Beautiful work showing the power of #smFRET for uncovering the complexities ensembles in biology, this time for #RNA structures during transcription- congratulations @olivierduss.bsky.social !!

🙌 Congratulations to Chadda et al. on their new paper! #smFRET on the EI-FLEX provided a highly complementary technique alongside SEC, mass spectrometry, mass photometry & bulk FRET to elucidate the pathway that forms mature intasomes from viral #DNA & retrovirus integrase dimers.👀 > buff.ly/mkBUmjc

Nice overview of another cool #smFRET study, this time unpicking protein folding dynamics @timcraggs.bsky.social @ei-science.bsky.social

It's been a privilege to be involved with this. #FRET approaches have so many potential applications for linking in vitro studies with in cell behaviours, but lacked a consistent benchmarking molecule that works across systems: not any more! #IDRs #smFRET #singlemolecule

Want to add easy access to #smFRET, #FCS and #FCCS to your core facility? Our new tools for single-molecule measurements offer:

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#corefacilities #biophysics #singlemolecule

Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo Kinesin-1 is an essential anterograde microtubule motor protein. The core kinesin motor is a homodimer of two heavy chains; N-terminal motor domains hydrolyse ATP and walk along microtubules, whilst a...

Congratulations to Alison Twelvetrees, Evelyn Smith and team at the University of Sheffield on their publication in iScience! - www.cell.com/iscience/ful...

Amazing to see the in-cell labelling and smFRET measurements in cell lysate.

#smfret #celllysate #singlemolecule #proteins

Want to add fast, solution-based #single-molecule dynamics to your workflow? Our new, practical handbook shows how confocal #smFRET reveals conformational change, heterogeneity & nanoscale distances, with clear, step-by-step guidance from basics to advanced analysis > buff.ly/xHefIrP

Biology happens when molecules interact - in solution, in motion, in fleeting moments. Freely diffusing smFRET captures these dynamics with nanometre precision. Our new practical #smFRET handbook distils principles & analysis for confident, in-solution data. 📘 buff.ly/AgGQCLY #singlemolecule

Partial wrapping of single-stranded DNA by replication protein A and modulation through phosphorylation Abstract. Single-stranded DNA (ssDNA) intermediates which emerge during DNA metabolic processes are shielded by replication protein A (RPA). RPA binds to s

Curious how single-molecule tools are transforming our understanding of DNA? Chadda et al. used smFRET and DEER to reveal that RPA partially wraps single-stranded DNA instead of simply stretching it - academic.oup.com/nar/article/...
#DNA #smFRET #molecularbiology #proteinDNAinteractions

smFRET tracks one molecule at a time to reveal real low/high-FRET populations, dynamic transitions, and nanoscale changes that traditional FRET masks. Learn more - bit.ly/44TVTwy #smFRET #biophysics #singlemolecule

It's Christmas in advance in the lab, as we recieved the first ever #Luminosa from @picoquant.bsky.social to be installed in France. It will be available on the PIBBS facility, as a combo with a Brucker Atomic force microscope for #mecanobiology, #smFRET, #FLIM, #FCS, #AFM applications and more.
🔬

Tim Craggs: Adding dynamics to structural biology | J. Heyrovsky Institute of Physical Chemistry

👋 Calling all Prague-based structural biologists and single-molecule enthusiasts! You are invited to listen and learn from @timcraggs.bsky.social on 9 Dec at the J. Heyrovský Institute of Physical Chemistry - www.jh-inst.cas.cz/seminar/tim-... #singlemolecule #structuralbiology #smfret #fcs

A DNA Scaffold Approach Facilitates 5′ Labeling of the SARS‐CoV‐2 RNA Pseudoknot for Single‐Molecule Förster Resonance Energy Transfer Investigation DNA scaffold-based labeling strategy is introduced that enables single-molecule Förster resonance energy transfer (smFRET) studies of the SARS-CoV-2 frameshifting pseudoknot. The smFRET measurements ...

Curious about new routes for labelling often complicated RNAs for single-molecule FRET studies? You'll want to check out Graham et al.'s recently published work in ChemPhysChem (congratulations 🥳) - buff.ly/SkaruHO
#rna #rnaresearch #virology #singlemolecule #smfret #nucleicacids

🔎 How does in-solution smFRET reveal the dynamic structure–function links in complex proteins?
Watch Prof. @shenk0128.bsky.social's seminar using the EI-FLEX to probe Rag GTPase conformations. Q&A covers labelling, HMMs & why solution-based smFRET matters.
youtu.be/2AfskEVyRCI #smfret #biophysics

Mechanistic insights into the activity of SARS-CoV-2 RNA polymerase inhibitors using single-molecule FRET Abstract. The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has resulted in significant global mortality, with over 7 million cases reported. Despite

Professor Robb says: "Our EI-FLEX platform has been central to understanding the molecular details of viral replication and drug action. It’s quickly become a core instrument across many aspects of our virology research." Read her paper here - doi.org/10.1093/nar/... #smFRET #drugdevelopment

📢 Our Exciting Seminars are back! Join @timcraggs.bsky.social & @shenk0128.bsky.social on Nov 20 for 'Mapping the global conformation of the Rag GTPase heterodimer with single-molecule FRET' > us06web.zoom.us/meeting/regi...

#biophysics #lifescienceevent #molecularmedicine #biochemistry #smfret

Oh this looks 🤩. #smFRET

Thanks for making all these efforts to outreach. In case anyone has questions, please do not hesitate to contact me. 📝🧑‍💻

The entrance lobby for the new Exciting Instruments facility - with cool 3D logos and reflections in the window an interesting way around!

Love the optics here - see the reflection!

We are welcoming a lot of people to @ei-science.bsky.social getting great data on their own biological systems for prelim data for grants.

It’s nice to have a greta space to welcome them to!

Is there an #smFRET experiment you’d like to try? Ping me here!

The Zuo1 C-terminal domain stabilizes DNA guanosine quadruplex (G4) structures located on Chromosome IX in Saccharomyces cerevisiae Abstract. Deoxyguanosine quadruplexes (G4s) form stable non-B-DNA structures that can affect transcription, replication, and genome stability. Depending on

Our work on DNA G4 interactions with Zuo1 in collaboration with @paeschkelab.bsky.social and the Penedo lab is out in @narjournal.bsky.social 😍

Full text: academic.oup.com/nar/article/...

#G4 #G-Quadruplex #NMR #smFRET #ChIP-qPCR

Average doesn't really exist... so why measure it?

Our compact and easy-to-use instrument has been designed to help researchers access dynamic single-molecule insights from their own lab benchtops.

Find out more about the EI-FLEX and book a demo bit.ly/3KZK3Ku

#singlemolecule #smfret #fcs

I am very much looking forward to an inspiring week at #Mifobio2025 - thanks for the invite! I am counting on learning new exciting (pun intended) insights on fluorescence and happy to share our experience and vision on large throughput #smFRET .

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study - Nature Methods A multi-laboratory study finds that single-molecule FRET is a reproducible and reliable approach for determining accurate distances in dye-labeled DNA duplexes.

Can smFRET deliver reproducible, absolute distance measurements?
Yes! Check out Hellenkamp et al.'s (2018) paper to learn more > www.nature.com/articles/s41...

#singlemolecule #smfret #biophyics #biochemistry

🚀 Our 'professor in a box', a quick-watch smFRET explainer video, a very exciting Chair appointment and more... see what we've been up to in September in the latest edition of our newsletter - buff.ly/EDA6U5s

#structuralbiology #moleculardynamics #singlemolecule #smfret #fcs #deeptech #startups

Rush! The application deadline for this #PhD or #Postdoc opportunity in my group is TODAY!
#smFRET #DyeCycling #nano-photonics & Co

Mechanistic insights into the activity of SARS-CoV-2 RNA polymerase inhibitors using single-molecule FRET Abstract. The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has resulted in significant global mortality, with over 7 million cases reported. Despite

🤔 How did Groves et al. use the FLEX to reveal insights about SARS-CoV-2’s RNA polymerase? Find out here > doi.org/10.1093/nar/...

Want to know more about the FLEX? Learn more, request a tech spec or book a demo on our website: excitinginstruments.com/ei-flex/?utm... #smfret #RNA #nucleicacids

👋 At the Midlands Biophysics Network meeting today? So are we!

Visit our stand to chat about how we're bringing solution-based single-molecule techniques right to your benchtop - no extensive training required.

#midlandsbiophysicsnetworkmeeting #biophysics #singlemolecule #smfret #fcs

Thrilled to see long-time #FRET experts moving into single-molecule work with our technology at McMaster University! Plus, our instrument now also supports University at Albany's core facility with #smFRET & #FCS! A very Exciting week! 🙌 #biophysics #singlemolecule #structuralbiology

Missing biomolecular dynamics at picomolar levels? The EI-FLEX makes single-molecule research quick and easy with precision and stability. Watch Dr. Quinn below and book a demo: excitinginstruments.com/contact-us/?...
#singlemolecule #structuralbiology #moleculardynamics #smFRET #FCS

Heading to the Austrian Cryo-EM Symposium in November? Let's catch up! We'll be on hand to share how freely diffusing smFRET can complement this already exquisitely detailed technique #cryoEM #smfret #singlemolecule

Nice use of #smFRET to study #idr #dynamics