Figure 1 in Lubbers et al. (2025): " Phenotypic analysis of K. viridifaciens ΔdivIVA expressing divIVA variants. (A) Cartoon and surface representation of DivIVA of K. viridifaciens made using AF3. Structured domains are represented in blue, and unstructured domains are depicted in light brown. The PAE plot is shown on the right. Low confidence values are yellow, high values are blue. pLDDT values—per-residue measures of local confidence—can be found in electronic supplementary material, figure S1. (B) L-form strains expressing different divIVA alleles were tested for growth as L-forms on LPMA medium (left two images) and for the ability to revert to walled growth on non-osmoprotective plates (right two images). Scale bar = 2 mm. (C) Schematic overviews showing the architecture of the DivIVA variants from panel B, relative to the wild-type protein (top), which contains four domains: an N-terminal domain (M1-N65), which we have subdivided into a membrane-binding structure (N) (M1-D23) (light red line) and a coiled-coil region (CC1) (E24-N65) (light blue box), an intercoil region (IR) (M66-G224) (yellow line), a larger coiled-coil domain (CC2) (D225-P345) (dark blue box), and a C-terminal domain (C) (P346-N445) (dark red line). (D) Quantification of the average colony area for strains expressing DivIVA variants (n = 30). (E) Quantification of colony solidity for strains expressing DivIVA variants (n = 30). Solidity was calculated by dividing the colony area by the convex area. (F) The number of reverted colonies per strain per reversion plate (n = 3)."
New publication: Essential and dispensable domains of #DivIVA for walled growth in filamentous #Actinomycetota, by @gillesvanwezel.bsky.social and others. #hyphalgrowth
doi.org/10.1098/rsob...
