HiBiT-based NanoBRET® assays offer real-time insight into GPCR–ligand binding in live cells - no radioligands, no wash steps.

A great tool for receptor pharmacology.

Read the full blog at https://tinyurl.com/HiBiTBasedNanoBRETBlog

#GPCRResearch #NanoBRET

🎤 Meet Dr. McGarvey — #ENABLE2025 Career Talks speaker!

🧬 Senior Scientist @ Nxera
🧠 PhD in Neuroscience (UoBristol)
🔬 Postdoc @ University of Pittsburgh on GPCR signaling
🌱 STEM mentor with In2Research & STEM Ambassadors

#FEBSIUBMB #CareerTalks #STEMcareers #LifeSciences #GPCRresearch #PhDlife

An engineered trafficking biosensor reveals a role for DNAJC13 in DOR downregulation | Nature Chemical Biology Trafficking of G protein-coupled receptors (GPCRs) through the endosomal–lysosomal pathway is critical to homeostatic regulation of GPCRs following activation with agonist. Identifying the genes involved in GPCR trafficking is challenging due to the complexity of sorting operations and the large number of cellular proteins involved in the process. Here, we developed a high-sensitivity biosensor for GPCR expression and agonist-induced trafficking to the lysosome by leveraging the ability of the engineered peroxidase APEX2 to activate the fluorogenic substrate Amplex UltraRed (AUR). We used the GPCR–APEX2/AUR assay to perform a genome-wide CRISPR interference screen focused on identifying genes regulating expression and trafficking of the δ-opioid receptor (DOR). We identified 492 genes consisting of both known and new regulators of DOR function. We demonstrate that one new regulator, DNAJC13, controls trafficking of multiple GPCRs, including DOR, through the endosomal–lysosomal pathway

A novel biosensor reveals DNAJC13's role in GPCR downregulation, highlighting its function in endosomal-lysosomal trafficking. #GPCRresearch PMID:39223388, Nat Chem Biol 2025, @nchembio https://doi.org/10.1038/s41589-024-01705-2 #Medsky 🧪