Screening of expanded GRACE mutant collection at six temperatures reveals distinct functional enrichment in genes clustered by fitness profile. Top left: Schematic of GRACE mutant construction. Bottom left: Summary of GRACE mutant collection expansion based on manual curation of biological process gene ontology annotation on Candida Genome Database (CGD). Pie chart with four concentric rings visualizes the relative proportion of genes from various versions of the GRACE collection with biological process descriptions completely unannotated or annotated based on: experimentally characterization, S. cerevisiae ortholog, S. pombe ortholog, orthologs in other fungal species, conserved protein domains, or unannotated; see Materials and methods for full details. Right: Heatmap of genes ordered by hierarchical clustering of essentiality scores across screening temperatures. A score of 4 (red) represents no growth and 0 (yellow) represents growth comparable to wild type.
#Candida albicans is a leading opportunistic fungal #pathogen of humans. Ci Fu, Leah Cowen &co expand the GRACE #Calbicans #FunctionalGenomics resource, identifying genes important for temperature-dependent fitness & highlighting its power to reveal vulnerabilities @plosbiology.org 🧪 plos.io/3Wft0Xg
