One concept. One minute. Better #microscopy.

MDIBL Light Microscopy Facility
just launched Light Microscopy Fundamentals—a new YouTube series breaking down the basics of light microscopy in quick, bite-sized videos.

#microscopymonday #fluorescentproteins #lightsheet #SciEd #AcademicSky 🧪 🍎

Today's #microscopymonday of a zebrafish’s regenerating kidney thanks to Caramai Kamei and Iain Drummond at MDI Bio Lab. A new filtration unit, the nephron, forms from cells aggregating directly on an old tubule.

#microscopy #fluorescentproteins #lightsheet 🧪 🐟🎸 🍎 👩‍🔬 🌎 🖥️ 🧬

Join us in hearing from Hannah Somers, Digital Image Analyst at MDI Bio Lab's MDIBL Light Microscopy Facility, this Friday, March 6!

Get the details here: www.bioimagingnorthamerica.org/events/aimm-user-group/

#microscopy #fluorescentproteins #lightsheet 🧪 BioImaging North America

New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of #FluorescentProteins #ProteinEngineering #MachineLearning #SyntheticBiology 1/n

This amazing #microscopymonday capture of a young African turquoise killifish's brain blood vessels by the MDI Bio Lab's Haller lab was imaged using the MesoSPIM in the MDIBL Light Microscopy Facility.

#researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🐟🎸 🍎 👩‍🔬 🌎 🖥️ 🧬

Maine Microscopy: Foundations and Fundamentals 2026

👉 Registration now open!
Maine Microscopy: Foundations and Fundamentals
August 4–12, 2026

This course helps researchers build strong foundations in experiment planning, imaging and analysis—supporting rigor, reproducibility & better science.

#microscopy 🧪 🍎 #fluorescentproteins #lightsheet

A #fluorescencefriday image of an adult #zebrafish kidney by the Drummond Lab's Caramai Kamei, Ph.D.

We are looking at the aorta that runs through the kidney. Blood vessels connect to glomeruli, which hang like fruits on a tree.

#researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🤝

💫🧬🧫Fluorescent proteins make the holidays!🧫🧬💫

The Roger Y. Tsien lab (UCSD) made the wreaths with fluorescent proteins!🖌️🎨

#FluorescenceFriday #SciArt #AgarArt #MicrobialArt #BioArt #FluorescentProtein #FluorescentProteins

This #microscopymonday image by MDI Bio Lab's Travis Carney shows development of germ cells (cyan) in a fruit fly testis as they develop into sperm. A cell adhesion molecule (red) marks an epithelium at the base of the testes, while DNA is marked in green.

🧪 🤝 #fluorescentproteins #lightsheet

Congrats to Frederic Bonnet, MDIBL Light Microscopy Facility director—1st place winner in the BioImaging North America 2025 Light Microscopy Image Contest!

mdibl.org/frederic-bonnet-wins-fir...

#microscopymonday #fluorescentproteins #lightsheet 🧪 🤝

No tricks—just #fluorescent treats! 🎃

Captured by Romain Menard in the @mdibl-lmf.bsky.social, this #zebrafish shows its vascular (Fli1a:GFP) and motor neuron (mnx1:mCherry) systems glowing under the microscope.

#fluoresencefriday #microscopymonday #fluorescentproteins #lightsheet 🧪 🤝 🐟🎸

New #Methods article surveys #photophysical and spectral properties of popular #pH-sensing green fluorescent proteins, #pHluorins, including a new member pHluorin4, providing insights to optimize applications in different systems.
doi.org/10.14814/phy...
#GFP #FluorescentProteins #CellPhysiology

Thanks go out to MDI Bio Lab Digital Image Analyst @hsomers.bsky.social for this #microscopymonday view of an adult #zebrafish (brain vasculature) from the MesoSPIM.

#standwithscience #researchmatters 🧪 🤝 🐟🎸 #microscopy #fluorescentproteins #lightsheet

This #fluorescencefriday image from the @mandotar.bsky.social lab shows the innate immune system (red neutrophils and green macrophages) of a 5-day-old #zebrafish larva, captured by research assistant Janelle Grendler.

#researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🤝 🐟🎸

Four structures of rsCherry exhibiting varying degrees of degradation indicate that oxygen-induced degradation of rsCherry predominantly affects the chromophore without altering the protein backbone #FluorescentProteins #CrystalStructure #Degradation doi.org/10.1107/S205...

star fish under a microscope

A beautiful #microscopymonday sea star captured by @hsomers.bsky.social in 2022.

Sample: Asterias amurensis (Northern Sea Star)
Label: Nuclear staining with DAPI in Cyan and auto-fluorescence in green

#standwithscience #researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🤝 #wildlife

GFP+ stem cells moving through the complex environment of a zebrafish kidney.

This #microscopymonday image shows GFP+ stem cells moving through the complex environment of a #zebrafish kidney.

It was captured in 2023 by Senior Research Scientist Caramai Kamei.

#standwithscience #researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🤝 🐟🎸

Images created using our DNA-PAINT Kits for GFP, ALFA, mEOS, and RFP protein tag labeling. GFP - Nup96, ALFA - vimentin, mEOS - Tom20, RFP - EGFR. Credit for the beautiful ALFA-tag image goes to the Christophe Leterrier lab.

🚀𝗔𝗹𝗿𝗲𝗮𝗱𝘆 𝘄𝗼𝗿𝗸𝗶𝗻𝗴 𝘄𝗶𝘁𝗵 𝗽𝗿𝗼𝘁𝗲𝗶𝗻-𝘁𝗮𝗴𝗴𝗲𝗱 𝗰𝗲𝗹𝗹 𝗹𝗶𝗻𝗲𝘀? Take your imaging to the nanoscale with our 𝗽𝗹𝘂𝗴-𝗮𝗻𝗱-𝗽𝗹𝗮𝘆 𝗗𝗡𝗔-𝗣𝗔𝗜𝗡𝗧 𝗸𝗶𝘁𝘀 - designed to fit seamlessly into your existing immunostaining workflows.

www.massive-photonics.com/product-cate...

#DNAPAINT #SuperResolution #FluorescentProteins #SMLM

This colorized #microscopymonday image from 2023 by Frederic Bonnet shows kidney cells of a pig: the nuclei in yellow, the cytoplasmic vesicle in green and the membrane in magenta.

#standwithscience #researchmatters @mdibl-lmf.bsky.social

#microscopy #fluorescentproteins #lightsheet 🧪 🤝 🐟🎸 🍎 👩‍🔬

🎉 Last week, our colleague Liher García González from the Biomolecular Nanotechnology Laboratory at CIC biomaGUNE, defended his PhD thesis under the supervision of Dr. Aitziber L. Cortajarena.

👏 Congratulations, Dr García!

#PhdDefense #FluorescentProteins #CICbiomaGUNE

"Squid creatures" by Marko Pende, 2023

This Hawaiian bobtail squid and longfin inshore squid tissue was cleared with the DeepClear method, captured via #lightsheet microscope and 3D rendered using AMIRA.

#microscopymonday #standwithscience #researchmatters #fluorescentproteins 🧪 🤝 🔬

Curious about tagging an endogenous protein-of-interest 🧪 in vivo 🐁 - have you considered tissue-specific effects on levels & function? Read on... great skeetorial from @andrewwood.bsky.social @mrcmousenetwork.bsky.social #Degrons #FluorescentProteins #ProximityProteomics

📄 A preprint article introduces a set of far-red #FluorescentProteins (mfRFP, mfRFP-A, and mCardinal-A) with similar excitation/emission spectra and show how differences in photobleaching rate can be used to unmix their signal when imaged simultaneously: bit.ly/4kyp7aj

#Microscopy #Imaging

This #microscopymonday image by Assistant Professor Prayag @murawalalab.bsky.social is of a juvenile axolotl. It shows transgenic line labeling of the central and peripheral nervous system through a stereoscope.

#standwithscience #researchmatters #microscopy #fluorescentproteins #lightsheet 🧪 🤝

🎨🖌️🧫🧬🦠Smurfette painted with E. coli expressing EGFP (yellow) or small Ultra-Red Fluorescent Protein (cyan).🦠🧬🧫🖌️🎨
The name smURFP is inspired by the purified protein.
#FluorescenceFriday #SciArt #AgarArt #SciComm #FluorescentProtein #FluorescentProteins #smURFP

Bridge to a brighter future in fluorescence imaging - Nature Methods Bredt’s rule-inspired, bridged bicycle-strengthened fluorescent dyes resist both photobleaching and photoblueing, offering a powerful new toolkit for super-resolution fluorescence microscopy. This inn...

📜 Bridge to a brighter future in fluorescence imaging

🧑‍🔬 Frederik Brøndsted & Lei G. Wang

📔 @natmethods.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #Fluorophores #FluorescentProteins #Microscopy #BioImaging #FluorescenceMicroscopy #Imaging #CLSM #FluorescenceImaging

This week's #microscopymonday image depicts the blood vessels in a kidney organoid, at the 60x objective on the @healthcare.nikon.com spinning disk.

📸 @mdibl-lmf.bsky.social

#standwithscience #microscopy #fluorescentproteins #lightsheet 🧪 🤝

Caloric restriction is a well-established mechanism for improved protein recycling and increase longevity in #Celegans & other model organisms. This #microscopymonday image shows a C. elegan with marked germ granules.

#standwithscience 🧪 🤝 #microscopy #fluorescentproteins #lightsheet

This #microscopymonday video is of a #zebrafish embryo. It was imaged live, allowing researchers to investigate the impact of stress on #neutrophils (a type of white blood cells that play a crucial role in the body's #immunesystem) over time.

#standwithscience 🧪 🤝 #fluorescentproteins #lightsheet

Bright and photostable yellow fluorescent proteins for extended imaging - Nature Communications Yellow fluorescent proteins (YFPs) photobleach rapidly, restricting microscopy experiments. Here, the authors report mGold2s and mGold2t, YFPs that extend imaging durations up to 25 times longer than ...

📜 Bright and photostable yellow fluorescent proteins for extended imaging

🧑‍🔬 Jihwan Lee, Shujuan Lai, François St-Pierre, et al.

📔 @naturecomms.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #Microscopy #Imaging #BiologicalImaging #BioImaging #Fluorophores #FluorescentProteins #YFP