#MIQE

Originally at least 3, we (and #MIQE) now recommend at least 2 stably expressed reference genes, and more if needed. geNorm can help determine how many are needed.

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines AbstractBackground. In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for

After 16 years, an update of the qPCR #MIQE guidelines is out on how to design, execute, analyze, and report on #qPCR experiments doi.org/10.1093/clin.... The original paper from 2009 accumulated 17,000 citations (scholar.google.be/citations?vi...). @genequan.bsky.social @jimhuggett.bsky.social