Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system This study developed two simple, efficient, and rapid genome editing tools termed Self-splicing Intron-Based Riboswitch-Cas9 (SIBR-Cas9) and SIBR2.0-Cas12a, for editing the genome of the industrially relevant microbial strain Cupriavidus necator H16.…

Online now: Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system

🚨Check out this amwesome new CRISPR tool for highly efficient genome engineering in C. necator led by @dellavallesimo.bsky.social and @eorsi.bsky.social 🔥🔥🔥

Microbes like Cupriavidus necator naturally grow on #renewable feedstocks ♻️🧫 (CO₂, formate, hydrogen), yet their potential remains untapped due to the lack of efficient genetic tools... 1/2

www.cell.com/trends/biote...

Our new #CRISPR toolkit for C. necator, built on SIBR-Cas technology, is now in press - an incredible collaboration with @eorsi.bsky.social, @copabio.bsky.social and many others🧪🧬⏳✂

Check out the thread below for details on genome editing + controlling gene expression by splitting a gene in two!