Look delicious!

Special shoutout to Wei Qin from @varshneylab.bsky.social lab. He has done the hard work to make precise editing in zebrafish a snap! Also shout out to all the other speakers in the series!

Congratulations!

Cleaning freezers before I move to a new place. Found these plates from @burgesslab.bsky.social, these plates made my career, hard to throw them now.

Conspiracy against Pis!

Is Ensembl dead? Never worked for me for the last few months.

A powerful new technology developed at OMRF could dramatically speed up the process of diagnosing rare genetic diseases.

Read more: omrf.org/2026/02/03/o...

High-efficiency TadA cytosine base editors for precise modelling of human disease variants Nature Biomedical Engineering, Published online: 28 January 2026; doi:10.1038/s41551-025-01607-1A suite of TadA-derived cytosine base editors was optimized to improve efficiency, reduce sequence bias, expand PAM compatibility and minimize artefacts, enabling precise genetic interrogation of variants of uncertain significance (VUS) in zebrafish.

High-efficiency TadA cytosine base editors for precise modelling of human disease variants

Thanks Shawn! You trained me well.

TCBE-Umax delivers:
✓ 2x higher efficiency
✓ Works across diverse sequence contexts
✓ Minimal indels
✓ Expanded PAM options for hard-to-target sites
Grateful to ORIP/NIH and the Presbyterian Health Foundation for supporting this work.

High-efficiency TadA cytosine base editors for precise modelling of human disease variants - Nature Biomedical Engineering A suite of TadA-derived cytosine base editors was optimized to improve efficiency, reduce sequence bias, expand PAM compatibility and minimize artefacts, enabling precise genetic interrogation of vari...

Our paper on TCBE-Umax is out in @natbiomedeng.nature.com!
We developed these high-efficiency cytosine base editors for zebrafish, enabling rapid functional testing of disease variants. This work is led by a talented scientist, Wei Qin.
www.nature.com/articles/s41...

Folks, it is finally out! Our paper on T2T assemblies of the zebrafish genome is on BioRxiv:
www.biorxiv.org/content/10.1...

Structures of DNA and morpholino oligonucleotides. R and R' denote continuation of the oligomer chain in the 5' or 3' direction, respectively. Credit to Corey & Abrams (2001) Genome Biology.

Published October 25 years ago, “Effective targeted gene ‘knockdown’ in zebrafish” by Nasevicius & Ekker detail use of antisense morpholino-modified oligonucelotides or #morpholinos that inhibit translation. This landmark spurred a revolution in targeted gene knockdown in vivo. #ZebrafishFunFacts 🧪

While I'm sad our cover submission wasn't selected for our recent human duplication paper, check out the super cool illustration created with @lazaroillustration.bsky.social ! Certainly, you will be seeing Dennis lab members running around with "fishman" t-shirts at future meetings, inspired by Ohno

Does anyone have #zebrafish LC3 reporter lines and would be willing to share them with us? #askzebrafish

#zebrafish genome update, our T2T assembly of the inbred strain of AB (M-AB) generated by my buddy Nori Sakai has now been released at NCBI and will be a second reference genome for zebrafish (GRCz12ab):

Best wishes!

I do it at least 2-3 times a day!

Even if you do it from a phone, it will not be cleared elsewhere!

Congratulations!

CRISPR-based functional genomics tools in vertebrate models - Experimental & Molecular Medicine Recent advances in DNA sequencing have allowed scientists to gather vast amounts of genetic data, but understanding what those sequences actually do requires additional tools that allow scientists to ...

CRISPR technologies have transformed genetic research since their adaptation for genome editing in 2012. @burgesslab.bsky.social and I summarize key functional genomics tools being used in vertebrate models. www.nature.com/articles/s12...

Congratulations, Megan! As always inspiring work.

By engineering TadA-based cytosine base editors with enhanced PAM flexibility and reduced off-targets, we overcome key limitations of existing CBEs, including poor editing at CC and GC motifs.

Rationally Designed TadA‐Derived Cytosine Editors Enable Context‐Independent Zebrafish Genome Editing A next-generation cytosine base editor (zTadCBE) and other variants are developed by combining the high-efficiency (TadCBEa) and low-indel (TadCBEMax) architectures, followed by the introduction of z....

Our paper on TadA-based cytosine base editors is now online in Advanced Science. We introduce zTadCBEs—a next-generation CRISPR base editor optimized for zebrafish.
advanced.onlinelibrary.wiley.com/doi/10.1002/...

Congratulations!

🚨🚨🚨 Tol2kit announcement! We have rebuilt the wiki that was corrupted a few months ago! We’re in the process of also linking it to the original address. Please visit us here:
tol2kitkwan.genetics.utah.edu
Thank you for your patience!!
#zebrafish #transgenesis #plasmids #sharing

Dear #izfc2025 participants, if anyone of you are interested in how transcription control defines mRNA fate and translation in zebrafish development, there are/will be several PhD and postdoctoral positions (funded by the Wellcome Trust) for this research. Please find me if interested.

The winners of the 2025 George Streisinger Award are the Course Directors of the Zebrafish Development and Genetics course at MBL, for their tremendous contributions to our amazing community for >25 years! #izfc2025

The new impact factors are out: Development – 3.6, Developmental Cell – 8.7. Why the steady decline in two of Dev Bio's flagship journals?