❓Tech Q&A | Use 10 μM for 5-30 min (commonly 20 min) at 37°C.
🧪Dilute 1:1000 in serum-free medium, cover #cells, wash 3× with serum-free medium.
🔃Order matters: short (≤2 h) load probe➡️stimulate; long (≥6 h) stimulate➡️load #probe.
✅Include a positive control (e.g. #CCCP).
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