Fluorescence microscopy image of a spherical cluster of chloroplasts, showing magenta-stained interiors surrounded by bright green outlines against a black background.

Moss (Physcomitrium patens) protoplasts (wallless cells) transformed with chloroplast envelope protein tagged with mGFP (green). The chlorophyll autofluorescence is in magenta.
The protrusions of the chloroplast envelope are called stromules.
#microscopymonday, #plantcells, #plantmicroscopy, #moss

This should be super helpful for anybody working on #PlantCellWalls, particularly #Cellulose.

A step-by-step guide for quantitative(!) live-imaging of cellulose synthesis in planta.

#PlantScience #PlantMicroscopy #QuantitativeImaging #Bioimaging #PlantDevelopment

👇👇👇

Fluorescent microscope image showing a dense network of bright green, irregularly shaped plant cells with glowing outlines against a dark background.

Surface (epidermal) cells of many plants are shaped like jigsaw puzzle pieces. 🧩
These are epidermal cells of tobacco (Nicotiana benthamiana) expressing cytosolic EGFP. The bright spherical structures are cell nuclei.
#microscopymonday, #plantcells, #plantmicroscopy

The image shows large moss chloroplasts organized in irregular rows. The chloroplasts, in magenta, are somewhat rectangular in shape but with many irregularities. Many green tubular protrusions extend from the chloroplast surface.

This is what happens to the moss (Physcomitrium patens) chloroplasts when we grow cells on β-lactam antibiotics. β-lactams inhibit the synthesis of the peptidoglycan in the chloroplast envelope, leading to their dramatic expansion.
#microscopymonday #moss #plantcells #plantmicroscopy
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Image shows two elongated cells with rounded tips. The cell periphery and internal structures are visible in different shades of orange. Some internal structures are very small, while others are very big and occupy a large part of the cell. Small cyan-colored ovoid chloroplasts are dotted around within the cells.

During my postdoc, I looked at hundreds of images like this 🤩
These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan.
#microscopymonday #moss #plantcells #plantmicroscopy

The image shows several chloroplasts in green and magenta or both. In the center of the image, five chloroplasts are organized in a circle, and each is making a protrusion towards the center of the circle. The center of these chloroplasts is in magenta, while the periphery and the protrusions are in green.

In preparation for Halloween, these chloroplasts had a seance! 👻🎃
These five Arabidopsis chloroplasts were extending their stromules (protrusions of the chloroplast envelope and stroma) towards each other.
#microscopymonday #Arabidopsis #plantcells #plantmicroscopy

This is a fantastic talk by Chris Hawes @nhm-london.bsky.social.

He speaks about #PlantMicroscopy in particular, but also the History of #Microscopy.

www.youtube.com/watch?v=eAFZ...

Have a look at this Obituary of Chris, too:
onlinelibrary.wiley.com/doi/10.1111/...

#PlantScience

Chloroplasts (spherical structures in magenta) are surrounded by membranes of vacuoles (in green).

I love this image of moss (Physcomitrium patens) chloronema cells, as you can see how chloroplasts (in magenta 🟣) indent the vacuole to make room for themselves. The vacuolar membrane is labeled with a GFP marker (green 🟢).
#microscopymonday #cellbiology #plantmicroscopy #plant #moss

The Arabidopsis thaliana–Fusarium oxysporum strain 5176 pathosystem: an overview This review provides an overview of the advances made with the Arabidopsis thaliana–Fusarium oxysporum5176 pathosystem in understanding the cause and defen

Here starts my scientific independence as ARC DECRA investigator in Melbourne.

I decided to use the Arabidopsis - #Fusarium Pathosystem to study #PlantImmunity with spatial resolution ( #SpatialImmunity ) using #PlantMicroscopy.

So, I wrote about my idea first. My next @jxbotany.bsky.social paper:

NO more hiding: whole‐plant live imaging of nitric oxide reveals systemic signalling Click on the article title to read more.

📜 NO more hiding: whole-plant live imaging of nitric oxide reveals systemic signalling

🧑‍🔬 John T. Hancock

📔 @newphyt.bsky.social

🔗 nph.onlinelibrary.wiley.com/doi/full/10....

#️⃣ #PlantScience #PlantImaging #PlantImmunity #NitricOxide #PlantMicroscopy

CarboTag: a modular approach for live and functional imaging of plant cell walls - Nature Methods A suite of nontoxic and permeable CarboTag probes in various colors enables live quantitative imaging of a range of plant cell wall characteristics and dynamic, high-resolution mapping of cell wall ch...

📜 CarboTag: a modular approach for live and functional imaging of plant cell walls

🧑‍🔬 Maarten Besten, Joris Sprakel, et al.

📔 @naturemethods.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantCellWalls #PlantTools #PlantMicroscopy #PlantImaging #PlantMethods

Quantifying Plant Biology with Fluorescent Biosensors | Annual Reviews Plant biology is undergoing a spatial omics revolution, but these approaches are limited to snapshots of a plant's state. Direct, genetically encoded fluorescent biosensors complement the omics a...

📜 Quantifying plant biology with fluorescent biosensors

🧑‍🔬 @botanicaljim.bsky.social, @maxjosse.bsky.social, Bijun Tang, @xanderjones.bsky.social

📔 @annualreviews.bsky.social

🔗 www.annualreviews.org/content/jour...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #Biosensors #PlantSensors

🌱 Helloooo Bluesky!
Excited to join the community and introduce MIAtecs! I help you unlock the full potential of your samples by providing consulting services in imaging, image analysis, and much more! 🔬🚀
Let’s connect! 😊
👉 www.miatecs.com

#PlantMicroscopy #Biology #MIAtecs

Atomic force microscopy imaging of plant cell walls A review of advancements in atomic force microscopy imaging of cell wall structure and analysis of the mechanical properties, specific binding sites, and s

📜 Atomic force microscopy imaging of plant cell walls

🧑‍🔬 Junbao Pu, Wenjun Zhang, et al.

📔 @plantphys.bsky.social

🔗 academic.oup.com/plphys/artic...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #PlantAFM #AtomicForceMicroscopy #PlantCellWalls

Making plant tissue accessible for cryo-electron tomography Cryo-Electron Tomography (cryo-ET) allows to visualize the molecular architecture of pristinely preserved cells and tissues. The workflow of sample preparation for cryo-ET is rather complex; it involv...

📜 Making plant tissue accessible for cryo-electron tomography

🧑‍🔬 Matthias Pöge1, @madic.bsky.social, Wolfgang Baumeister, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #CryoET

A tweet from Joachim Goedhart from August 24, 2018. It says "I was contacted for advice on setting up expts with a #FRET biosensor. Me: Can you think of a simpler experiment to address your question? Him: Yes. Me: That's my advice."

Every time someone mentions using a #FRET #biosensor to me, I still think about this tweet from @joachimgoedhart.bsky.social.

Even after seven long years.

#Microscopy #Imaging #CellBiology #PlantMicroscopy

The secreted redox sensor roGFP2-Orp1 reveals oxidative dynamics in the plant apoplast Specific generation of reactive oxygen species (ROS) is important for signalling and defence in many organisms. In plants, different types of ROS perform useful biological functions in the extracellul...

📜 The secreted redox sensor roGFP2-Orp1 reveals oxidative dynamics in the plant apoplast

🧑‍🔬 Julian Ingelfinger, Stefanie J. Müller-Schüssele, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantPhysiology #PlantImaging #PlantMicroscopy #PlantMethods

Super-resolution expansion microscopy in plant roots The development of expansion microscopy in the Arabidopsis root enabled the imaging of molecular targets in tissues at diffraction-unlimited resolution.

📜 Super-resolution expansion microscopy in plant roots

🧑‍🔬 Michelle Gallei, Sven Truckenbrodt, Johann G. Danzl, et al.

📔 @theplantcell.bsky.social

🔗 academic.oup.com/plcell/advan...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #PlantSuperRes

A Short History of Plant Light Microscopy When the microscope was first introduced to scientists in the 17th century, it started a revolution. Suddenly, a whole new world, invisible to the naked eye, was opened to curious explorers. In respo...

I never had a use for this image in any research paper, so eventually I used it in my short history of plant light microscopy, as it is one of very few examples of @henriqueslab.bsky.social & @superresolusian.bsky.social's #SRRF-microscopy applied in plant cells.
#PlantScience #PlantMicroscopy
👇

Kasper van Gelderen on stage at the Botanik Tagung

#BT2024DBG Cell Biology Session:
@kaspervangelderen.bsky.social spoke about photobody formation and stability. Really cool #PlantMicroscopy and #LiveImaging with red/far-red light switching. 👌

#PlantScience

Development of bright fluorescent auxin bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 Development of bright fluorescent auxin

🧑‍🔬 Tsuyoshi Aoyama, Yuichiro Tsuchiya, et al.

📔 @biorxivpreprint.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #FluorescenceMicroscopy #Phytohormones #PlantMicroscopy #PlantImaging #PlantScience

Probing plant signal processing optogenetically by two channelrhodopsins - Nature Using new optogenetic tools to induce distinct ion fluxes, a study shows that these discrete signals trigger different metabolic and transcriptional pathways that allow plants to respond to specific t...

📜 Probing plant signal processing optogenetically by two channelrhodopsins

🧑‍🔬 Meiqi Ding, Yang Zhou, Dirk Becker, Shang Yang, Rainer Hedrich, Georg Nagel, Shiqiang Gao, Kai R. Konrad, et al.

📔 Nature

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantMicroscopy #Optogenetics

Single-molecule analysis reveals the phosphorylation of FLS2 governs its spatiotemporal dynamics and immunity The phosphorylation of FLS2 may activate flg22-triggered immunity by facilitating the partitioning of FLS2 into functional AtRem1.3-associated nanodomains.

📜 Single- molecule analysis reveals the phosphorylation of FLS2 governs its spatiotemporal dynamics and immunity

🧑‍🔬 Yaning Cui, Hongping Qian, Jinxing Lin, et al.

📔 @elife.bsky.social

🔗 elifesciences.org/articles/91072

#️⃣ #PlantScience #PlantImmunity #PlantMicroscopy #PlantSpectroscopy

ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems

🧑‍🔬 Kevin L. Cox, Sarah A. Pardi, Kirk J. Czymmek, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantMicroscopy #ExpansionMicroscopy #PlantMethods

CarboTag: a modular approach for live and functional imaging of plant cell walls bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 CarboTag: a modular approach for live and functional imaging of plant cell walls

🧑‍🔬 Maarten Besten, Joris Sprakel, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantCellWalls #PlantImaging #PlantMicroscopy

How to keep the lights on: the mission to make more photostable fluorophores Fluorescent labels that have greater resistance to bleaching could help researchers to get more from biological imaging. Fluorescent labels that have greater resistance to bleaching could help researc...

📜 How to keep the lights on: the mission to make more photostable fluorophores

🧑‍🔬 Ariana Remmel

📔 @natureportfolio.bsky.social

🔗 www.nature.com/articles/d41...

#️⃣ #Fluorophores #FluorescentProteins #Microscopy #PlantMicroscopy

P4B: a novel probe to study cellulose synthesis and microtubule dynamics Nicola Trozzi; P4B: a novel probe to study cellulose synthesis and microtubule dynamics, Plant Physiology, , kiae305, https://doi.org/10.1093/plphys/kiae30

📜 P4B: a novel probe to study cellulose synthesis and microtubule dynamics

🧑‍🔬 Nicola Trozzi

📔 Plant Physiology

🔗 academic.oup.com/plphys/advan...

#️⃣ #PlantScience #PlantCellWalls #PlantMicroscopy #Microtubules #Cellulose

The AMSlide for noninvasive time‐lapse imaging of arbuscular mycorrhizal symbiosis The <em>Journal of Microscopy</em> is the oldest journal dedicated to the science of microscopy, publishing the latest advances in microscopy techniques and novel areas of application.

📜 The AMSlide for noninvasive time-lapse imaging ofarbuscular mycorrhizal symbiosis

🧑‍🔬 Jennifer McGaley, Ben Schneider, Uta Paszkowski

📔 Journal of Microscopy

🔗 onlinelibrary.wiley.com/doi/10.1111/...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #PlantSymbiosis #ArbuscularMycorrhiza #AMFungi

Label-free structural imaging of plant roots and microbes using third- harmonic generation microscopy bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 Label-free structural imaging of plant roots using three-photon autofluorescence and third-harmonic generation microscopy

🧑‍🔬 Daisong Pan, Jose A. Rivera, Na Ji, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #RootBiology

Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

📜 Simultaneous and Dynamic Super‐Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual‐color sptPALM

🧑‍🔬 Leander Rohr, Sven zur Oven‐Krockhaus, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantMicroscopy #Microscopy #SuperResMicroscopy