New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of #FluorescentProteins #ProteinEngineering #MachineLearning #SyntheticBiology 1/n

"Large-scale synthetic gene libraries for decoding variant effects in antibiotic resistance" YouTube video by Variant Effects

🎥 My Variant Effects Seminar Series (VESS) talk is now on YouTube! I discuss how DropSynth-based gene synthesis reveals how bacterial DHFR variants evolve antibiotic resistance. Thanks to @varianteffect.bsky.social for the invite!

🔗 www.youtube.com/watch?v=KiA9...

#VESS #VariantEffects #DropSynth

Next up! Two applications of MAVE!
🧬 DNA damage repair & cancer: Shelby Hemker (@jacobkitzman.bsky.social lab UMich) measures DNA repair of MUTYH variants.
🦠Antibiotic resistance: @kroman.bsky.social (‪@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.

Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning DropSynth technology enables scalable and cost-effective exploration of antibiotic resistance across the DHFR protein family.

Our new Science Advances paper is out! 🎉 The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. 👉 www.science.org/doi/10.1126/...

Save 90% on DNA variant libraries today YouTube video by IndieBio

We unlock breakthrough insights by delivering the best training sets in biology—at unprecedented scale. youtu.be/KuCaJTPHM2o

s2: permafrost thaw podcast is available for listening on Apple Podcasts, Spotify, Overcast, and through the Public Radio Exchange (PRX) ​ ​ or listen below. Press "More Info" in each episode player to reveal show...

Permafrost thaw takes center stage on Ice & Fire Climate Change podcast! 🎙️❄️🔥

S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research!

Tune in: iceandfireclimatepodcast.weebly.com/s2-permafros...

#ClimateChange #PermafrostResearch #SciComm

New preprint! We carried out the largest complementation and inhibition study for diverse homologs in a single protein family (DHFR) to date. Highlights thread follows... 1/n 🧵