BioMassSpec

@realbiomassspec.bsky.social

2 weeks ago

Metabolic disparities of potentially toxic components derived from Psoraleae Fructus and Euodiae Fructus within the Sishen wan influenced by varying compatibilities and physiological conditions in an ... Sishen wan (SSW) is utilized in the treatment of irritable bowel syndrome (IBS). This study aimed to compare gut microbiota-mediated metabolic profile…

Metabolic disparities of potentially toxic components derived from Psoraleae Fructus and Euodiae Fructus within the Sishen wan influenced by varying compatibilities and physiological conditions in an in vitro gut microbiota model #JPBA #MassSpec www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 weeks ago

A universal immunocapture LC−MS/MS method for humanized IgG1−based drugs in cynomolgus monkeys The application of immunocapture LC−MS/MS in drug testing has expanded considerably over the past decade, offering significant advantages in analysis,…

A universal immunocapture LC−MS/MS method for humanized IgG1−based drugs in cynomolgus monkeys #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 weeks ago

Detection and quantification of ezetimibe and its major glucuronide in patients with hepatic impairment via liquid chromatography-tandem mass spectrometry Following administration of ezetimibe (EZE), levels of the glucuronidated metabolite ezetimibe-glucuronide (EZEG) are altered in patients with hepatic…

Detection and quantification of ezetimibe and its major glucuronide in patients with hepatic impairment via liquid chromatography-tandem mass spectrometry #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

3 weeks ago

Unraveling the Heterogeneity of Hyperuricemia Using Untargeted Metabolomics and Statistical Modeling Hyperuricemia is the second most prevalent metabolic disorder globally and exhibits pronounced heterogeneity in clinical practice. Only about 10% of i…

Unraveling the Heterogeneity of Hyperuricemia Using Untargeted Metabolomics and Statistical Modeling #JPBA #MassSpec www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

3 weeks ago

Untargeted LC-MS-Based Metabolic Fingerprinting of Escherichia Coli-Associated Urinary Tract Infections and Urosepsis: Insights into the Urine, Serum, and Bacterial Interactomes Urinary tract infections (UTIs) and urosepsis necessitate a deeper understanding of host–pathogen interactions at the metabolic level. We use LC-MS an…

Untargeted LC-MS-Based Metabolic Fingerprinting of Escherichia Coli-Associated Urinary Tract Infections and Urosepsis: Insights into the Urine, Serum, and Bacterial Interactomes #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

4 weeks ago

Development and validation of an efficient and sensitive LC-MS/MS assay for simultaneous determination of the high frequency used antibody drug conjugate payload MMAE and anti-vascular tyrosine kinase... Antibody drug conjugates (ADCs)-based combination therapies are increasingly studied in clinics. Monomethyl auristatin E (MMAE) is the most frequently…

Development and validation of an efficient and sensitive LC-MS/MS assay for simultaneous determination of the high frequency used antibody drug conjugate payload MMAE and anti-vascular tyrosine kinase inhibitor lenvatinib in human plasma #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

4 weeks ago

Ultra-sensitive HPLC–ESI–MS/MS quantitation of N-nitroso-phenylephrine in phenylephrine-containing anticold products Phenylephrine is widely used in anticold medications and contains a secondary amine moiety capable of forming N-nitroso-phenylephrine (NNPE), a potent…

Ultra-sensitive HPLC-ESI-MS/MS quantitation of N-nitroso-phenylephrine in phenylephrine-containing anticold products #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Metabolic profile of 25E-NBOH in human liver microsomes, rat urine, and fungus Cunninghamella elegans N-benzylphenethylamines are a class of new psychoactive substances (NPS) that are increasingly being used as recreational drugs with a wide range of a…

Metabolic profile of 25E-NBOH in human liver microsomes, rat urine, and fungus Cunninghamella elegans #JPBA #MassSpec www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Development of a MS binding assay to analyze the interactions of drugs with the ifenprodil binding site of NMDA receptors with GluN2B subunit Negative allosteric modulators interacting with the ifenprodil binding site of NMDA receptors with GluN2B subunit are of particular interest for the t…

Development of a MS binding assay to analyze the interactions of drugs with the ifenprodil binding site of NMDA receptors with GluN2B subunit #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Development and validation of an efficient and sensitive LC-MS/MS assay for simultaneous determination of the high frequency used antibody drug conjugate payload MMAE and anti- vascular tyrosine kinas... Antibody drug conjugates (ADCs)-based combination therapies are increasingly studied in clinics. Monomethyl auristatin E (MMAE) is the most frequently…

Development and validation of an efficient and sensitive LC-MS/MS assay for simultaneous determination of the high frequency used antibody drug conjugate payload MMAE and anti- vascular tyrosine kinase inhibitor lenvatinib in human plasma #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Counterfeit Thyroid Medications: A Comprehensive Analysis of the Composition of Products Seized by Law Enforcement Using Liquid Chromatography Coupled with Mass Spectrometry Drug counterfeiting is a global public health challenge affecting both developed and developing countries. This study reports the first comprehensive …

Counterfeit Thyroid Medications: A Comprehensive Analysis of the Composition of Products Seized by Law Enforcement Using Liquid Chromatography Coupled with Mass Spectrometry #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Integrating Non-targeted Metabolomics and Machine Learning for Comprehensive Phytochemical Profiling and Intelligent Discrimination of Acorus tatarinowii and Its Adulterants The dried rhizome of Acorus tatarinowii Schott (SCP) is a valued medicinal material in traditional Chinese medicine (TCM). However, its quality and ef…

Integrating Non-targeted Metabolomics and Machine Learning for Comprehensive Phytochemical Profiling and Intelligent Discrimination of Acorus tatarinowii and Its Adulterants #JPBA #MassSpec www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

In vitro Metabolic Profiling of Weight-Loss-Inducing Amylin Receptor Agonists in the Context of Preventive Doping Research Peptide hormone-based weight-loss therapeutics have gained increasing attention, driven amongst other reasons, by the clinical and commercial success …

In vitro Metabolic Profiling of Weight-Loss-Inducing Amylin Receptor Agonists in the Context of Preventive Doping Research #JPBA #MassSpec www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Development and validation of a robust UPLC-MS/MS method for the analysis of polyamines in cells, biofluids and tissues Loss of function (LoF) of the ATP13A2 protein, a polyamines transporter, has been linked to lysosomal and mitochondrial dysfunctions that play an impo…

Development and validation of a robust UPLC-MS/MS method for the analysis of polyamines in cells, biofluids and tissues #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Integrating plasma metabolomics using UPLC-Q Exactive Orbitrap HRMS and targeted amino acid and neurotransmitter analysis in plasma and cerebrospinal fluid by LC-MS/MS to explore biomarkers in pregnan... Insomnia is a common sleep issue among pregnant women. This study aimed to explore the metabolic characteristics of pregnant women with insomnia and i…

Integrating plasma metabolomics using UPLC-Q Exactive Orbitrap HRMS and targeted amino acid and neurotransmitter analysis in plasma and cerebrospinal fluid by LC-MS/MS to explore biomarkers in pregnancy women with insomnia #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Development and validation of a robust UPLC-MS/MS method for the analysis of polyamines in cells, biofluids and tissues Loss of function (LoF) of the ATP13A2 protein, a polyamines transporter, has been linked to lysosomal and mitochondrial dysfunctions that play an impo…

Development and validation of a robust UPLC-MS/MS method for the analysis of polyamines in cells, biofluids and tissues #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Metabolomics technology combined with O2PLS analysis reveals the active components of Paeoniae Radix Alba in preventing renal damage in rheumatoid arthritis Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by symmetrical, erosive polyarthritis and multi-organ involvement, with renal…

Metabolomics technology combined with O2PLS analysis reveals the active components of Paeoniae Radix Alba in preventing renal damage in rheumatoid arthritis #JPBA #MassSpec www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Integrating plasma metabolomics using UPLC-Q Exactive Orbitrap HRMS and targeted amino acid and neurotransmitter analysis in plasma and cerebrospinal fluid by LC-MS/MS to explore biomarkers in pregnan... Insomnia is a common sleep issue among pregnant women. This study aimed to explore the metabolic characteristics of pregnant women with insomnia and i…

Integrating plasma metabolomics using UPLC-Q Exactive Orbitrap HRMS and targeted amino acid and neurotransmitter analysis in plasma and cerebrospinal fluid by LC-MS/MS to explore biomarkers in pregnancy women with insomnia #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Exploring potential mechanism of Chuanzhi Qingyu Recipe for vulnerable atherosclerosis plaques based on UPLC-Q-TOF-MSE, systems biology strategy, and experimental validation Chuanzhi Qingyu (CZQY) recipe is clinically used for the secondary prevention and treatment of cardiovascular diseases associated with atherosclerosis…

Exploring potential mechanism of Chuanzhi Qingyu Recipe for vulnerable atherosclerosis plaques based on UPLC-Q-TOF-MSE, systems biology strategy, and experimental validation #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Fecal Metabolic Biomarkers Associated with Insomnia Severity: A Study on 5-Hydroxyindoleacetic Acid, Octopamine, Oleylethanolamide, and Elaidic Acid Insomnia is increasingly recognized as a disorder with complex metabolic underpinnings. We investigated the changes in fecal metabolite levels of 5-hy…

Fecal Metabolic Biomarkers Associated with Insomnia Severity: A Study on 5-Hydroxyindoleacetic Acid, Octopamine, Oleylethanolamide, and Elaidic Acid #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Development and validation of an UPLC–MS/MS method for simultaneous determination of meropenem and its open-ring metabolite in human serum and cerebrospinal fluid with application to clinical samples Central nervous system (CNS) infections require adequate drug exposure at the site of action, yet antibiotic meropenem (MER) shows limited cerebrospin…

Development and validation of an UPLC–MS/MS method for simultaneous determination of meropenem and its open-ring metabolite in human serum and cerebrospinal fluid with application to clinical samples #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Development and validation of a sensitive and rapid UHPLC-MS/MS method for the simultaneous quantification of CG-0255 and its active metabolite in human plasma and its application to Phase I studies CG-0255, a thiol prodrug of clopidogrel’s active metabolite H4 (CG-0236), is a novel thienopyridine P2Y12 receptor antagonist under initial clinical d…

Development and validation of a sensitive and rapid UHPLC-MS/MS method for the simultaneous quantification of CG-0255 and its active metabolite in human plasma and its application to Phase I studies #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Determination of Antigen Components in Inactivated SARS-CoV-2 Vaccine using Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry Accurate and reliable quantification of antigen components in vaccines is critical in vaccine quality control and evaluation of immunogenic consistenc…

Determination of Antigen Components in Inactivated SARS-CoV-2 Vaccine using Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

1 month ago

Detection and localization of single-nucleotide mutations in synthetic oligonucleotides by ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry Accurate detection of point mutations in mitochondrial DNA (mtDNA) is crucial for diagnosing various mitochondrial disorders. In this study, we develo…

Detection and localization of single-nucleotide mutations in synthetic oligonucleotides by ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 months ago

Comprehensive pharmacokinetic and tissue distribution study of α,ω-dipropionic acid polyethylene glycol (PA-PEG₁₂-PA) in rats using a validated UPLC-MS/MS method While polyethylene glycol (PEG) is an extensively utilized pharmaceutical polymer with established biocompatibility and regulatory acceptance, the in …

Comprehensive pharmacokinetic and tissue distribution study of α,ω-dipropionic acid polyethylene glycol (PA-PEG₁₂-PA) in rats using a validated UPLC-MS/MS method #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 months ago

Metabolic profiling of Gegen-Qinlian Decoction and its modified formula in gut microbiota from healthy individuals and ulcerative colitis patients with different syndromes using UHPLC-QTOF-MS/MS and a... The efficacy of Gegen-Qinlian Decoction (GQD) and its modified formula (MGQD) against ulcerative colitis (UC) with damp-heat syndrome (UC-DHS) is clos…

Metabolic profiling of Gegen-Qinlian Decoction and its modified formula in gut microbiota from healthy individuals and ulcerative colitis patients with different syndromes using UHPLC-QTOF-MS/MS and an in vitro incubation model #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 months ago

Enantioselective high-performance liquid chromatography-tandem mass spectrometry method for bioanalysis and chemical and stereochemical stability study of N-ethylpentedrone The first high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for enantioselective analysis of psychoact…

Enantioselective high-performance liquid chromatography-tandem mass spectrometry method for bioanalysis and chemical and stereochemical stability study of N-ethylpentedrone #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 months ago

Biopharmaceutical Assessment of Naloxone Permeation Through Human Respiratory Epithelial Tissues: A Chromatographic-Mass Spectrometric Approach with Cloud based Aerosol dosing and delivery Understanding naloxone permeation is important for optimizing nasal delivery and supporting comparative assessment of nasal drug products. In this stu…

Biopharmaceutical Assessment of Naloxone Permeation Through Human Respiratory Epithelial Tissues: A Chromatographic-Mass Spectrometric Approach with Cloud based Aerosol dosing and delivery #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 months ago

LC-MS peptide mapping of monoclonal antibodies using the mirror proteases trypsin and Tryp-N Accurate characterization of the amino acid sequence and post-translational modifications (PTMs) of monoclonal antibodies (mAbs) is essential for eval…

LC-MS peptide mapping of monoclonal antibodies using the mirror proteases trypsin and Tryp-N #JPBA www.sciencedirect.com/science/arti...

BioMassSpec

@realbiomassspec.bsky.social

2 months ago

Identification and characterization of trametinib degradation product employing Orbitrap LC-HRMS, and development of a robust, eco-friendly stability indicating method of analysis Trametinib is a selective MEK1/2 inhibitor approved for the treatment of various cancers. Stress testing of a drug is essential for understanding the …

Identification and characterization of trametinib degradation product employing Orbitrap LC-HRMS, and development of a robust, eco-friendly stability indicating method of analysis #JPBA www.sciencedirect.com/science/arti...