Partial wrapping of single-stranded DNA by replication protein A and modulation through phosphorylation Abstract. Single-stranded DNA (ssDNA) intermediates which emerge during DNA metabolic processes are shielded by replication protein A (RPA). RPA binds to s

Curious how single-molecule tools are transforming our understanding of DNA? Chadda et al. used smFRET and DEER to reveal that RPA partially wraps single-stranded DNA instead of simply stretching it - academic.oup.com/nar/article/...
#DNA #smFRET #molecularbiology #proteinDNAinteractions