schematic representation of various proteins in mitochondria of a microbial cell
New #ISEPpapers! The mitochondrial proteome of #diplonemids: from conventional pathways to eccentric RNA editing and transcript processing link.springer.com/article/10.1...
#Protists #Microbes #Mitochondria #Proteomics #Evolution
Endogenous tagging of novel proteins confirms supervised cluster predictions. Left: Tagged proteins highlighted (black) among relevant predicted clusters, resolved on neighbor-averaged imputed t-SNE. Right: Individual cell lines were generated via endogenous tagging and imaged through fluorescence microscopy for comparison with the compartment-relevant protein was predicted to. Merged microscopy images showing protein signal (green) merged with nuclear and mitochondrial DNA (blue). All imaged cells are oriented with their apical regions facing right and posterior facing left, cell membrane outlines are traced for all images except for L, showing only trace of the papilla, which lacks signal. Scale bar represents 5 μm.
#Diplonemids are among the most diverse & abundant #protists in the deep #ocean. This study localises >5,000 proteins of #Paradiplonema papillatum, revealing #organelle compartmentalisation, including a membrane coated with carbohydrate-degrading enzymes @plosbiology.org 🧪 plos.io/3XvmpIO
Endogenous tagging of novel proteins confirms supervised cluster predictions. Left: Tagged proteins highlighted (black) among relevant predicted clusters, resolved on neighbor-averaged imputed t-SNE. Right: Individual cell lines were generated via endogenous tagging and imaged through fluorescence microscopy for comparison with the compartment-relevant protein was predicted to. Merged microscopy images showing protein signal (green) merged with nuclear and mitochondrial DNA (blue). All imaged cells are oriented with their apical regions facing right and posterior facing left, cell membrane outlines are traced for all images except for L, showing only trace of the papilla, which lacks signal. Scale bar represents 5 μm.
#Diplonemids are among the most diverse & abundant #protists in the deep #ocean. This study localises >5,000 proteins of #Paradiplonema papillatum, revealing #organelle compartmentalisation, including a membrane coated with carbohydrate-degrading enzymes @plosbiology.org 🧪 plos.io/3XvmpIO
Endogenous tagging of novel proteins confirms supervised cluster predictions. Left: Tagged proteins highlighted (black) among relevant predicted clusters, resolved on neighbor-averaged imputed t-SNE. Right: Individual cell lines were generated via endogenous tagging and imaged through fluorescence microscopy for comparison with the compartment-relevant protein was predicted to. Merged microscopy images showing protein signal (green) merged with nuclear and mitochondrial DNA (blue). All imaged cells are oriented with their apical regions facing right and posterior facing left, cell membrane outlines are traced for all images except for L, showing only trace of the papilla, which lacks signal. Scale bar represents 5 μm.
#Diplonemids are among the most diverse & abundant #protists in the deep #ocean. This study localises >5,000 proteins of #Paradiplonema papillatum, revealing #organelle compartmentalisation, including a membrane coated with carbohydrate-degrading enzymes @plosbiology.org 🧪 plos.io/3XvmpIO
