The contacts of the catalytic domain with DNA, on the other hand, are responsible for maintaining #H3K36ac selectivity.
Mutating a key interacting loop helps release the enzyme from the nucleosome and become much more active on #H3K18ac substrates 🤯.
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By comparing structures trapped at each substrate position we could rationalize its activity and intrinsic selectivity 🔍.
#SIRT7 binding is optimal for targeting #H3K36ac, and it can only access #H3K18ac through partial disengagement from the nucleosome and DNA bending.
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