Funded through our Science Program, this technique expands the scientific toolkit in ways that can reshape what researchers are able to ask and answer. New research – #ExpansionMicroscopy enlarges cells up to 60x by embedding them in a water-absorbing gel www.quantamagazine.org/expansion-mi...
#Antibodies are often too bulky to reach every target in dense clusters, leading to incomplete labeling. #expansionmicroscopy solves this by physically separating proteins, which facilitates efficient labeling and reveals protein complexes.
Watch the full episode here: youtu.be/8cXV9J5NJQQ
Antibody-free NHS-ester labeling #protocol enabling dense, uniform visualization of cytoskeletal structures. Optimized for #expansionmicroscopy, it minimizes linkage error, improves coverage, and provides an internal #nanoscale ruler for accurate #expansion-factor correction.
bio-protocol.org/e5539
Why Is Studying Consciousness So Challenging?
🎥 Watch Episode #19 with Prof. Ed Boyden to explore how #optogenetics, #expansionmicroscopy, and #neurotechnology are transforming #neuroscience.
Watch the full episode on this link: youtu.be/fSVaFThb99w?...
Cephla is at #SfN2025 through Wednesday 11/19. Come to our booth at 3217 to check out our Squid+ spinning confocal and fluidics system + learn how we help accelerate developing/disseminating/adopting new methods and scale data production capacities!
#HCS #LiveCell #SpatialOmics #ExpansionMicroscopy
Helge @ewerslab.bsky.social knows not only how to organize conferences (#BiomembraneDays25) but also how septins organize. Exceptional imaging #ExpansionMicroscopy of cytokinesis
Who needs #clearing and #expansionmicroscopy?
Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.
#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX
Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.
#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX
Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.
#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX
Excited to give a talk next week at EMBO SRLM all about expansion microscopy! #expansionmicroscopy #ExM #EMBO
@science.org Expansion in situ genome sequencing links nuclear abnormalities to aberrant chromatin regulation | Science www.science.org/doi/10.1126/...
@jbuenrostro.bsky.social @eboyden3.bsky.social + al @broadinstitute.org @harvard.edu
#ExIGS #Chromatin #Lamin #Progeria #Aging #ExpansionMicroscopy
🔬What's that ? 👋 Go see our last publication: ERGIC in plant cell.
@yohannboutte.bsky.social @bic-bordeaux.bsky.social @cnrsbiologie.bsky.social @lbm-bordeaux.bsky.social @naturecellbiology.bsky.social
#tau-STED #ExpansionMicroscopy #CellBiology #PlantCell
Super-resolution microscopy unravels a dynamic tubular network that is stabilized by pre-existing Golgi and lipids to mature into new Golgi pre-cisternae, @bic-bordeaux.bsky.social, @cnrsbiologie.bsky.social, @cnrsaquitaine.bsky.social, #Lipids, #ExpansionMicroscopy, #PlantScience, #PlantCellBiology
However, three orders of magnitude didn’t seem so daunting to Ari, Yuanyou, and Nana, so they built a bacteria-optimized #ExpansionMicroscopy toolbox that is capable of tunable expansion of individual bacteria up to 1000-fold in volume! 5/
🌱🔬Excited to share my recent In Brief article published in @theplantcell.bsky.social @aspbofficial.bsky.social 🧪🌾
🔗 doi.org/10.1093/plce...
I enjoyed covering this #ThePlantCell paper and I look forward to seeing #PlantEx benefit the #PlantScience community.
#PlantBiology #ExpansionMicroscopy
Very happy to share our latest, a review on #ExpansionMicroscopy across organisms (including zebrafish, mice, Drosophila, and C. elegans) with @galhaspel.bsky.social and more :) #zebrafish #microscopy doi.org/10.1117/1.NP...
My '37th View of Mount Fuji,' is a January cover for @scotmicro's 2025 fundraising calendar!
Got it at SMS 50th Annual Symposium, where I gave a talk on #expansionmicroscopy :)
A 'mountain' formed by ependymal cell nuclei in the mouse brainstem.
#Microscopy #Neuroscience #Brain #FluorescenceFriday
This was such a great meeting. I would highly recommend joining this friendly network of cilia (AND drum-roll please!) centrosome enthusiats. The network is expanding #expansionmicroscopy lol!
📜 ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems
🧑🔬 Kevin L. Cox, Sarah A. Pardi, Kirk J. Czymmek, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ #PlantScience #PlantMicroscopy #ExpansionMicroscopy #PlantMethods
Ok, so #ExpansionMicroscopy has now reached the #PlantScience field 🔬🌾
Preprints from two labs out today on @biorxiv-plants.bsky.social
#1, Magali S. Grison et al.
bsky.app/profile/soms...
#2: Michelle Gallei, Sven Truckenbrodt, et al.
bsky.app/profile/soms...
#PlantMicroscopy #PlantCellBiology
📜 Super-resolution expansion microscopy in plant roots
🧑🔬 Michelle Gallei, Sven Truckenbrodt, Johann G. Danzl, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ (Feeds) #PlantScience #PlantMicroscopy #ExpansionMicroscopy #PlantCellBiology
"Cx43 plaques as potential keratinocyte-nerve fiber communication sites" in👤Skin
#SuperResolutionArrayTomography
#ExpansionMicroscopy
+Coculture hiPSC-sensory neuron + primary keratinocyte to model physical ensheathment & Ca2+ coupling
#eLife 2024
elifesciences.org/articles/77761
🤯 A conventional light microscope can now see a single synaptic vesicle! The shape of antibodies! Conformational changes of calmodulin!
With a new #ExpansionMicroscopy method👇 Great times for curious minds to be alive!
x.com/AliHShaib/stat…
Nice #ExpansionMicroscopy showing astrocyte contacts with synapses in brain sections www.cell.com/cell-reports/fulltext/S2...
