Day 3 begins our dive into #fluorscence #microscopy with a lab and lecture from none other than Dr. Wendy Salmon, @fishcscells.bsky.social, director of the Hooker Imaging Core @UNC. She teaches us about the fluorescence #light path, filters, and #fluorophores!

Apply here- bit.ly/49YQl7q

Spooky news: our Flash Sale to get 20% off Live-or-Dye™ ends in a week!
Shop Now: biotium.com/technology/f...?
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#labsale #cellviability #labhelp #sciencesale #microscopy #flowcytometry #deadcells #fluorophores #lablife #labcompany #apoptosis

Our Live-or-Dye™ October Sale is on!
Apply promo code TODYEFOR at checkout to get 20% off your next Live-or-Dye™ order of $250 or more.
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Shop here! biotium.com/technology/f...

#labsale #cellviability #labhelp #sciencesale #microscopy #flowcytometry #deadcells #fluorophores #lablife

a cartoon of alice from alice in wonderland with the words " and it begins " below her ALT: a cartoon of alice from alice in wonderland with the words " and it begins " below her

Trying to pinpoint the exact cause, I chased #autofluorescence down a rabbit hole. By the time I emerged, I looked like the mad hatter, but had also created my first #rstats package Luciernaga. It automated identifying degrading #tandem #fluorophores, and isolating secondary autofluorescences . 7/n

Check out our guide on popular #fluorophores for #dSTORM imaging! 📄🔬

We've compiled a handy PDF table that compares the most commonly used fluorescent labels, and other dyes for dSTORM imaging.

Download now -
hubs.li/Q03wfcjk0

#SuperResolution #Microscopy #FluorescenceMicroscopy #SingleMolecule

Bridge to a brighter future in fluorescence imaging - Nature Methods Bredt’s rule-inspired, bridged bicycle-strengthened fluorescent dyes resist both photobleaching and photoblueing, offering a powerful new toolkit for super-resolution fluorescence microscopy. This inn...

📜 Bridge to a brighter future in fluorescence imaging

🧑‍🔬 Frederik Brøndsted & Lei G. Wang

📔 @natmethods.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #Fluorophores #FluorescentProteins #Microscopy #BioImaging #FluorescenceMicroscopy #Imaging #CLSM #FluorescenceImaging

📄 A recent review article covers the latest in next-generation #Fluorophores and site-specific labeling strategies for different types of biomolecules, including info on their application in areas such as #SuperResolution #Microscopy: bit.ly/3Fhbeh9

#Fluorescence

𝗜𝗻𝘁𝗲𝗿𝗲𝘀𝘁𝗶𝗻𝗴 𝗦𝗰𝗶𝗲𝗻𝘁𝗶𝘀𝘁𝘀 𝘁𝗼 𝗞𝗻𝗼𝘄 𝗮𝗯𝗼𝘂𝘁 𝗡𝗼. 𝟭:

𝗗𝗼𝘂𝗴𝗹𝗮𝘀 𝗣𝗿𝗮𝘀𝗵𝗲𝗿

The man who cloned GFP (and Aequorin...), but fell into obscurity.

#Microscopy #Imaging #BioImaging #Fluorophores

A highly stable monomeric red fluorescent protein for advanced microscopy - Nature Methods The red fluorescent protein mScarlet3-H is bright, photostable and very robust to high temperature, chaotropic conditions and oxidative environments. mScarlet3-H works well in correlative light and el...

📜 A highly stable monomeric red fluorescent protein for advanced microscopy

🧑‍🔬 Haiyan Xiong, Qiyuan Chang, Jiayi Ding, Shuyuan Wang,
Wenhao Zhang, Yu Li, Yaochen Wu, Congxian Wu, Qingbing Zheng, Kiryl D. Piatkevich, Zhifei Fu, et al.

📔 @naturemethods.bsky.social

#️⃣ #Microscopy #Fluorophores

Bright and photostable yellow fluorescent proteins for extended imaging - Nature Communications Yellow fluorescent proteins (YFPs) photobleach rapidly, restricting microscopy experiments. Here, the authors report mGold2s and mGold2t, YFPs that extend imaging durations up to 25 times longer than ...

📜 Bright and photostable yellow fluorescent proteins for extended imaging

🧑‍🔬 Jihwan Lee, Shujuan Lai, François St-Pierre, et al.

📔 @naturecomms.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #Microscopy #Imaging #BiologicalImaging #BioImaging #Fluorophores #FluorescentProteins #YFP

Join us for a FluoroFinder Webinar! Learn how to optimize your Flow Cytometry experiments.

📅 April 16, 2025
📍 Online, 1:00 CT
🔗Register today: go.unl.edu/r4bt

#FluoroFinder #FlowCytometry #Fluorophores #ResearchTools #FlowPanelDesign #ScienceWebinar

A green lifetime biosensor for calcium that remains bright over its full dynamic range

📜 A green lifetime biosensor for calcium that remains bright over its full dynamic range

🧑‍🔬 Franka H van der Linden, @joachimgoedhart.bsky.social, et al.

📔 @elife.bsky.social

🔗 elifesciences.org/reviewed-pre...

#️⃣ #Microscopy #Calcium #Biosensor #Bioimaging #Fluorophores

The year may be winding down, but savings on the reagents you need are ramping up! Save big with free standard shipping on all #fluorescentdyes in December. Just use the code SHIPFREE24 at checkout.

More details on Vector #fluorophores & this promotion here: bit.ly/3ZcGnsf

Mimicking fluorophores from nature to generate artificial fluorescent proteins and biosensors - Nature Chemistry The miniaturization and redesigning of proteins to bear fluorescence signals can broaden their scope for potential applications. Now, one can construct a range of compact artificial fluorescent protei...

"Mimicking fluorophores from nature to generate artificial fluorescent proteins and biosensors"

www.nature.com/articles/s41...

Chia-Heng Hsiung & Xin Zhang for @naturechemistry.bsky.social.

Original article:
www.nature.com/articles/s41...

#FluorescenceMicroscopy #Micropscopy #Fluorophores

Making an impact with fluorescent probes - Nature Methods For Nature Methods’ 20th anniversary, our current and past editors reminisce about their favorite papers, initiatives and projects at the journal.

📜 Making an impact with fluorescent probes

🧑‍🔬 @ritastrack.bsky.social

📔 @natureportfolio.bsky.social Methods

🔗 www.nature.com/articles/s41...

#️⃣ #Microscopy #FluorescentProbes #Fluorophores #LightMicroscopy #FluorescentProteins

How to keep the lights on: the mission to make more photostable fluorophores Fluorescent labels that have greater resistance to bleaching could help researchers to get more from biological imaging. Fluorescent labels that have greater resistance to bleaching could help researc...

📜 How to keep the lights on: the mission to make more photostable fluorophores

🧑‍🔬 Ariana Remmel

📔 @natureportfolio.bsky.social

🔗 www.nature.com/articles/d41...

#️⃣ #Fluorophores #FluorescentProteins #Microscopy #PlantMicroscopy

PNAS Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Have you ever wondered why sea creatures even need #FluorescentProteins in the first place?

Cool paper by D. Nathaniel Clarke, Dimitri D. Deheyn, et al., showing that in an anemone, alleles of a Neon FP control not only color variations, but also serve as antioxidants 😲

#Microscopy #Fluorophores
👇

Now also with a commentary from @joachimgoedhart.bsky.social and Dorus Gadella: 👇

"Breaking up the StayGold dimer yields three photostable monomers"

www.nature.com/articles/s41...

#Microscopy #Imaging #FluorescentProteins #Fluorophores

A series of spontaneously blinking dyes for super-resolution microscopy bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 A series of spontaneously blinking dyes for super-resolution microscopy

🧑‍🔬 Katie L. Holland, Sarah E. Plutkis, Timothy A. Daugird, Abhishek Sau, Luke D. Lavis, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #Microscopy #Fluorophores #SuperResMicroscopy #CellBiology