On Friday's, we fluoresce! For this #FlourescenceFriday we have an image from Derrick Graham of a zebrafish gut that was infected with Vibrio cholerae, labelling dead cells in red and cell nuclei in white, showing widespread cell death in infected guts.
Image of a larval zebrafish gut. In this image filamentous branches of actin are visible as simple cyan lines. The overall effect reveals the structure of the gut as a relatively simple tube that runs from front to back. However, close examination also shows that the tube contains multiple folds that greatly expand the surface area of the gut, increasing the absorptive capacity.
Here's a cute #FlourescenceFriday offering. One of the last images from Canada: A larval gut stained by Alisha Duggal with phalloidin to visualise F-actin. I love how effectively this captures the architecture of a developing gut.
Images by Reegan Willms. The upper panel shows a larval zebrafish with bright white cells reporting active immune signalling throughout the animal. In the lower panel, the image zooms in on the gut demonstrating a strong host response, much of which is to the microbial flora that typically establishes residence in the gut at this stage.
A little #FlourescenceFriday Fish Fun from the vault: Reegan capture this image of 5dpf larvae that express GFP under control of the NF-kB promoter. It's always fun to see how NF-kB is firing away doing it's thing in the gut, even in the absence of any kind of stress or infection.
Happy #FlourescenceFriday! Our #CellLineOfTheWeek is SON, a cell line and plasmid that illuminates ✨ nuclear speckles ✨ a real science word for nuclear proteins involved in gene expression.
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
Confocal micrograph of a nucleus stained in orange and the primary cilium in green. The color combination makes it look like a pumpkin.
It's Halloween 🎃 #FlourescenceFriday! Here is a microscopic pumpkin generated by showing a cell nucleus in 🟠 with the primary cilium "stem" in 🟢! I hope everyone has a spooky 👻 day! 🔬 by @stjuderesearch.bsky.social grad student Christina Wang. 🧪
A mostly green tinted picture of a histological brain section. The upper left part shows a faint egg-shaped structure, close to which a red stripe starts, going towards the right side. A small scale bar at the top gives the size range (500 microns).
It's #FlourescenceFriday ! This is the optic tectum of a ring-billed gull brain. The red areas are made up of very young neurons, while the green are mature. The egg-shaped structure in the top left part is the nucleus pretectalis principalis, which plays a role in processing visual information.
Happy #FlourescenceFriday and #OpenScienceWeek! Our #CellLineOfTheWeek is Myosin Light Chain 2v. 💪🧫
Our cell lines & plasmids are available for just the cost of shipping to support scientific discovery.
📦 Hosted by @coriellinstitute.bsky.social & @addgene.bsky.social
Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right).
Stain: @spirochrome.com SiR-Tubulin
Happy #FlourescenceFriday!
With everything that's going on, I needed this cool data 1 day before #flourescencefriday. It's my first time with #GCaMP imaging (green), and you can clearly see Aβ42 induced hyperexcitability at the same time that we also see BBB leakage (not shown)! #zebrafishrock
"Zebrafish head"
In this captivating image, we can see the labeling of blood vessels in the head of a #zebrafish. Taken by Marko Pende on the #mesoSPIM in December 2022.
#flourescencefriday 🐟🎸 🧪🤝 #microscopy #fluorescentproteins #lightsheet
Since we missed #FlourescenceFriday, here is our first Bluesky #MicroscopyMonday post. Thanks to our village to help us troubleshoot! Next up.... the rig.
I love this. Maybe we can get #MacrophageMonday to stick and then we’d have #TcellThursday along with #FlourescenceFriday and it would be beautiful immunology all week long.
A little late entry for #flourescencefriday, but here's the vasculature of the adult murine leg.
