🚨 New paper alert 🧬
Our work from the @michellepeckham.bsky.social lab is now out in #ScienceAdvances.

We present the #cryoEM structure of shutdown human non-muscle myosin 2A, showing how ionic interactions stabilise auto-inhibition and how mutations disrupt this state.

👉 tinyurl.com/4de8teyh

Very grateful to collaborators both studies included Woolfson lab, Berger-Schaffizels lab and @jessicacross.bsky.social labs at Bristol Biochem/Chem alongside JJ Phillips lab at Exeter

We think that this is probably the tip-of the iceberg in understanding how kinase signalling can direct control intracellular transport by kinesin-1

Second, a new preprint examining how binding of cargo adaptors is integrated with direct association of the motor complex with membranes is available on bioRxiv. We identify the kinase NEK10 as a Kinesin-1/KLC2-selective negative regulator of lysosome transport www.biorxiv.org/content/10.6...

We thought the new open and transparent process @elife.bsky.social was excellent and appreciated valuable feedback from the reviewers.

Kinesin-1 conformational dynamics are controlled by a cargo-sensitive TPR switch Cargo-adaptor binding to the KLC-TPR domains binding triggers structural rearrangements that relieve kinesin-1 autoinhibition, enabling MAP7 recruitment, through an allosteric mechanism that connects ...

Two manuscripts from the lab to catch up upon exploring aspects kinesin-1 regulation. First, our examining the role of cargo-adaptor binding in controlling kinesin-1 conformational dynamics and association with MAP7 is now a VOR @elife.bsky.social elifesciences.org/articles/109...

And a postdoc position available working between our lab and the Woolfson lab designing proteins and peptides to augment intracellular transport pathways t.co/0WWgylZMbS

Molecular mechanism for cargo-mediated initiation of kinesin-1 activation Kinesin-1 is a dynamic heterotetrameric assembly of two heavy and two light chains (KHC and KLC) that mediates microtubule-based intracellular transport of many different cargoes. The complex adopts a...

New preprint on kinesin inhibition and activation - collaborative effort across Bristol Biochem and Chemistry with Woolfson and Schaffizel labs and JJ Phillips in Exeter 1/2 www.biorxiv.org/content/10.1...

We have a new 4-year postdoctoral position available working between our lab at the Woolfson lab at Schools of Biochemistry and Chemistry at Bristol. Applying protein design approaches to control intracellular transport. More info on link and flyer. Get in touch! www.bristol.ac.uk/jobs/find/de...

Funded PhD- Coordination Between Actin and Microtubule Motors During Endocytosis at University of Bristol on FindAPhD.com PhD Project - Funded PhD- Coordination Between Actin and Microtubule Motors During Endocytosis at University of Bristol, listed on FindAPhD.com

The position is either funded by a UoB scholarship or SWBio DTP - to be considered for both pools of funding apply separately for each of these. Secondary sup. is the brilliant @markdodding.bsky.social. 📧 E-mail Ferdos.abidali@bristol.ac.uk for informal enquiries & 📝apply here tinyurl.com/yvzeb2kw.

Please share this PhD opportunity in my new lab in Bristol, using protein design/ engineering to understand and build molecular motors to control intracellular transport. Working with @markdodding.bsky.social and Prof Dek Woolfson.
www.findaphd.com/phds/project...
www.swbio.ac.uk/biomolecular...

We have three PhD projects with motor-cytoskeleton theme though collaborations with three new(ish) PIs at Bristol Biochemistry supported by SWBio_DTP with Berni Carroll (lysosome transport), @abidalilab.bsky.social (endocytosis) and Jessica Cross (protein design)
see swbio.ac.uk/biomolecular...