Immunofluorescence Staining of E17.5 Ai6 x Eomes-Cre Placentae for Mesenchymal Cells. EomesPOS cells and daughters (ZsGreen; green), all counterstained with NucBlue (DAPI; blue). (A) Staining of endothelial cells in the chorion and lower labyrinth layer with an anti-CD31 antibody (red; 400x). Ai6NEG (above) and Ai6POS (below). (B) Staining of placental pericytes in the labyrinth layer with an anti-αSMA antibody (red; 400x). Ai6NEG (above) and Ai6POS (below). White arrowheads show colocalization of ZsGreen with antibody staining.

#DBfeature

Lineage tracing of Eomes-positive mouse trophoblast cells confirms their capacity to contribute to both placental layers in vivo even after E7.5

-Avery McGinnis, Megan Cull, Nichole Peterson, Matthew Tang, Bryony Natale @bnatale.bsky.social, David Natale

#Extraembryonic

Diagram of early implantation steps. Early blastocyst at E3.5 receives a "Signal triggering attachment". The blastocyst is then showed at E4.0 as it attaches to the uterine epithelium via glandular connections. At E4.5 mTORC1 is activated and starts a "Signal triggering protrusive activity". At E4.8 the blastocyst is shown at the onset of invasion of the uterine epithelium.

#DBFeature 🥚

Amino acids activate mTORC1 at the early blastocyst stage to regulate trophoblast motility, controlling embryo implantation and the ability to enter diapause in both mouse and human blastocysts.

By Ann Sutherland

tinyurl.com/2kt9a6up

#SpecialIssue on #ExtraEmbryonic tissues

Diagram of a pregnant mouse and an embryo in a falcon tube, both pointing towards an enlarged schematic of an embryo in its extra embryonic tissues. A green arrow points towards the embryo from the mouse with the caption "Reichert’s membrane = essential". A red arrow points from the falcon tube to the embryo with the caption "Reichert’s membrane = detrimental". Reichert’s membrane is highlighted in blue in the embryo depiction.

#DBFeature 🥚

Reichert’s membrane cushions the mammalian embryo from uterine contractions, but paradoxically hinders growth in embryo culture.

By Andrew Copp

tinyurl.com/49rrr7wj

#SpecialIssue on #ExtraEmbryonic tissues

Extraembryonic gut endoderm cells undergo programmed cell death during development - Nature Cell Biology Batki, Hetzel et al. report a lineage-tracing strategy to track extraembryonic gut endoderm cells over development. They find that these cells are eventually eliminated in a p53-dependent manner and n...

#NCB2024
In June, Meissner & co reported a lineage-tracing strategy to track #extraembryonic gut #endoderm cells over development; showed these cells are eventually eliminated in a p53-dependent manner and neighboring embryonic cells clear their remnants.
www.nature.com/articles/s41...

Origin, fate and function of extraembryonic tissues during mammalian development - Nature Reviews Molecular Cell Biology During mammalian embryogenesis, extraembryonic tissues are required for the patterning and morphogenesis of the embryo. This Review discusses how signalling networks and epigenetic modifications regul...

⏰New @natrevmcb.bsky.social Review!

@shifaant.bsky.social, @cwhanna.bsky.social & @srinivas-lab.bsky.social explore how #extraembryonic tissues contribute to mammalian development.

✨https://go.nature.com/3BeQIfm✨