Together, our data shows that increased transcriptional levels of #AluJB are sufficient to drive changes in many cellular processes related to the #hallmarks of #aging - thus suggesting that #TE derepression may exert causal effects on the #aging process rather than be a mere bystander. 8/9
This led us to #functionally test whether these processes were directly impacted in IMR90 fibroblasts overexpressing #AluJB. Consistent with omic predictions, we observed decreased #respiratory rates, #S-phase accumulation and changes in #aggresome load! 7/9
Thus, we decided to overexpress #AluJB in primary IMR90 fibroblasts, and perform multi-omic profiling of its impact on the #transcriptome, #proteome and #secretome. We were surprised to see large scale multi-omic remodeling in response to #AluJB. 4/9
Intriguingly, higher endogenous #AluJB transcriptional levels were associated with enrichments of pathways related to #aging #hallmarks, including #proteostasis, cell cycle and #ECM remodeling. 3/9
