Using #SingleMolecule imaging we find that TFs:

-boost co-transcriptional #ribosomal #RNA processing!

-their binding #dynamics fundamentally differ for mRNA (transient) versus rRNA #transcription (stable)!

@biorxivpreprint.bsky.social Anastasiia Chaban @embl.org

www.biorxiv.org/content/10.6...

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature Cas9 structures explain topology sensing and off-target activation.

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵

🔗 www.nature.com/articles/s41...

#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

Validate User

A bit less recent work published in @narjournal.bsky.social led by Klein, Das and Bera. Great collaboration with many labs showing that SARS-CoV-2 polymerase must undergo an activation step to enable RNA synthesis.
doi.org/10.1093/nar/...
#SARSCoV2 #polymerase #RNAvirus #singlemolecule

TAV2b Peptide Derivatives Underwind and Stabilize Double-Stranded RNA upon Binding Double-stranded RNA (dsRNA) has become an essential tool to understand biological processes with promising therapeutic implications. However, its usage is often limited due to poor cellular uptake and...

Recent article from the lab published in @jacs.acspublications.org. Big congrats to first author Zainab Rashid for leading the study and great collaboration with @tomngrossmann.bsky.social and Jocelyne Vreede labs!😃
doi.org/10.1021/jacs...
#singlemolecule #dsRNA #RNAtherapeutics

It's been a privilege to be involved with this. #FRET approaches have so many potential applications for linking in vitro studies with in cell behaviours, but lacked a consistent benchmarking molecule that works across systems: not any more! #IDRs #smFRET #singlemolecule

In the context of carboxysome positioning, @azaldegui.bsky.social found that the aggregation of a bacterial microcompartment is prevented by a self-organizing DNA-binding ATPase! #SingleMolecule #SuperResolution #Microscopy
with @cellforganized.bsky.social and JianLiu
doi.org/10.64898/202...

More peptides, better insights. The latest improvements in Quantum-Si's workflow – including recognizers, peptidases, and bioinformatics – offer an 85% increase in sequenceable peptides, giving you more coverage and more data with higher confidence.

#QSI #SingleMolecule #AminoAcids #Proteomics

Want to add easy access to #smFRET, #FCS and #FCCS to your core facility? Our new tools for single-molecule measurements offer:

💪 Reliable performance
⚡ Intuitive workflows
🦶 Small footprint, big impact

Learn more and get in touch > buff.ly/q8KQw0i

#corefacilities #biophysics #singlemolecule

More great #bps2026 #SingleMolecule science on Monday! Sam’s poster (B393) identifies IDR function in HP1 protein homologs, and Xiaofeng’s poster (B178) describes combining 2D tracking and 3D simulations to measure how the nucleoid viscosity responds to cellular processes!

Stop by Booth 508 at #BPS2026 and chat with us about the newfeatures of the 3rd generation RM21 MicroMirror TIRF Microscope. High signal-to-noise ratio, adaptability for a variety of single molecule techniques, streamlined alignment process. #singlemolecule #biophysics

If you are around #bps2026 on Monday afternoon @biophysicalsoc.bsky.social come chat with me about the mechanobiology of bacterial adhesins probed by #singlemolecule and #MD simulations !

Lots of great #SingleMolecule science at #bps2026! Check out Ruochuan’s poster (B431) on Sunday—she is understanding bacterial warfare by investigating how bacteriocin toxins translocate into E. coli.

🚀 Big news from @timcraggs.bsky.social x #BPS2026: meet the new EI-FLEX Pro! Automated alignment + focus, real-time analysis with publication-ready plots, modular upgrade to 96/384 wells, no darkroom or optical table, compact benchtop. Meet us at booth 401 or get in touch for a demo! #singlemolecule

Nautilus expands single-molecule proteomics into Parkinson’s New $1.6m collaboration aims to detect Parkinson’s disease earlier by tracking proteins one molecule at a time.

New $1.6m collaboration aims to detect Parkinson’s disease earlier by tracking proteins one molecule at a time.

vist.ly/4rs3p

#longevity #Proteomics #ParkinsonsResearch #EarlyDetection #PrecisionMedicine #SingleMolecule #Neurodegeneration #BiotechCollaboration

What better way to start your day than by exploring the key components of a confocal FRET experiment that ensure single-molecule sensitivity and accurate determination of FRET efficiencies? > buff.ly/NMausGH #singlemolecule #FRET #molecularbiophyics #biochemistry #molecules

Time-Dependent Structure Assessment of Conjugated Polymer Aggregates in Solution by Single-Molecule Fluorescence Spectroscopy Monitoring and understanding the aggregation kinetics of n-type polymers provide strategies to favorably control aggregation and thereby optimize conjugated polymers ink shelf life, printability, and thin-film properties. Here, the in situ characterization of n-type copolymer aggregates employing ensemble absorbance and fluorescence spectroscopy for spectral characterization, in combination with single-molecule fluorescence spectroscopy methods to identify subcategories of aggregates, is reported. Specifically, we utilize a diffusion-based single-molecule burst method that resolves individual aggregates as they traverse the observation volume, allowing us to determine the aggregate size, concentration, and chain conformation through the statistical analysis of single-aggregate fluorescence data. Base-stable P(EO-NDIT2) with branched ether-based side chains self-assembles from molecularly dissolved chains into nano- to micrometer-sized aggregates over the course of weeks to months, depending on the solvent used. Through spectral decomposition and polarization-sensitive single-molecule fluorescence spectroscopy, the aggregates were categorized by their size into small (Rh approximately 60 nm) and large (Rh approximately 300 nm) aggregates and monitored with time. An increase in size was correlated with enhanced fluorescence brightness and red-shifted emission as well as an increase in internal order, as revealed by emission anisotropy. This increase in order within the aggregates may be related to alignment of crystalline domains and a planarization of the polymer backbone torsion.

New publication in ACS Macromolecules highlighting extraordinary long time aggregation processes over months in conjugated polymers.

#ChemSky #OrganicElectronics #SingleMolecule #Fluorescence

doi.org/10.1021/acs....

Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo Kinesin-1 is an essential anterograde microtubule motor protein. The core kinesin motor is a homodimer of two heavy chains; N-terminal motor domains hydrolyse ATP and walk along microtubules, whilst a...

Congratulations to Alison Twelvetrees, Evelyn Smith and team at the University of Sheffield on their publication in iScience! - www.cell.com/iscience/ful...

Amazing to see the in-cell labelling and smFRET measurements in cell lysate.

#smfret #celllysate #singlemolecule #proteins

☕ Grab a cup of coffee and take 5 minutes to read the latest edition of our newsletter > 26707290.hs-sites-eu1.com/-temporary-s...

Subscribe to never miss a future edition > share-eu1.hsforms.com/2tB-S9AikSdG...

#biophysics #biochemistry #singlemolecule

Quantitative Mapping of the Lipid Nanoenvironment around Transmembrane Proteins in Living Cells Weak and transient lipid–protein interactions are thought to shape plasma membrane organization and function but have largely eluded experimental characterization. While model systems can only capture...

...hence challenging the functional role of boundary lipids. Using a #micropatterning approach combined with #singlemolecule #tracking they could not observe the presence of tightly associated boundary lipids for 3 out of 4 studied proteins. Open access here: pubs.acs.org/doi/10.1021/...

First test of live-cell single-molecule imaging in a new lab and it looks very promising!!!

Thanks to @ceitec-cellimcf.bsky.social instruments at
@ceitec.eu and @masarykuniversity.bsky.social. 🙌

#microscopy #singlemolecule #livecell #newlab

We will be at #SiMBio2026 (IIT Hyderabad), Jan 19–23!
Visit our booth to meet our experts.

Don’t miss Benjamin’s talk on Abbelight nanoscopy + spatial proteomics (Jan 20, 3:40 pm-3:55 pm).

Read more: simbio2026.com

#SingleMolecule #SMLM #SpatialProteomics

Biology happens when molecules interact - in solution, in motion, in fleeting moments. Freely diffusing smFRET captures these dynamics with nanometre precision. Our new practical #smFRET handbook distils principles & analysis for confident, in-solution data. 📘 buff.ly/AgGQCLY #singlemolecule

Also submitted just before the holidays: David Fuller collaborated with the Xindan Wang Lab to measure how growth-phase-dependent chromosome reorganization affects the behavior and function of bacterial nucleoid-associated proteins. #SingleMolecule #Microscopy
www.biorxiv.org/content/10.6...

Metastable Folding of Bacillus subtilis glmS Ribozyme Modulates Turnover by RNase J1 Rapid turnover of glmS mRNA in Bacillus subtilis by 5′–3′ exoribonuclease RNase J is essential for feedback regulation of glucosamine-6-phosphate (Glc…

Our recent #singlemolecule study on RNase J decay is out! Congrats, Yuan! 🎉🎉 www.sciencedirect.com/science/arti...

#BioSoft Seminar
Jan 7 | 11:00| Kaplun #118
Dr. Ivan Terterov (WIZ)
Correlation functions for stochastic processes with biased observations
Single-molecule FRET, photon detection & recovering true dynamics from biased data. Students & researchers welcome!
#TAU #Biophysics #SingleMolecule #FRET

Submitted just before the holidays: Dan Foust collaborated with the Lyle Simmons Lab to measure how enzyme activity is spatially modulated to process RNA-DNA hybrids. #SingleMolecule #Microscopy
www.biorxiv.org/content/10.6...

Text over a Christmas tree detail

May your science shine bright this time of year, and the next✨.

Happy holidays to the #singlemolecule community here on Bluesky, from everyone at LUMICKS.

A painless nerve growth factor variant uncouples nociceptive and neurotrophic TrkA signaling Nerve growth factor (NGF) binding to the receptor tyrosine kinase, TrkA, drives neurotrophic signaling essential for neuronal development and survival. This interaction simultaneously drives periphera...

A preprint from my lab @yalepharm.bsky.social: doi.org/10.1101/2025...
show molecular mechanisms of uncoupling nociceptive and neurotrophic TrkA signaling, using #singleMolecule #microscopy, #cryoEM, #electrophysiology, #signaling, offering a clear blueprint to target this long sought-after pathway!