Figure 1 from Dao et al. 2025, consisting of four figures showing that GPS2 transient expression reveals genotype and tissue differences in FRET ratio after GA treatment. (Panel a) Confocal section showing transient expression of GPS2 after biolistic bombardment, which yields characteristic nuclear signal of CFP (left), CFP to AFP FRET ex/em (middle), and AFP ex/em (right). (panel b) Fluorescence quantification based on mask multiplication between binarized channels allows identification of GPS2-expressing regions of interests (ROIs) and calculation of FRET/CFP emission ratio. (Panel c) Box plots showing leaf tissues transiently expressing GPS2 in maize, barley, sorghum, and wheat display a FRET/CFP emission ratio response when treated with increasing levels of GA. Note that maize d1, GA biosynthesis mutant, displays a decrease in FRET ratio. (Panel d) Box plots showing floral tissues transiently expressing GFP2 in maize and barley responding to GA, with maize only responding after high GA treatments (10 uM and 100 uM).
Hormonal changes...🌽
Dao et al have quantified #GibberellicAcid and #Auxin responses across multiple #maize #barley #sorghum & #wheat tissues/genotypes, identifying common cereal responsive genes & reliable biosensors for use in future studies
doi.org/10.1093/pcp/...
#PlantScience
#Phytohormones