Very dynamic structures in #live #cell going through the division: lysosomes (yellow) and tubulin (magenta). #FluorescentFriday fun! #GOLIVE #gomulticolor

Hmm... An intriguing question: do we really need fluorogenic probes? See SIM no-wash images with tubulin probes, but only one is fluorogenic. Do you see a difference? #tubulinthursday #fluorescentfriday #goLive #gomulticolor

Checking STED system after Covid-19 vacations. Two-colour 3D iso-STED works OK. Enjoy nice image tubulin (green) and mitochondria (red) in living cells. Sub-diffraction in all directions ! Thanks to @Abberior and @svi_huygens . #GOLIVE #gomulticolor

Excellent performance of 6-610CP-JAS in living human fibroblasts. See multiple layers of actin inside the cell. Read our article published in @OrgBiomolChem : pubs.rsc.org/en/content/articlelandin... #Golive #gomulticolor #NoVerapamil

As we have promised, the paper about the super-permeable nanoscopy compatible fluorescent probes is out on @biorxivpreprint ! Just a minor change in the targeting moiety attachment point and you get the best probes! biorxiv.org/cgi/content/short/2020.0... #GoLive #gomulticolor

Can it be something better than SiR-actin? Yes, it can! This is 6-610CP-JAS (yellow). If you have trouble with staining cells due to efflux read this, fresh on @biorxivpreprint : www.biorxiv.org/content/10.1101/2020.02.... #GoLive #Notransfection #gomulticolor

For those who search properties of cell permeable dyes, here's a table with summary of some often used rhodamine dyes. We've selected orange/red spectral region because of their excellent biocompatibility. Enjoy! #golive #gomulticolor #livingcell

Living cells super-resolution imaging does not have to be expensive! See movie of living fibroblasts stained with our newest fluorogenic probes for tubulin (green) and DNA (magenta) taken on STEDYCON from @AI_ontour . Interested, contact us. #gomulticolor #GoLive #notransfection

A set of green-emitting rhodamine dyes for staining of living cells and compatible with super-resolution microscopy. #golive #gomulticolor #notransfection
Nice work from our department and Abberior chemists: onlinelibrary.wiley.com/doi/abs/10.1002/cbic.201...

Hmm... large field imaging is always attractive! It gives excellent statistics and produces more reliable results. Enjoy bird's-eye view on the living cells stained with actin and tubulin probes. #golive #gomulticolor #notransfection

Sometimes labelled cell looks beautiful and excellent performance of @AI_ontour STED microscope helps to show it! Enjoy perfect image of microtubules (yellow) and mitochondria (magenta) in living cells. #golive #gomulticolor #notransfection

Testing our new automated microscope for living cell imaging. Enjoy movie of living Hela cells stained with our newest probes for tubulin and DNA. Works well without washing! #GoLive #GoMulticolor

Challenge of 2019: four-color imaging without SiR probes! GeR-tubulin (magenta), 580CP-actin (cyan), CX-mitochondria (orange), BODIPY FL-membrane and endosomes (yellow). #golive #gomulticolor
Interested, contact us and see papers:
https://t.co/HN9dfC3r6E
https://t.co/ydBqZKY1Aj

The 3rd prize of SVI contest goes to us! #gomulticolor #golive
https://svi.nl/winnersimagecontest2018

Christmas are coming - new dyes are arriving! New dyes are cell-permeable and do not give color shift while imaging. #gomulticolor #goLive
Just published in @J_A_C_S
Triarylmethane Fluorophores Resistant to Oxidative Photobluing pubs.acs.org/doi/10.1021/jacs.8b11036

Two-color living-cell STED: highly efficient labelling with SiR-CTX (tubulin, magenta) and 5-580CP-Hoechst (DNA, yellow). #golive #gomulticolor
Interested, contact us!
Deconvolved with @svi_huygens
All published in @ChemicalScience
https://t.co/Ugky5q97uQ
https://t.co/ydBqZKY1Aj

Christmas is close and cells get ready: actin, talin, tubulin and mitochondria are blinking all the colors in living cells. #GoLive #gomulticolor #notransfection #Christmas

Large stokes shift (LSS) dyes challenge: see two colors with single excitation light source by eye and record the image with smartphone. GFP-talin + SiX-actin in living cells. Interested, contact us! #gomulticolor #GoLive #notransfection #smartphone
https://pubs.acs.org/doi/abs/10.1021/jacs.7b06412

Another challenge: four color living cell imaging without SiR probes. Blue - actin, green - tubulin, yellow - endosomes, magenta - mitochondria. Interested, contact us! #gomulticolor #notransfection #GoLive
https://t.co/IvJ9XfzLcZ
https://t.co/ydBqZKY1Aj

Another day, another three-color living cell video: mitochondria, lysosome and microtubule interplay. The key players are cell-permeant large stokes shift dyes: SiX, GeX and CX. Interested, contact us! #gomulticolor #livingcells #notransfection
https://pubs.acs.org/doi/abs/10.1021/jacs.7b06412

We challenged us: three color living cell imaging without SiR probes and it worked. Interested, contact us! #gomulticolor