watermonsters.be. Vanaf 7 juni zijn alle resultaten gekend. #watermonsters

Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids - Nature Protocols The authors present a protocol for the self-assembly of single-cell suspensions into spheroids by the liquid overlay method, followed by a modular framework of orthogonal assays to phenotype spheroids...

Proud OncoRNALab contribution to #CRIG LECR lab’s latest protocol paper on spheroids www.nature.com/articles/s41...

Why we study shrimp on treadmills: The case for curiosity-driven research “If we cut or limit funding for curiosity-driven research, we risk shutting down the pipeline of future innovation,” writes Carole LaBonne of Northwestern.

The case for curiosity-driven research www.statnews.com/2025/04/03/b... h/t former OncoRNALab PhD fellow @verwilt.bsky.social

Wat onze lichaamssappen ons vertellen Rond 400 voor Christus ontwikkelde Hippocrates, de grondlegger van de moderne geneeskunde, zijn leer der humores. Hij stelde dat de gezondheid van de mens af...

Ever wondered what RNA in your body fluids can reveal about your health? 🤔 Eva Hulstaert took on the challenge, collecting and analyzing various biofluids to uncover hidden insights. 👩‍🔬 Anneleen Decock & I share the story of this fascinating quest in EOS Wetenschap (in Dutch): tinyurl.com/4ev7vk83

My colleague Jan Gettemans at Ghent University will reach out to 1st author. He received email yesterday.

Did you know that our body contains dozens of different biofluids (like blood, sweat, urine, saliva, ...) and that these give insights into our health? OncoRNALab members @amorlion.bsky.social and Anneleen Decock wrote a fascinating story (in Dutch) in the science magazine Eos - lnkd.in/ern_2kDW