New in JB: Treen, Ojha et al. update us on the latest research (and challenges) of studying Mycobacterium abscessus, an important pathogen and growing in CF. Its open pan-genome represents a significant challenge.
journals.asm.org/doi/10.1128/...
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New in JB: A team of researchers explored metabolic patterns associated with commensal vs. extraintestinal pathogenic E. coli. They identified clone-specific metabolic processes that may support extraintestinal survival or gut colonization.
journals.asm.org/doi/10.1128/...
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New in JB: Guo, Zhang et al. review recent advances in synthetic biology that allow for the use of methylotrophic bacteria as "cell factories" as a path to sustainable biomanufacturing.
journals.asm.org/doi/10.1128/...
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Moore, Gitai and colleagues report "CauloKO:an ordered transposon mutant library in Caulobacter crescentus", and use my favorite assay - a crystal violet screen for biofilm mutants, validating the libray and identifying new mutants.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
New in JB: Slamti and Lereclus review the role of Bacillus thuringiensis, the toxins it produces and its relationship to insects in a wide ranging and interesting review.
journals.asm.org/doi/10.1128/...
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New in JB: Pritchet, Damron & Barbier explore the role of PilY1, one of may favorite proteins (which is involved in surface sensing) in both signaling and virulence.
journals.asm.org/doi/10.1128/...
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The newest version of JB is out. A remembrance of Mike Cashel, a commentary on surface sensing, multiple minireviews and articles. Check it out!
journals.asm.org/toc/jb/208/3
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Mutation of Dma2 causes morphological changes in Bt cells. Representative slices through cryo-electron tomograms of OMVs from (A) Bt WT and(B) Δdma2. Slices through cryo-electron tomograms of rod-shaped and coccoid cells, and their corresponding ratios from (C-E) Bt WT and (F-H) Δdma2. Thisshows that the mutation of dma2 increases the number of coccoid cells present in Bt.
Dual membrane-spanning anti-sigma factor 1 (Dma1) increases OMV production in B. thetaiotaomicron. Meanwhile, Dma2 modulates vesiculation and the expression of select polysaccharide utilization loci targeting host-associated glycans. #JBacteriology: asm.social/2R4
New in JB: Chourashi, Oglesby et al. show that PrrF sRNAs and PqsA and contribute to biofilm formation at infection-relevant temperatures, linking body temperature, iron homeostasis & biofilm physiology in P. aeruginosa.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
New in JB: Kang, Hwang et al. identify a two-component system for sensing and neutralizing toxic environmental ammonia. This system is likely imprtant in environments where amino acids are catabolized, leading to ammonia production.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Methodology for physical construction of the C. crescentus CB15 CauloKO library. C. crescentus CB15 cells were mated with pMiniHimar1 containing E. coli WM3604, and resulting colonies were handpicked in 96-well plates. Plates were pooled into 54 sequencing pots that were barcoded and PCR amplified before sequencing. The coordinates for sequenced mutants were then determined using the CauloKO GUI generated in this study.
Caulobacter crescentus is a gram-negative bacterium that has served as a model for understanding bacterial physiology with a myriad of genetic tools. In #JBacteriology: CauloKO—the first ordered, transposon library in C. crescentus. Learn more: asm.social/2Qw
New in JB: Cutts, Kim et al. report the development of a CRISPRi system using an endogenous, catalytically inactivated Cas9 (dCas9) in the COH1 strain of Streptococcus agalactiae. They use the system to knock down multiple virulence genes.
journals.asm.org/doi/10.1128/...
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New In JB: Phale, Kasarlawar et al. review the unusual traits of Pseudomonas bharatica CSV86T, an organism that they offer as useful for both bioremediation and up-cycling aromatic compounds.
journals.asm.org/doi/full/10....
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New in JB: Downing, Nayak et al. detail differences in acetoclastic methanogenesis between Methanosarcina spp. and Methanothrix spp. leveraging a combination of comparative genomics and molecular genetics.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
New in JB: Hartzler, Metskas et al. review recent information about α-carboxysome structure, mechanism, and assembly through the lens of cryo EM and tomography. A really exciting topic - one of my favorite areas to include in my grad course!
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Our newest paper by Sarvesh Surve, a PhD student in the lab, explores the culturome of the regions of the gut for persons with CF. We see clear differences from healthy controls, esp. in the mucosal samples.
journals.asm.org/doi/10.1128/...
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New in JB: Smith, Unnikrishnan et al. connect daptomycin sensitivity to the staphylococcal type VII secretion system protein EsxC via changes in the membrane, linking pathogenesis to Abx sensitivity.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Excited about a recent paper from Bassam el Hafi, a recently graduated PhD student, & Fabrice Jean Pierre leveraging 4,200 respiratory microbiomes from CF (pre- and post-ETI), and testing one of the hypotheses that arose from this analysis.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
JB Editor's Pick: Westlake, Criss et al. show that the neuroendocrine hormone norepinephrine depresses the Fur operon of Neisseria gonorrhoeae, perhaps via direct demetalation of Fur, resulting in increased survival of this microbe.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
New in JB: Adams & El-Halfawy review how various chemicals produced at the site of infections, including those made by the host & bacteria, impact Abx responsiveness. Learning about such mechanisms could help develop new Abx strategies.
journals.asm.org/doi/10.1128/...
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JB Editor's Pick: Westerman, Elfenbein et al. explore the mechanisms of sulfite stress resistance via their ability to detoxify these compounds, a key pathway for Salmonella in the host and when exposed as a food additive.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
JB Editor's Choice: As part of our History of Microbial Model Systems series, Snow, Hatzios & Salama discuss the first bacteria designated a class I carcinogen, Helicobacter pylori.
journals.asm.org/doi/10.1128/...
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JB Editor's Pick: Iglesias, Mongiardini et al. show that loss of the Tad pili of B. diazoefficiens results in enhanced biofilm formation - they propose a regulatory role, perhaps via cdG, for Tad impacting biofilms in this bug.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
The newest issue of JB is out! Topics include carboxysomes, Helicobacter & cancer, Tad pili, sulfite stress, norepinephrine & Fur, & more.
journals.asm.org/toc/jb/208/2
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I had the fun opportunity to write a Commentary for a JB paper by Bhattarai, Harshey et al. describing their Texas Two-Step model of cdG-mediated motility control.
Commentary: pubmed.ncbi.nlm.nih.gov/41705806/
Paper: journals.asm.org/doi/10.1128/...
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New in JB: George, Cahoon et al. document the role of PrsA1/2, parvulin peptidyl-prolyl isomerases, as post-translocation secretion chaperones, and demonstrate their impacts of cell physiology in L. monocytogenes, Streptococcus & S. aureus.
journals.asm.org/doi/10.1128/...
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New in JB: Askar, Chu et al. review the function of the aminopeptidase M17, a metallopeptidases, in bacteria and its potential role as a drug target. This review also nicely covers the history of research of this group of enzymes.
journals.asm.org/doi/10.1128/...
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New in JB: Christe, Windbergs et al. use Ramen spectroscopy to study membrane vesicles across the growth phases, revealing changes that could have important impacts on function.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
New in JB: Weiss, Tamayo et al. describe a very cool microbial interaction wherein Enterococcus faecalis, an opportunist found in the inflamed gut, impacts phase-variation noted by chaining & rough colony morphology of C. diff.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
