This should be super helpful for anybody working on #PlantCellWalls, particularly #Cellulose.

A step-by-step guide for quantitative(!) live-imaging of cellulose synthesis in planta.

#PlantScience #PlantMicroscopy #QuantitativeImaging #Bioimaging #PlantDevelopment

👇👇👇

Check out this wonderful 90 second video highlighting our group's research and teaching in the Penn State Eberly College of Science - a big thank you to Michelle Bixby and the ECOS communications office! #PlantCellWalls 🪴 🔬 🤓 www.youtube.com/watch?v=t0Dd...

Fig. 5 Reading out cell wall memories.

#Viewpoint: Memory in the wall: expanding our understanding of the roles of #PlantCellWalls

Shikata, Yoshinari, Asaoka & Takahashi 👇

📖 nph.onlinelibrary.wiley.com/doi/10.1111/...

#LatestIssue #PlantScience

The rice BRITTLE CULM 4 gene encodes a membrane protein affecting cellulose synthesis in the secondary cell wall Abstract. The formation of secondary cell walls, which provide mechanical strength to the plant body, depends on numerous factors. Studies on rice brittle

Standing tall...🌾

Yamaguchi et al. have identified a novel membrane protein (BRITTLE CULM 4) that affects cellulose composition in the secondary cell walls of #rice

📖 doi.org/10.1093/pcp/...

#PlantScience
#PlantCellWalls

If you're following the #Coffee #PlantCellWalls work from the labs of @zhengli.bsky.social & @icesarino.bsky.social (see quoted), then here's their latest paper:

👉 www.sciencedirect.com/science/arti...

"Polysaccharides of coffee"

#PlantScience

Establishment and functions of the Casparian strip

📜 Establishment and functions of the Casparian strip

🧑‍🔬 @swatimahiwal.bsky.social, @tonnigrubeandersen.bsky.social, Defeng Shen

📔 @mplantpcom.bsky.social

🔗 www.cell.com/molecular-pl...

#️⃣ #PlantScience #PlantDevelopment #CasparianStrip #PlantCellWalls #Nodules #RootBiology

Arrived together with @sara-morghen.bsky.social to the beautiful UBC campus in Vancouver for the Plant Cell Wall Conference 2025. Ready to discuss the relation between cell walls, water sensing, abscisic acid and mechanobiology. @hydrosensing.bsky.social #plantcellwalls

The role of VND transcription factors in xylem vessel development and secondary wall formation Transitioning from aquatic to terrestrial life required specialized vascular tissues, with xylem enabling water transport and structural support. Vascular-related NAC (NAM, ATAF1/2, CUC2) domain (VND...

📜 The role of VND transcription factors in xylem vessel development and secondary wall formation

🧑‍🔬 Ulrike Lehmann, @schneiderlab.bsky.social

📔 @newphyt.bsky.social

🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...

#️⃣ #PlantScience #PlantCellWalls #PlantDevelopment #PlantGenetics #Cellulose #Lignin

Linked in with lignin - Nature Chemistry Moses Dike and Shudipto Konika Dishari explore lignin’s historic journey alongside human civilization and showcase its game-changing potential to drive sustainability without compromising performance.

📜 Linked in with lignin

🧑‍🔬 Moses Dike, Shudipto Konika Dishari

📔 @natchem.nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #Lignin #PlantPhysiology #PlantCellWalls

MYB68 regulates radial endodermal differentiation and suberin patterning In this study, Kraska et al. identify MYB68 as a novel regulator of endodermal suberization, linked to the formation of distinct identities in the xylem-pole-associated endodermis. The research uncove...

📜 MYB68 regulates radial endodermal differentiation and suberin patterning

🧑‍🔬 @leoniekraska.bsky.social, @tonnigrubeandersen.bsky.social, et al.

📔 @cp-cellreports.bsky.social

🔗 www.cell.com/cell-reports...

#️⃣ #PlantScience #PlantDevelopment #Suberin #PlantCellWalls #PlantRoots #PassageCells

Imaging of specialized plant cell walls by improved cryo-CLEM and cryo-electron tomography Cryo-focused ion beam scanning electron microscopy (cryo-FIBSEM) has become essential for preparing electron-transparent lamellae from cryo-plunged and high-pressure frozen specimens. However, targeti...

📜 Imaging of specialized plant cell walls by improved cryo-CLEM and cryo-electron tomography

🧑‍🔬 Daraspe J., Bellani E., De Bellis D., @chgenoud.bsky.social, @nikogeldner.bsky.social

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantCryoEM #PlantCellWalls

Galactan mobilization during carbon starvation compromises cell wall-mediated fungal resistance in Arabidopsis Polysaccharides are the main components present in plant cell walls. They form a network that is dynamically modified during growth and upon both abiotic and biotic stress. We investigated how the cel...

📜 Galactan mobilization during carbon starvation compromises cell wall-mediated fungal resistance in Arabidopsis

🧑‍🔬 Louisa Reckleben, @beritebert.bsky.social, Timo Engelsdorf, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantImmunity #PlantCellWalls

I also found that the #PlantCellWalls field is not for me, so I decided to move on.
But not before writing a review on the field, this time as an "At a Glance" article for @jcellsci.bsky.social, so once again the Company of @biologists.bsky.social published my career-step-summary.

shorturl.at/TjtYz

Three petri dishes with Arabidopsis seedlings. In the plate labeled "Control", the seedlings stand upright. In the plate labeled "EBL", a brassinosteroid phytohormones, the plants are all flat on the surface. On the last dish, labeled "BRZ", an inhibitor or Brassinosteroids, they are short, but most stand up again.

But at least we got a short paper on #PlantCellWalls, #PlantGravitropism and #Phytohormones out in @plantcellphysiol.bsky.social.

shorturl.at/t69rU

Nonetheless, the support from the @dfg.de was fantastic (highly recomment their PostDoc Fellowship!), and I had a great time as postdoc in Australia.

Frontiers | Cell Wall Heterogeneity in Root Development of Arabidopsis Plant cell walls provide stability and protection to plant cells. During growth and development the composition of cell walls changes, but provides enough st...

I then moved to Melbourne, 🇦🇺, to study a link between #PlantCellWalls and #PlantDevelopment. An understudied field - still today.

I summarized my ideas in a review first:
www.frontiersin.org/journals/pla...

This @dfg.de funded project did NOT work as planned at all.

Here's an overview Thread 🧵 on my own research while at...

𝟮𝟬𝟬𝟳 - 𝟮𝟬𝟭𝟱 @hhu.de with @simonrdg.bsky.social

𝟮𝟬𝟭𝟲 - 𝟮𝟬𝟭𝟵 UniMelbourne with the ARC CoE #PlantCellWalls

𝟮𝟬𝟮𝟬 - 𝟮𝟬𝟮𝟯 UniMelbourne Group Leader

𝟮𝟬𝟮𝟯 - 𝟮𝟬𝟮𝟱 @mpipz.bsky.social / @ceplas.bsky.social with @tonnigrubeandersen.bsky.social

🧵 👇

The Cell Wall Controls Stem Cell Fate in the Arabidopsis Shoot Apical Meristem At the basis of plant developmental plasticity are continuously active pluripotent stem cells, which fuel the life-long post-embryonic formation of new organs. Since plant cells are encased in cell wa...

📜 The Cell Wall Controls Stem Cell Fate in the Arabidopsis Shoot Apical Meristem

🧑‍🔬 Tasnim Zerin, Paola Ruiz-Duarte, Ann-Kathrin Schürholz, Sebastian Wolf, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantCellWalls #PlantDevelopment

Modulation of the GT Family 47 clade B gene affects arabinan deposition in elaters of Marchantia polymorpha The plant cell wall polymer α-1,5-linked arabinan is associated with important functions in plant physiology, such as cell wall flexibility and desiccation tolerance in angiosperms. However, its biosy...

📜 Modulation of the GT Family 47 clade B gene affects arabinan deposition in elaters of Marchantia polymorpha

🧑‍🔬 @fkang.bsky.social, @beritebert.bsky.social, Staffan Persson, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1101/2025.05.02.651808v1

#️⃣ #PlantScience #PlantCellWalls

CarboTag: a modular approach for live and functional imaging of plant cell walls - Nature Methods A suite of nontoxic and permeable CarboTag probes in various colors enables live quantitative imaging of a range of plant cell wall characteristics and dynamic, high-resolution mapping of cell wall ch...

📜 CarboTag: a modular approach for live and functional imaging of plant cell walls

🧑‍🔬 Maarten Besten, Joris Sprakel, et al.

📔 @naturemethods.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantCellWalls #PlantTools #PlantMicroscopy #PlantImaging #PlantMethods

Establishing a suberin tool kit for determining suberization within classical and ‘orphan’ tissues Suberin is a complex biopolymer found in land plants that serves as a natural barrier, regulating water and nutrient uptake while also providing defense against invading pathogens. Structurally, suber...

📜 Establishing a suberin tool kit for determining suberization within classical and ‘orphan’ tissues

🧑‍🔬 Justin B. Nichol, Lorena S. Yeung, Mark A. Bernards, Marcus A. Samuel

📔 @cp-trendsplantsci.bsky.social

🔗 www.cell.com/trends/plant...

#️⃣ #PlantScience #Suberin #PlantMethods #PlantCellWalls

Mutations in HEADING DATE 1 affect transcription and cell wall composition in rice A zinc finger transcription factor that is central to photoperiodic flowering also plays a role in determining cell wall composition and abiotic stress res

📜 Mutations in HEADING DATE 1 affect transcription and cell wall composition in rice

🧑‍🔬 Marco Biancucci, Daniele Chirivì, Fabio Fornara, et al.

📔 @plantphys.bsky.social

🔗 academic.oup.com/plphys/advan...

#️⃣ #PlantScience #Rice #Agriculture #PlantCellWalls #PlantPhysiology

Scientists Witness Living Plant Cells Generate Cellulose and Form Cell Walls For the First Time | Newswise In a groundbreaking study on the synthesis of cellulose – a major constituent of all plant cell walls – a team of Rutgers University-New Brunswick researchers has captured images of the microscopic pr...

Scientists Witness Living Plant Cells Generate Cellulose and Form Cell Walls For the First Time
www.newswise.com/articles/sci...
#cellulose #biofuel #plantcellwalls #synthesis #SelfAssembly #plantcell

A cell wall-associated kinase phosphorylates NLR immune receptor to negatively regulate resistosome formation - Nature Plants The discovery of the resistosome was a milestone in plant immunity. Here, Zhong et al. identify a kinase that phosphorylates the NLR, and reveal how this kinase regulates resistosome assembly to fine-...

📜 A cell wall-associated kinase phosphorylates NLR immune receptor to negatively regulate resistosome formation

🧑‍🔬 Chenchen Zhong, Wenli Li, Yongliang Zhang

📔 @natureplants.bsky.social

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantCellWalls #PlantImmunity #PlantSignaling

Scientists Witness Living Plant Cells Generate Cellulose and Form Cell Walls For the First Time | Newswise In a groundbreaking study on the synthesis of cellulose – a major constituent of all plant cell walls – a team of Rutgers University-New Brunswick researchers has captured images of the microscopic pr...

Scientists Witness Living Plant Cells Generate Cellulose and Form Cell Walls For the First Time
www.newswise.com/articles/sci...
#cellulose #biofuel #plantcellwalls #synthesis #SelfAssembly #plantcell

New insights into bryophyte arabinogalactan-proteins from a hornwort and a moss model organism Two bryophyte models, the hornwort Anthoceros agrestis (Anthoceros) and the moss Physcomitrium patens (Physcomitrium), were analysed for presence of arabinogalactan-proteins (AGPs), as emergence of th...

📜 New insights into bryophyte arabinogalactan-proteins from a hornwort and a moss model organism

🧑‍🔬 Kim-Kristine Mueller, Lukas Pfeifer, Birgit Classen, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantEvolution #PlantCellWalls #PlantPhysiology

Nucleotide Sugar Transporters: Orchestrating Luminal Glycosylation in Plants | Annual Reviews Eukaryotic glycobiology revolves around nucleotide sugar transporters (NSTs), which are critical for glycan biosynthesis in the Golgi apparatus and endoplasmic reticulum. In plants, NSTs share similar...

📜 Nucleotide Sugar Transporters: Orchestrating Luminal Glycosylation in Plants

🧑‍🔬 @beritebert.bsky.social, Ariel Orellana

📔 Annual Review of Plant Biology

🔗 www.annualreviews.org/content/jour...

#️⃣ #PlantScience #PlantCellWalls #PlantTransporters #PlantPhysiology #PlantGlycomics #PlantProteomics

A Way to Interact with the World: Complex and Diverse Spatiotemporal Cell Wall Thickenings in Plant Roots | Annual Reviews Plant cells are defined by their walls, which, in addition to providing structural support and shape, are an integral component of the nonliving extracellular space called the apoplast. Cell wall thic...

📜 A Way to Interact with the World: Complex and Diverse Spatiotemporal Cell Wall Thickenings in Plant Roots

🧑‍🔬 Alex Cantó-Pastor, Concepcion Manzano, @bradylabs.bsky.social

📔 Annual Review of Plant Biology

🔗 www.annualreviews.org/content/jour...

#️⃣ #PlantScience #PlantCellWalls #PlantRoots

Atomic force microscopy imaging of plant cell walls A review of advancements in atomic force microscopy imaging of cell wall structure and analysis of the mechanical properties, specific binding sites, and s

📜 Atomic force microscopy imaging of plant cell walls

🧑‍🔬 Junbao Pu, Wenjun Zhang, et al.

📔 @plantphys.bsky.social

🔗 academic.oup.com/plphys/artic...

#️⃣ #PlantScience #PlantMicroscopy #PlantImaging #PlantAFM #AtomicForceMicroscopy #PlantCellWalls

A metagenomic ‘dark matter’ enzyme catalyses oxidative cellulose conversion - Nature A metalloenzyme capable of oxidatively cleaving cellulose, found in a microbial community specialized in lignocellulose degradation, could enable sustainable biofuel production.

📜 A metagenomic ‘dark matter’ enzyme catalyses oxidative cellulose conversion

🧑‍🔬 Clelton A. Santos, Mario T. Murakami, et al.

📔 @nature.com

🔗 www.nature.com/articles/s41...

#️⃣ #PlantScience #PlantCellWalls #PlantGenomics

Atomic force microscopy imaging of plant cell walls A review of advancements in atomic force microscopy imaging of cell wall structure and analysis of the mechanical properties, specific binding sites, and s

AFM imaging of plant cell walls doi.org/10.1093/plph...
@plantphys.bsky.social #Plantcellwalls #imaging #AFM