This is a hematoxylin and eosin stained section of kidney from a bearded dragon. Encephalitozoan pogonae, a microsporidian, expands they cytoplasm of many cells. Nuclei of cells are dark purple and the parenchyma is mostly pink (eosinophilic). The microsporidians are elongate. They have a polar tube and posterior vacuole that create some differential staining of the organism.

This is an acid-fast stained section of kidney from a bearded dragon. Encephalitozoan pogonae, a microsporidian, expands they cytoplasm of many cells and these organisms are stained a bright fuchsia color. The counterstain turns the background tissue light blue.

This is a gram stained section of kidney from a bearded dragon. Encephalitozoan pogonae, a microsporidian, expands they cytoplasm of many cells. Nuclei of cells are verydark purple. The cells and fibrous connective tissue in the backgrounds are pink . The microsporidians are elongate. Although many are aggregated in host cells, some are scattered in the tissue.

Check out the massive numbers of #microsporidia in these sections! How many times have you had to search for #Encephalitozoon cuniculi? This is E. pogonae in #kidney of a bearded dragon. The lizards know how to grow Encephalitozoon.

#PathSky #VetPath #Veterinary #Parasitology #Histopathology