#NatProtoc

High-throughput reaction screening using acoustic ejection mass spectrometry - Nature Protocols A bottleneck in high-throughput chemical synthesis is analyzing reaction products. This protocol describes a direct mass spectrometry approach where the loss of a characteristic neutral fragment from ...

High-throughput reaction screening using acoustic ejection mass spectrometry #NatProtoc www.nature.com/articles/s41...

Client Challenge

Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level #NatProtoc www.nature.com/articles/s41...

Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection - Nature Protocols This robust, proteome-wide approach for the exploration of the S-nitrosoproteome in human and mouse tissues uses the SNOTRAP probe and nano-liquid chromatography–tandem mass spectrometry analysis. The...

Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection #NatProtoc www.nature.com/articles/s41...

Microfluidic #BloodBrainBarrier
hiPSC Endothelial Cells or primary brain microvascular ECs +primary brain pericytes & astrocytes

A protocol for microscopy-based permeability assay (minutes)
10/40 kD dextran

#NatProtoc 2022
www.nature.com/articles/s41...

A versatile toolkit for drug metabolism studies with GNPS2: from drug development to clinical monitoring - Nature Protocols This protocol extension guides users in identifying candidate drug metabolites without prior knowledge by analyzing tandem mass spectrometry data in the web-based molecular networking tool GNPS2.

A versatile toolkit for drug metabolism studies with GNPS2: from drug development to clinical monitoring #NatProtoc #MassSpec www.nature.com/articles/s41...

Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids - Nature Protocols We present a protocol for the analysis of polar and ionic metabolites by using anion-exchange chromatography–tandem mass spectrometry (AEC-MS/MS) with an untargeted or semi-targeted workflow for the i...

Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids #NatProtoc #MassSpec www.nature.com/articles/s41...

Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas - Nature Protocols It is important to understand how nanoparticles interact with biological systems and behave in vivo. This protocol describes top-down proteomics for protein corona profiling using advanced capillary z...

Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas #NatProtoc www.nature.com/articles/s41...

High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe - Nature Protocols We present a protocol for the design, fabrication and use of a microfluidic probe for nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) that achieves a spatial res...

High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe #NatProtoc www.nature.com/articles/s41...

Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles - Nature Protocols Nanoparticle pharmacokinetics is affected by the formation of a protein corona in vivo. Mass spectrometry-based proteomics is essential for understanding the corona composition. This Perspective discu...

Improving accuracy and reproducibility of mass spectrometry characterization of protein coronas on nanoparticles #NatProtoc www.nature.com/articles/s41...

Direct analysis of biotransformations with mass spectrometry—DiBT-MS - Nature Protocols Engineering enzymes capable of performing chemical transformations requires high-throughput assays to screen activity. In this protocol, reactions in cell lysates are analyzed directly by desorption e...

Direct analysis of biotransformations with mass spectrometry—DiBT-MS #NatProtoc www.nature.com/articles/s41...

A guide to reverse metabolomics—a framework for big data discovery strategy - Nature Protocols In this reverse metabolomics protocol, a tandem mass spectrometry spectrum is used as a search term to probe public metabolomic data. Analysis of the metadata connected with these search results enabl...

A guide to reverse metabolomics-a framework for big data discovery strategy #NatProtoc www.nature.com/articles/s41...

An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF) - Nature Protocols Aligning trapped ion mobility with a mass-selective quadrupole and time-of-flight mass spectrometry enables parallel accumulation–serial fragmentation, which improves proteomic analysis. This protocol...

An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF) #NatProtoc www.nature.com/articles/s41...

PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics - Nature Protocols This protocol outlines a cost-effective and rapid protein sample pre-fractionation strategy based on highly efficient passive elution from gels, recovering intact proteins for top-down proteomics or p...

PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics #NatProtoc www.nature.com/articles/s41...

Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography–mass spectrometry - Nature Protocols With this quantitative liquid chromatography–mass spectrometry-based assay designed for the detection of intact acyl-tRNA products, users can accurately discriminate acyl from non-acyl-tRNA, identify ...

Direct and quantitative analysis of tRNA acylation using intact tRNA liquid chromatography-mass spectrometry #NatProtoc www.nature.com/articles/s41...

Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers - Nature Protocols Orbitrap-based individual ion mass spectrometry enables charge detection mass spectrometry application on a broadly accessible mass spectrometry platform, enabling the analysis of complex mixtures tha...

Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers #NatProtoc www.nature.com/articles/s41...