Structures of DNA and morpholino oligonucleotides. R and R' denote continuation of the oligomer chain in the 5' or 3' direction, respectively. Credit to Corey & Abrams (2001) Genome Biology.

Published October 25 years ago, “Effective targeted gene ‘knockdown’ in zebrafish” by Nasevicius & Ekker detail use of antisense morpholino-modified oligonucelotides or #morpholinos that inhibit translation. This landmark spurred a revolution in targeted gene knockdown in vivo. #ZebrafishFunFacts 🧪

Structures of DNA and morpholino oligonucleotides. R and R' denote continuation of the oligomer chain in the 5' or 3' direction, respectively. Credit to Corey & Abrams (2001) Genome Biology.

Published 25 years ago, “Effective targeted gene ‘knockdown’ in zebrafish” by Nasevicius & Ekker is the 2nd most-cited #zebrafish paper (www.nature.com/articles/ng1...). They describe use of antisense morpholino-modified oligonucelotides or #morpholinos that inhibit translation. #ZebrafishFunFacts 🧪