What are your tips and tricks for speaking about fish breeding to a young audience? 🐟🥚 #AskZebrafish
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Image of the bat signal but instead of a bat it's a zebrafish. Also, it's dark and rainy.
Another #AskZebrafish from Slack: Does anyone that uses #zebrafish have a favorite photoconvertible protein? Ideally one that is well-suited for membrane labeling? 🤔
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Image of the bat signal but instead of a bat it's a zebrafish. Also, it's dark and rainy.
Query from Slack: Looking for wls (wntless) and/or wnt5a/wnt5b mutant #zebrafish lines in Europe? If you have these or know any leads, please reach out 🙏 #AskZebrafish
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Query from Slack: Does anyone in the UK have a red colour pu.1 (spi1b) reporter line for all myeloid cells? Any help and/or leads appreciated! 🔎 #AskZebrafish
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Image of the bat signal. But instead of a bat, it's a zebrafish. Also, it's rainy and dark.
Query from UK Fish Locator: A lab in the UK is looking for the grb10a^sa36837 mutant (zfin.org/ZDB-ALT-1610...). Any leads appreciated! #AskZebrafish
Want to learn more about the UK Fish Locator? See: www.crick.ac.uk/research/pla...
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Image of the bat signal but instead of a bat, it's a zebrafish. Also, it's raining.
Query from Slack: Looking for wt1a and wt1b reporter transgenics swimming in the USA and a lab willing to share them? Any leads are thoroughly appreciated! 🔎 #AskZebrafish
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A little late now for the Americans but we are curious 🧐 How does your facility approach Daylight Savings Time? Small gradual changes or a big one-off? #AskZebrafish
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Do you remember your first #zebrafish embryo sighting? #AskZebrafish
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Query from Slack: Looking for anyone with a broken/unused Narishige PC-10 capillary puller 🔍 A lab is looking for a spare part (a screw!), but it is now fully discontinued. Any leads appreciated! #AskZebrafish
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Query from Slack: Looking for the Tg(ubb:SEC-Hsa.ANXA5-tdTomato)^xt24 apoptosis transgenic line from the Tobin Lab swimming in the 🇺🇸. If your lab has it (and you are willing to share 😊), please reach out! 🙏 #AskZebrafish
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For those willing to help members of the #zebrafish community, we have a few unanswered questions/queries from the last month that we've compiled in this thread. Any insight, feedback or recommendations are thoroughly appreciated! Please reach out 🙏 #YouRock #AskZebrafish 1/8
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🔍 #AskZebrafish: There's rumors that labs are having trouble finding good quality Alcian Blue. If you do know of some good stuff, what brand/lot of Alcian blue does your lab use? Alternatively, anything to avoid would be appreciated 🙏
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🔎 Hoping to find it in Europe 🌍 Any leads appreciated! #AskZebrafish
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🤔 #AskZebrafish: Has anybody tried using the anti-MBP antibody (dshb.biology.uiowa.edu/Anti-MBP-BCN...) from @dshb-antibodies.bsky.social? A lab wants to use it for recognizing glia in 2-5 dpf #zebrafish. Alternatively, if anyone has any recommendations that would work instead, please let us know 🙏
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🔍 Query from Slack: Would anyone in Europe have the sqet33 line from the Korzh Lab swimming about? From this paper: journals.plos.org/plosone/arti... #AskZebrafish
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Headscratcher from Slack: "Has anyone implemented fin-clip qPCR to verify casper background (mitfa/mpv17 expression) — or has any advice? We believe we have a contamination in a line and we want to get rid of any 'casper'" 🤔 #AskZebrafish
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Query from Slack: Does anyone have the line or the plasmids to make the heat shock lines for isl1a and isl2a? These ones: zfin.org/ZDB-TGCONSTR... and zfin.org/ZDB-TGCONSTR... 🔎 Any leads appreciated! #AskZebrafish
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Any other recommendations from the community? 🤔 #AskZebrafish
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Query from Slack: Does anyone anywhere still have the Mione lab's Tg(UAS:eGFP-H-RAS_GV12)io6 swimming about? 🤔 #AskZebrafish
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Another query from Slack: "Does anyone here have a favorite destabilized GFP (or similar) fluorophore? Ideally tested [in zebrafish] with a decent transgene - any pointers appreciated!" #AskZebrafish
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Query from Slack: Looking for someone to share a floxed KRAS transgenic line (preferably in the UK) or a plasmid that has already been tested (anywhere). Any help and/or leads would be greatly appreciated! #AskZebrafish
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Another query from Slack: Is there anyone with experience isolating and culturing zebrafish pigment cells (melanophores/iridophores/xanthophores)? Any input or advice on how to make it work would be useful! #AskZebrafish
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Query from Slack: Has anyone tried to express HaloTag7 in zebrafish? If yes, could you please share the plasmid(s) with a fellow fish lab? 🙏 #AskZebrafish
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